Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
October 2019 Vol. 8(10): pp. 311-316
Copyright © 2019 Global Advanced Research Journals

 

Full Length Research Paper

Study of soil biologicals properties

Hamid kheyrodin

 

Assistant professor semnan university –Iran

*Corresponding Author's Email: saman.karim@univsul.edu.iq

Accepted 14 November, 2019

 

Abstract

Two strategies have been developed to improve DNA recovery in terms of yield, purity and unbiased representation of the microbial diversity. However, amplification of DNA from soil is often inhibited by co-purified contaminants. Furthermore, DNA is also suitable for PCR amplification using various DNA targets. This review presents an overview of the available methods to achieve this challenging objective. DNA was extracted from 100g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and isopropanol precipitation. 

Keywords:  Purification, Soil, DNA, RNA

 

 

 

 

REFERENCES

 

Burgmann H, Pesaro M, Widmer F, Zeyer J (2001). A strategy for optimizing quality and quantity of DNAextracted from soil. J Microbiol Meth. 2001; 45: 7–20.

Denet E,  Coupat-Goutaland B, Nazaret S, Pélandakis M, Favre- Bonté S (2017). Diversity of free-living amoebae in soils and their associated human opportunistic bacteria. Parasitol. Res., 116 (11) (2017), pp. 3151-3162

Dahal RH,  Chaudhary DK,  Kim J (2018). Rhodanobacter hydrolyticus sp. nov., a novel DNA- and tyrosine-hydrolysing gammaproteobacterium isolated from forest soilInt. J. Syst. Evol. Microbiol., 68 (8) (2018), pp. 2580-2586,

Shokri A,  Sarvi S, Daryani A, Sharif M  (2016). Isolation and genotyping of Acanthamoeba spp. as neglected parasites in north of Iran Korean J. Parasitol., 54 (4) (2016), pp. 447-453

Shi-Ying Z,  Cong F, Yong-Xia W, Yun-Sheng X, Wei X, Xiao-Long C (2018). Salt-tolerant and plant growth-promoting bacteria isolated from high-yield paddy soil. Can. J. Microbiol. (27) (2018),

Nan L, Jiang Z, Wei X (2014). Emerging microfluidic devices for cell lysis: A review. Lab Chip 2014, 14, 1060–1073.

Saburi E, Rajaii T, Behdari A, Kohansal MH, Vazini H (2017). Free-living amoebae in the water resources of Iran: a systematic review J. Parasit. Dis., 41 (4) (2017), pp. 919-928

Yamanouchi K, Arima H, Sakamoto Y, Kanto K, Kasai K, Ito K, Inaba T (2018). First report of the isolation of Balamuthia mandrillaris in the northern region of Japan Parasitol. Res., 117 (9) (2018), pp. 2895-2900

Yamanouchi K, Sakamoto Y, Fujioka M, Kasai K, Tsujiguchi T, Kimura T, Shimazu M, Kato A, Ito K (2018). The effects of Pantoea and Kosakonia isolated from buckwheat sprouts on obese mice Appl. Microbiol.: Open Access, 4 (1) (2018)

 

Zhang J, Xiao J, Li S, Ran W (2017). Manure amendment increases the content of nanomineral allophane in an acid arable soilSci. Rep., 7 (1) (2017), p. 14256

Accepted 14 November, 2019

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