Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
February 2018 Vol. 7(2): pp. 053-063
Copyright © 2018 Global Advanced Research Journals


Full Length Research Paper

Estimation of antioxidant activity in natural rubber (H.brasiliensis) using TPC, FRAP, CUPRAC, ABTS, DPPH, ORAC assays, individual phenolic and individual flavonoid quantity 

Suwimon Siriwonga,b*, Adisai Rungvichaniwatb, Pairote Klinpituksab, Khalid Hamid Musac and Amina Abdullahc 


aCollege of Innovation and Management, Songkhla Rajabhat University, Songkla, 90000, Thailand

bDepartment of Rubber Technology and Polymer Science, Faculty of Science and Technology, Prince of Songkla University, Pattani campus, Pattani, 94000, Thailand

cSchool of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, 43600, Malaysia

*Corresponding Author's Email:

Accepted 18 February, 2018



Natural antioxidant in natural rubber is very useful to react with oxygen or ozone to protect natural rubber from undesirable chemical oxidations. There are various methods used to determine the quantity of antioxidant in food that may be applied to estimate natural antioxidant in natural rubber. The main objective of the present work is to study the effects of different extracted solvents (mixtures in the volume ratio of 4:1 of chloroform:acetone, chloroform:methanol, cyclohexane:acetone and cyclohexane:methanol) on the detected quantity of antioxidant in three grades of natural rubber; Air dry sheet (ADS), Ribbed smoked sheet No.3 (RSS3) and Standard thai rubber 20 (STR20). Various methods of the estimation of antioxidant in natural rubber were also investigated by using total phenolic content (TPC) assay, ferric reducing antioxidant power (FRAP) assay, cupric ion reducing antioxidant capacity (CUPRAC) assay, 2,2¢-Azinobis-(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) assay,  1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, oxygen radical absorbance capacity (ORAC), individual phenolic and individual flavonoid. It was found that the mixture of cyclohexane:methanol showed the highest antioxidant activity verified by TPC, FRAP, ABTS, DPPH and ORAC. Individual phenolic and flavonoid exhibited quite different among solvent types, phenolic types and flavonoid types. Moreover, almost all of the results showed that ADS exhibited lower antoxidant activity than that of STR20 and RSS3. 

Keywords: antioxidant, flavonoid, natural rubber, phenolic.





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