Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094

January 2014 Vol. 3(1): pp. 024-034

Copyright © 2014 Global Advanced Research Journals

 

Full Length Research Paper

Genetic Diversity of Coffee (Coffea arabica L.) Landraces from Southern Ethiopia as Revealed by Inter Simple Sequence Repeat Marker

1 Shimekit Tadele, 2Firew Mekbib and 3Kassahun Tesfaye

 

1 Adama Science and Technology University, School of Natural Science, Department of Biology, P.O. Box: 1888: Adama, Ethiopia.

2Harmaya University, College of Agriculture, Plant Science Department, P.O. Box: 138, Dire Dawa, Ethiopia.

3 Addis Ababa University, College of Natural Sciences, Microbial, Cellular, and Molecular Biology Program Unit, P.O. Box: 1176, Addis Ababa, Ethiopia.

Corresponding Author’s E-mail: shimekit.tadeleg@gmail.com

Tel: +251910080830; Fax: 02510221100038

Accepted 04 January, 2013

 

Abstract

Despite its importance, the genetic variability of landraces Coffea arabica L. from Southern Ethiopia has not yet been examined well with molecular markers. Hence, in this study the genetic diversity of 87 coffee germiplasms from Southern Ethiopia was studied using five Inter Simple Sequence Repeats (ISSRs) primers. The proportion of polymorphic bands within populations varied from 20 % - 100 %, with a mean of 62.8 %. The Shannon’s diversity index varied from 0.11 to 0.55 with an overall mean of 0.33 and 0.47 for the entire data. The Nei's gene diversity ranges from 0.07 to 0.38 with the overall mean values across population and the mean for the entire data of 0.22 and 0.3, respectively. Analysis of Molecular Variance revealed 78.05% within while 21.95% variation is among population variation at (P=0.00). Neighbor Joining and Unweighted Pair Group Method using Arithmetic Averages (UPGMA) analysis showed most of the individuals fails to cluster on the basis of their respective populations while few individual grouped with others. UPGMA analysis of the populations revealed two major groups (Debub Omo and Gamo Gofa). Principal coordinate analysis showed most of the individual accessions that represent different populations spread all over the plot except few from Dawuro and Debub Omo that showed some tendency of grouping based on geographic origins. The data clearly indicated the existence of higher diversity that deserves conservation attention. Some populations are also observed to be more uniform which could be caused by specialty coffee extension program. 

Keywords: Cffea arabica; germplasm; Jaccard coefficient; polymorphism; repeat motifs; ISSR; Shannon’s index.

 

 

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