Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094

December 2013 Special Anniversary Review Issue Vol. 2(12): pp. 322-324

Copyright © 2015 Global Advanced Research Journals

 

Full Length Research Paper

Effects of Herbicide Attribut (propoxycarbazone-sodium) and Surfactant BioPower (sodium alkylether-sulphate) on DNA amounts of Triticum aestivum L. cv. Pehlivan by Flow Cytometry.

Gulden Yılmaz1, Ozlem Aksoy2, Cıler Merıc1, Feruzan Dane1

 

1Trakya University, Faculty of Science, Department of Biology, Balkan Campus, 22030, Edirne/Turkey

2 Kocaeli University, Faculty of Science and Arts, Department of Biology, İzmit/Kocaelı

*Corresponding Author’s: Email: guldenyilmaz2009@yahoo.com

Accepted 15 December, 2013

 

Abstract

In the present study, the effects of herbicide Attribut (propoxycarbazone-sodium) and an anionic surfactant BioPower (sodium alkylether-sulphate) on DNA amounts of Triticum aestivum L. cv. Pehlivan were investigated and compared by flow cytometric method. For the experiments, Triticum aestivum were treated with Attribut at the concentrations of 0.21 mM, 0.42 mM, 0.82 mM and 1.68 mM, respectively. In order to evaluate the combined effect of  BioPower and Attribut, 0.25% BioPower was added in each of applied Attribut doses. The nuclear DNA content of these specimens was analyzed by flow cytometry. Propidium iodide-stained nuclei were analyzed on an EPICS XL (Beckmann Coulter) model flow cytometer. The nuclear DNA content (2C-value) of the treated specimens were given. DNA amount was shown to decrease in the treatment of (FD×4) 1.68 mM Attribut while it increased in the treatment of  (FDS×4) 1.68 mM Attribut + 0.25%  BioPower.   

Keywords: flow cytometry, genotoxic effect, propoxycarbazone-sodium, sodium alkylether-sulphate.

 

 

 


 

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