Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
February 2020 Vol. 9(2): pp. 027-039
Copyright © 2020 Global Advanced Research Journals
Full Length Research Paper
Perineal Plant Extract as a Culture Medium for Production of Plant Growth Regulators by Molecularly Identified Rhizospheric Microorganisms
Shimaa E. Ibrahim1, Hala F Mohamed1, Heba Sh. Shehata2, and Rawheya A. Salah El Din*
1Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt.
2Microbiology Department, Soils; Water and Environment Research Institute, Agriculture Research Center, Giza, Egypt.
*Corresponding Author's Email: firstname.lastname@example.org
Accepted 13 February, 2020
The feasibility of developing alternative environment friendly media for conventional chemical synthetic media was assessed using perineal plant extracts as locally available cheap materials which contain considerable amount of carbohydrates, proteins and macro elements necessary for microbial growth. In this study, about fifteen bacterial isolates were isolated on Aloe vera-based culture medium from Aloe vera rhizosphere and ten bacterial and eight actinomycetes isolates were isolated on Mentha-based culture medium form Mentha varidis rhizosphere. Out of all,MA6 and A13 isolated from Menthavaridis and Aloe vera rhizosphere respectively produce more gibberellic acid (27.86 and 17.32 µg/ml respectively) on natural medium. Additionally, both isolates were efficient in carbohydrate production MA6 (89µg/ml) and A13 (144µg/ml) on natural medium. Moreover, 9(50 %) isolates and 4(23%) isolates showed maximum Ammonia production qualitatively on peptone water after growing on Mentha-based culture medium and synthetic medium respectively. While 1(7%) and 7 (47%) isolates revealed maximum Ammonia production qualitatively on peptone water after growing on Aloe vera -based culture medium and synthetic medium. Phylogenetic analysis of 16S rRNA sequence of the two isolates MA6 and A13 showed maximum sequence similarity with Streptomyces rochei strain DW3 and Xanthomonas nasturtii strain WHRI 8853. Streptomyces rochei strain DW3 showed strong antifungal activity against Fusariumsolani (18 mm zone of inhibition) and Xanthomonas nasturtii strain WHRI showed good antifungal activity against Fusarium oxysporum (19 mm zone of inhibition). We hereby recommend switching to use of plant extracts which is environment friendly and also more economic for cultivation as an alternative for synthetic culture media.
Keywords: Plant-based medium, Perineal plants, rhizobacteria, Actinomycetes, PGP activities, antagonism.
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