Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094

October 2014 Vol. 3(10): pp. 310-316

Copyright © 2014 Global Advanced Research Journals

 

Full Length Research Paper

Evaluation of the infection of cassava and Nicotiana benthamiana with cassava mosaic geminiviruses and their combinations for potential synergism

Thomas E. Njock

 

Department of Agronomic and Applied Molecular Sciences, Faculty of Agriculture and Veterinary Medicine, University of Buea, P.O. Box 63 Buea, Cameroon.

Email: tnjockeku@yahoo.com

Accepted 01 October, 2014

 

Abstract

Five test plants each of cassava and Nicotiana benthamiana were inoculated in vitro with 50 ng cassava mosaic geminivirus (CMG) deoxyribonucleic acid (DNA) of components A and B and their random combinations for strains African cassava mosaic-Cameroon virus (ACMV-CM), East African cassava mosaic Cameroon virus (EACMV-CM), East African cassava mosaic Uganda virus (EACMV-UG), Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV) were investigated for synergistic interactions by bombardment with gold particle-coated viral DNA using the biolistic particle delivery system giving a total of 80 plants. The plants were maintained in an insect-proof greenhouse and scored for infection one week post inoculation (wpi) for 8 weeks on a scale of 1-5. The experiment was replicated twice. Incidence of infection was higher on N. benthamiana than cassava plants. The highest infection was with ACMCV/ICMV (100 %) on N. benthamiana and this was not significant over ACMV-CM (98.8 %) and the positive control ACMV-CM/EACMV-CM (96.3 %). Thus the combination ACMV-CM/ICMV probably had a synergistic effect on N. benthamiana. On cassava, however, the highest infection was ACMV-CM/EACMV-CM (50 %) which was not significant over ACMV-CM/SLCMV (46.3 %). Similarly, viral effect was more severe on N. benthamiana than on cassava plants. The most severe effects were by SLCMV (2.81), SLCMV/EACMV-CM (2.42) and ACMV-CM (2.32). ACMV-CM/EACMV-CM had earlier been demonstrated on cassava to have a synergistic effect. This study demonstrated that the ACMV-CM/ICMV combination had a synergistic effect on N. benthamiana and thus a potential threat of significance in plant virus disease epidemiology and therefore subject to further investigation.

Keywords: cassava mosaic disease (CMD), cassava mosaic geminiviruses (CMGs), CMG combinations, synergism, cassava, N. benthamiana.

 

 

Related Articles


Original Research Articles
Abdulazeez A Abubakar, Hassan A Oladele, Adewole A Adejumoke, Fadairo J Kayode
Synergistic effect of combined extract of Bryophyllum pinnatum and Aloe barbadensis enhances anti-microbial activity in-vitro
Glo. Adv. Res. J. Agric. Sci. January 2014 Vol: 3(1): - [Abstract] [Full Text - PDF] (175 KB)
Nzigamasabo Aloys
Volatile compounds in Ikivunde and Inyange two Burundian cassava products
Glo. Adv. Res. J. Agric. Sci. April 2012 Vol: 1(1): - [Abstract] [Full Text - PDF] (116 KB)
Okechukwu Obodoechina E
Effectiveness of Direct Resource Delivery Policy to Crop Farmers A Case of Fadama III Project in Enugu State Nigeria
Glo. Adv. Res. J. Agric. Sci. February 2015 Vol: 4(2): - [Abstract] [Full Text - PDF] (145 KB)
Amamgbo, L.E.F., Akinpelu A.O., Omodamiro R., Nwakor F. N. and Ekedo T.O
Promotion and Popularization of Some Elite Cassava Varieties in Igbariam Anambra State: Implication for Food Security and Empowerment
Glo. Adv. Res. J. Agric. Sci. February 2016 Vol: 5(2): - [Abstract] [Full Text - PDF] (273 KB)
Njock TE and Sama V
Quantification and correlation of African cassava mosaic disease parameters on cassava genotypes (Manihot esculenta, Crantz) in Buea, Cameroon.
Glo. Adv. Res. J. Agric. Sci. April 2015 Vol: 4(4): - [Abstract] [Full Text - PDF] (189 KB)
Oladele Peter Kolawole
Evaluation of Cassava Tuber Resistance to Deformation
Glo. Adv. Res. J. Agric. Sci. June 2012 Vol: 1(3): - [Abstract] [Full Text - PDF] (204 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Thomas E Njock. on Google Scholar
Thomas E Njock. on Pubmed

Statistics

Viewed 833
Printed 254
Downloaded 505
Powered By iPortal Works