Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
February 2016 Vol. 5(2): pp. 067-087
Copyright © 2016 Global Advanced Research Journals

 

Full Length Research Paper

Intra- and inter-specific variations in some isoenzymes and in eye-lens nucleus and muscle proteins of three Lethrinus species from the Red Sea at Hurghada, Egypt.

Mekkawy, IAA1,2; Ahmed S. A. Harabawy 1; U. M. Mahmoud1

 

1Zoology Department, Faculty of Science, Assiut University, Assiut, Egypt.

2Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia.

Email: iaa_mekkawy@yahoo.co.uk

Accepted 27 January, 2016

 

Abstract

The level of genetic differentiation among Lethrinus species  was revealed by using the electrophoresis techniques.Three isoenzymes (LDH, G6PDH and  Esterases) and the general proteins (eye-lens nucleus and muscles proteins) were studied. The isoenzyme-electrophoretic results revealed that, LDH-isoenzyme patterns were found to be controlled by two loci: A, B for muscles and heart, while in the liver, in addition to gene loci A and B, a third locus C was detected and migrated toward the cathode.The muscle G6PDH isoenzyme patterns were explained to be influenced by 4 loci (A, B, C & D). However, the heart ones were encoded only by 2 loci (B&D). No ontogenetic variations in the esterase isoenzyme patterns of Lethrinus species were detected through the rang of length considered. The esterase molecules were presumed to be monomers and were coded by 3 loci (A, B and C); all these loci are common between muscles and heart.In the present work polymorphism or heterogeneity was detected in eye-lens nucleus protein patterns of Lethrinus species. Such protein heterogeneity  was expressed in four zones which can be explained by four genetic loci namely: EL-1, EL-2, EL-3 and EL-4. The present investigation revealed that polymorphism in muscle protein patterns was recorded only in L. mahsena and was lacking in L. borbonicus and L. nebulosus. The relatively genetic distances between L. borbonicus,  L. mahsena and L. nebulosus were estemated and the relative times of divergence between  Lethrinus species were estimated to be: 2,572,819 years for L. borbonicus and L. mahsena; 2,341,406 years for L. borbonicus and L. nebulosus; 3,105,669 years for L. mahsena and L. nebulosus.

Keywords: Electrophoresis, Isoenzymes, Proteins, Genetics, Lethrinus.


 

Related Articles


Original Research Articles
T.H. Mostafa, A.M. Abd El-Salaam, Mona E. Farag and M.M. Refaei
Effect of Feeding Treatments on Productive and Reproductive Performance of She-Camels
Glo. Adv. Res. J. Agric. Sci. July 2016 Vol: 5(7): - [Abstract] [Full Text - PDF] (352 KB)
Joeber Bernardo Soares de Souza, Cyro Teixeira da Silva Junior, Jorge Luiz Barillo, Patricia Siqueira Silva, Salim Kanaan, Elizabeth Giestal de Araujo and Analucia Rampazzo Xavier
Effects of the age and sex of an automated assay for adenosine deaminase among a Brazilian population sample with pleural tuberculosis
Glo. Adv. Res. J. Agric. Sci. October 2017 Vol: 6(10): - [Abstract] [Full Text - PDF] (246 KB)
Review
Najya A Attia
Neonatal Diabetes Mellitus: Clinical and Genetic Approach
Glo. Adv. Res. J. Agric. Sci. January 2018 Vol: 7(1): - [Abstract] [Full Text - PDF] (811 KB)
Original Research Articles
Hana M Gashlan and Asma B Al-Beladi
Effects of Clove Oil on Liver and Antioxidant Status of Streptozotocin-Induced Diabetic Rats
Glo. Adv. Res. J. Agric. Sci. June 2017 Vol: 6(6): - [Abstract] [Full Text - PDF] (853 KB)
Mahnaz Sadat Sadeghi, Sadegh Peery, Mehdi Hosseini
Mercury (Hg) concentration and distribution in selected tissues of some fish species from Arvand River, south Iran
Glo. Adv. Res. J. Agric. Sci. February 2018 Vol: 7(2): - [Abstract] [Full Text - PDF] (427 KB)
Mahmoud A. Al-Saman, Safinaz A. Farfour, Ahmed A. Tayel, Nashwa M. Rizk
Bioactivity of lectin from Egyptian Jatropha curcas seeds and its potentiality as antifungal agent
Glo. Adv. Res. J. Agric. Sci. August 2015 Vol: 4(7): - [Abstract] [Full Text - PDF] (693 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Mekkawy on Google Scholar
Mekkawy on Pubmed
IAA; Ahmed S. A. Harabawy; U. M. Mahmoud on Google Scholar
IAA; Ahmed S. A. Harabawy; U. M. Mahmoud on Pubmed

Statistics

Viewed 2193
Printed 524
Downloaded 1438
Powered By iPortal Works