Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
October 2017 Vol. 6(10): pp. 310-313
Copyright © 2017 Global Advanced Research Journals
Full Length Research Paper
A new Rhabdo virus Infection in Maize Plantations in Turkey
Ankara University, Faculty of Agriculture, Department of Plant Protection, 06110 Ankara,Turkey
Accepted 05 October, 2017
Maize mosaicrhabdo virus (MMV) is devastating virus infection, causes serious crop losses, on the family Poaceae is especially on maize and transmitedby leaf hopper, Peregrinusmaidis. Samples were collected from leaves and stems of maize plants, from Bartın, Düzce, Sakarya and Zonguldak provinces of Turkey, in 2012-2013. They were tested against MMV antiserum by DAS-ELISA and also RT-PCR assay with a primers specific to glycoprotein gene of the virus, designed in our laboratory. 15 of the collected maize samples were found to be infected with MMV. Positive samples were mechanically inoculated to maize plant lets for propagation and maintain. MMV inoculated maize plants, show light mosaic symptoms were tested by ELISA and RT-PCR again, obtained bands were 470bp long. In electron microscopic observations, rhabdovirus-like particules were detected.So far to our knowledge, it is the first report of Maize mosaic rhabdovirus presence in Turkey.
Keywords: Maize, MMV, Rhabdovirus, RT-PCR, electromicrocopy.