Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094

January 2014 Vol. 3(1): pp. 008-015

Copyright © 2014 Global Advanced Research Journals

Full Length Research Paper

Isolation of phosphate solubilizing bacteria from tropical soil

S. Asuming-Brempong and N.K. Aferi

Department of Soil Science, College of Agricultural and Consumer Sciences, University of Ghana.

Corresponding Author’s Email: sbrempong@yahoo.com

Accepted 16 December, 2013

Abstract

Phosphate solubilizing microorganisms were isolated from the Typic Paleudalf and the Pellustert.  Isolates obtained were tested for their ability to solubilize calcium phosphate, iron phosphate, and rock phosphate in Pikovskaya (1948) cultural medium. Most of the isolates were able to solubilize Fe-P, and then the Ca-P medium but little rock phosphate. There were no differences in solubility of inorganic phosphates between the isolates from the different soils types. The colonies of the isolates produced yellow halo zone on agar plates that bromothymol blue had been added indicating the production of weak organic acid. 

Keywords: Phosphate solubilizing bacteria, Pikovskaya medium, Paleudalf, rock phosphate.