Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
October 2019 Vol. 8(10): pp. 311-316
Copyright © 2019 Global Advanced Research Journals
Full Length Research Paper
Study of soil biologicals properties
Assistant professor semnan university –Iran
*Corresponding Author's Email: firstname.lastname@example.org
Accepted 14 November, 2019
Two strategies have been developed to improve DNA recovery in terms of yield, purity and unbiased representation of the microbial diversity. However, amplification of DNA from soil is often inhibited by co-purified contaminants. Furthermore, DNA is also suitable for PCR amplification using various DNA targets. This review presents an overview of the available methods to achieve this challenging objective. DNA was extracted from 100g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and isopropanol precipitation.
Keywords: Purification, Soil, DNA, RNA
Burgmann H, Pesaro M, Widmer F, Zeyer J (2001). A strategy for optimizing quality and quantity of DNAextracted from soil. J Microbiol Meth. 2001; 45: 7–20.
Denet E, Coupat-Goutaland B, Nazaret S, Pélandakis M, Favre- Bonté S (2017). Diversity of free-living amoebae in soils and their associated human opportunistic bacteria. Parasitol. Res., 116 (11) (2017), pp. 3151-3162
Dahal RH, Chaudhary DK, Kim J (2018). Rhodanobacter hydrolyticus sp. nov., a novel DNA- and tyrosine-hydrolysing gammaproteobacterium isolated from forest soilInt. J. Syst. Evol. Microbiol., 68 (8) (2018), pp. 2580-2586,
Shokri A, Sarvi S, Daryani A, Sharif M (2016). Isolation and genotyping of Acanthamoeba spp. as neglected parasites in north of Iran Korean J. Parasitol., 54 (4) (2016), pp. 447-453
Shi-Ying Z, Cong F, Yong-Xia W, Yun-Sheng X, Wei X, Xiao-Long C (2018). Salt-tolerant and plant growth-promoting bacteria isolated from high-yield paddy soil. Can. J. Microbiol. (27) (2018),
Nan L, Jiang Z, Wei X (2014). Emerging microfluidic devices for cell lysis: A review. Lab Chip 2014, 14, 1060–1073.
Saburi E, Rajaii T, Behdari A, Kohansal MH, Vazini H (2017). Free-living amoebae in the water resources of Iran: a systematic review J. Parasit. Dis., 41 (4) (2017), pp. 919-928
Yamanouchi K, Arima H, Sakamoto Y, Kanto K, Kasai K, Ito K, Inaba T (2018). First report of the isolation of Balamuthia mandrillaris in the northern region of Japan Parasitol. Res., 117 (9) (2018), pp. 2895-2900
Yamanouchi K, Sakamoto Y, Fujioka M, Kasai K, Tsujiguchi T, Kimura T, Shimazu M, Kato A, Ito K (2018). The effects of Pantoea and Kosakonia isolated from buckwheat sprouts on obese mice Appl. Microbiol.: Open Access, 4 (1) (2018)
Zhang J, Xiao J, Li S, Ran W (2017). Manure amendment increases the content of nanomineral allophane in an acid arable soilSci. Rep., 7 (1) (2017), p. 14256