Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
January 2020 Vol. 9(1): pp. 001-010
Copyright © 2020 Global Advanced Research Journals
Full Length Research Paper
Effect of high temperature on rhizobia survival on different leguminous seeds inoculated with liquid formulations
Somaya S. Mohamed, Mohammed A. Hassan, Migdam E. Abdelgani
Environment, Natural Resources and Desertification Research Institute. National Centre for Research, Sudan
*Corresponding Author's Email: firstname.lastname@example.org
Accepted 19 January, 2020
Liquid inoculants formulated with different polymeric additives viz., polyvinylpyrrolidone PVP, polyethylene glycol (PEG), polyvinyl alcohol PVA and Gum Arabic were evaluated for their ability to support Rhizobium survival on coated seeds. Inoculated faba bean, alfalfa, chickpea, mung bean, guar and soybean seeds were incubated at high temperatures (40°C and 45°C) for 24 and 48 hour. The number of rhizobia per seed of each treatment was determined using the plate count method. Liquid inoculants containing 1% PEG was found to maintain the highest number of cells surviving on faba bean seeds giving 12.4% increase compared to charcoal-based inoculants. Liquid inoculants amended with 0.8% Gum Arabic and 0.1% PEG recorded the maximum population of about 2.4×106 and 2×106 cells/ alfalfa seed, respectively. Highest population 8×105 cells/ chickpea seed was recorded in liquid inoculant formulated with 0.5% Gum Arabic. For mung bean, liquid inoculants amended with 0.1% Gum Arabic was found to record a maximum population of about 1×106 and 9×105 cells/seed after 24 and 48 h of storage at 45°C, respectively. Liquid inoculants amended with 0.1% and 0.5% PVA were found to be better in supporting Rhizobium population on guar seeds than charcoal based inoculants after 48 h. Liquid inoculants containing 0.5 % PVA and 0.1 % Gum Arabic could support the survival of rhizobial cell to 9.6% and 8.1% over charcoal based inoculant after 24 and 48 h from inoculation of soybean seeds at 45°C. The study concluded that liquid formulations could either promote rhizobial population or sustain rhizobial number equivalent to charcoal based inoculants. Moreover, Survivability of liquid formulations varied with the strain, seed and polymeric additives.
Keywords: Polymers, Rhizobia, Temperature, Inoculation rate, seeds.
Bashan Y, de Bashan LE, Parbhu SR, Hernandez JP (2014). Advances in plant growth-promoting bacterial inoculants technology: formulations and practical perspectives (11998-2013). Plant and Soil. 378:1-33.
Catroux G, Hartmann A,Revellin C (2001).Trends in rhizobial inoculant production and use.Plant and Soil. 230:21-30.
Dayamani KJ, Brahmaprakash GP (2014). Influence of form and Concentration of the Osmolytes in Liquid Inoculants Formulations of Plant Growth Promoting Bacteria. International Journal of Scientific and Research Publications.4:(7):1-6
Deaker R, Roughley RJ, Kennedy I R (2006). Desiccation tolerance of rhizobia when protected by synthetic polymers.Soil Biology and Biochemistry.39:573-580.
Girisha HC, Brahmaprakash G P, Mallesha BC (2006). Effect of osmoprotectant (PVP-40) on survival of Rhizobiumin different inoculants formulation and nitrogen fixation in cowpea. Geobios. 33:151-156.
Gomathy M, Thangaraju M, Gunasekharan S, Gopal NO, Gopal H (2008). Method and quantity of liquid formulation of phosphobacteria required for seed inoculation. Pakistan Journal of Biological Sciences. 11: 86-98.
Hafeez FY, Asad S, Malik KA (1991). The effect of high temperature on Vignaradiata nodulation and growth with different bradyrhizobialstrains. Environmental and Experimental Botany. 31:285-294.
Hungria M, Vargas MAT (2000). Environmental factors affecting N2 fixation in grain legumes in the tropics, with an emphasis on Brazil. Field Crop Research. 65:151-164.
Kaljeet S, Keyeo F, Amir HG (2011). Influence of carrier materials and storage temperature on survivability of rhizobial inoculant. Asian Journal of Plant Sciences.10:331-337.
Leo Daniel AE, Venkateswarlu B, Suseelendra D, Praveen k\KG, Mir Hassan SK, Meenakshi T, Uzma Sultan SP, Lakshm iNM (2013). Effect of polymeric additives, adjuvants, surfactants on survival, stability and plant growth promoting ability of liquid bioinoculants. Journal of Plant Physiology and Pathology.1:2.
Lupwayi NZ, Olsen PE, Sande ES, Kayser HH, Collins MM, Singleton PW, Rice WA (2000). Inoculant quality and its evaluation. Field Crops Research. 65:59-270.
Roughley RJ, Gemell LG, Thompson JA, Brockwell J (1993). The number of Bradyrhizobium sp. (Lupinus) applied to the seed and its effect on rhizosphere colonization, nodulation and yield of lupin. Soil Biology and Biochemistry. 25:1453-1458.
Sandra CP, Rebeca BR (2015).Polymers selection for a liquid inoculants of Azospirillumbrasilense based on the Arrhenius thermodynamic model. African Journal of Biotechnology. 14(3): 2547-2553.
Singleton P, Keyser H, Sande E (2002).Development and evaluation of liquid inoculants. In: D. Herridge (ed.). Inoculants and Nitrogen Fixation of Legumes in Vietnam Australia: Sun Photoset Pty.pp52-66.
Somasegaran P, Hoben HJ (1994).Handbook for Rhizobia, Methods in Legume-Rhizobium Technology. New York: Springer-Verlag.
Tittabutr P (2005). Development of Rhizobial Liquid Inoculant Production. Ph.D. Thesis Biotechnology School of Biotechnology, Suranaree University of Technology, Thailand.
Tittabutr P, Payakaponga W, Teaumroonga N, Singletonb PW, Boonkerd N (2007). Growth, survival and field performance of bradyrhizobial liquid inoculant formulations with polymeric additives. Science Asia.33:69-77.
ValettiL, Angelini JG, Taurian T, Ibáñez FJ, Muñoz VL, Anzuay MS, Ludueña LM, Fabra A (2016). Development and field evaluation of liquid inoculants with native Bradyrhizobial strains for peanut production. African Crop Science Journal. 24(1):1-13.
- Somaya S. Mohamed on Google Scholar
- Somaya S. Mohamed on Pubmed
- Mohammed A. Hassan on Google Scholar
- Mohammed A. Hassan on Pubmed
- Migdam E. Abdelgani on Google Scholar
- Migdam E. Abdelgani on Pubmed