Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
February 2018 Vol. 7(2): pp. 034-045
Copyright © 2018 Global Advanced Research Journals


Full Length Research Paper

High Density of Transposable Elements in Sequenced Sequences in Cattle Genomes, Associated With AGC Microsatellites

1,2Valery Glazko, 1Gleb Kosovsky, 1,2Tatyana Glazko


1Research Institute of Fur Farming and Rabbit Breeding Industries n.a. V.A. Afanasyev, Moscow region, 140143, Russia

2Russian Timiryazev State Agrarian University — Moscow Agrarian Academy, Moscow, 127550, Russia

*Corresponding Author's Email:

Accepted 08 February, 2018



One of the urgent tasks of agricultural biotechnology is the selection of the most informative DNA markers for polylocus genotyping (genome scan) to control the consolidation and dynamics of gene pool of farm animal species, species origin, identification of the animals maximal promising in terms of productivity and adaptive potential. The study discusses some generations of molecular genetic markers that were applied to solve these problems. It presents the results of sequencing of "anonymous" DNA sequences that were used for polylocus genotyping on the basis of DNA fragments flanked by invert repeats of microsatellite loci (ISSR - Inter-Simple Sequence Repeat). The bovine genomic DNA fragments about 500 bp long flanked with inverted repeat (AGC)6G were sequenced. The regions with identity of more than 80% to SINE, LINE and ERV Bos taurus-specific retrotransposons were found in all the DNA fragments under study. We revealed a trend towards overrepresentation of the regions with identity to retrotransposons in the genomic sequences of bovine leucosis retrovirus-infected cows. The article discusses a link between increased polymorphism of the primer (AGC)6G amplification spectra and retrotransposon association of this microsatellite.

Keywords: ISSR—PCR—markers, sequencing, SINE, LINE, ERV, LTR, Bos taurus genome, polymorphism.





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