Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
November 2015 Vol. 4(11): pp. 748-760
Copyright © 2015 Global Advanced Research Journals

 

Full Length Research Paper

Cytotoxic and Genotoxic Effects of some Narcotic plant extracts using Higher Plant Bioassay

Rehab M. Rizk; Magda I. Soliman and Eman M. EL-Zayat

 

Botany Department, Faculty of Science, Mansoura University, Egypt

Corresponding Author's Email: new_fm8@yahoo.com 

Accepted 06 November, 2015

 

Abstract

The present study has been carried out to investigate the mutagenic effects of three narcotic plant extracts using higher plants genotoxicity test. Three narcotic substances were tested namely: Bango, hashish and opium. Three concentrations for each narcotic substance applied for two different exposure times 24 hr and 48 hr were used to treat the root tips of Vicia faba according to the standard protocol for the plant assays established by International Program on Chemical Safety (IPCS) and the World Health Organization (WHO). In addition, biochemical and molecular studies for Vicia seeds previously were treated with narcotic plant extracts. Biochemical studies include protein profile using polyacrylamide gel electrophoresis (SDS-PAGE) technique. Molecular studies using inter simple sequence repeats DNA (ISSR-DNA) technique. The obtained results indicate that the three used narcotic substances had the ability to cause different mitotic changes  such as mitotic index, phase distribution to the production of large number of mitotic abnormalities. For SDS-PAGE analysis, the results revealed that the total number of bands of the three narcotic substances were 26 bands with molecular weights ranging from 15 to 96.3 KDa. Eight primers were used in ISSR analysis, the total number of bands 60 from which 36 monomorphic and 24 polymorphic.  

Keywords: Vicia faba, narcotic substances, opium, bango, hashish, ISSR, protein profile. 


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