Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
October 2019 Vol. 8(10): pp. 311-316
Copyright © 2019 Global Advanced Research Journals
Full Length Research Paper
Study of soil biologicals properties
Assistant professor semnan university –Iran
*Corresponding Author's Email: email@example.com
Accepted 14 November, 2019
Two strategies have been developed to improve DNA recovery in terms of yield, purity and unbiased representation of the microbial diversity. However, amplification of DNA from soil is often inhibited by co-purified contaminants. Furthermore, DNA is also suitable for PCR amplification using various DNA targets. This review presents an overview of the available methods to achieve this challenging objective. DNA was extracted from 100g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and isopropanol precipitation.
Keywords: Purification, Soil, DNA, RNA
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