Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
February 2017 Vol. 6(2): pp. 057-064
Copyright © 2016 Global Advanced Research Journals

 

Full Length Research Paper

Growth profiles of transgenic tobacco plants expressing rice 45S rRNA gene

So Makabe1, Kynet Kong2, Hiroyuki Niimi1, Ikuo Nakamura1

 

1 Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan

2 Cambodian Agricultural Research and Development Institute, Phnom Penh, Cambodia

Email: makabe@chiba-u.jp

Accepted 30 January, 2017

 

Abstract

Recently, we found that transgenic Arabidopsis plants with the forced expression of rice 45S rRNA gene enhanced 2-fold growth of above ground tissues. Thus, we made transgenic tobacco plants harboring rice 45S rRNA driven by maize ubiquitin promoter (UbiP::Os45SrRNA) or cauliflower mosaic virus 35S promoter (35SP::Os45SrRNA). Only whole plant size and dry weight of 35SP::Os45SrRNA and UbiP::Os45SrRNA transgenic tobacco plants were increased compared to those of the control plants. On the other hand, relative DNA content of leaf cell, size of stomata cell, and density of stomata per unit leaf area were similar between the transgenic and control plants. Transgenic tobacco plant harboring UbiP::Os45SrRNA and 35SP::Os45SrRNA transgene were increased dry weight to 2.1 and 1.4 fold compared to the control plant at 45 days after sowing (45 DAS), respectively. Transgenic plant harboring 35SP::Os45SrRNA transgene bloomed flowers at 107 DAS compared to 152 DAS of the control plant. Therefore, transgenic plants could produce the number of their fruits to 3 times than the control plant. Although concentrations of nicotine, a secondary metabolite of tobacco plant, in leaves were similar between transgenic and control plants, total amount of nicotine in the transgenic plant was produced more than twice than that in the control plant. Although the mechanism underlying the increased growth remains to be understood, forced expression of foreign 45S rRNA gene will contribute to the production of secondary metabolites of plants as well as the growth increase of cereals, vegetables, and bio-energy plants. 

Keywords: 45S ribosomal RNA, forced expression, growth increase, nicotine, transgenic plants.  

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