Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
February 2020 Vol. 9(2): pp. 027-039
Copyright © 2020 Global Advanced Research Journals

 

Full Length Research Paper

Perineal Plant Extract as a Culture Medium for Production of Plant Growth Regulators by Molecularly Identified Rhizospheric Microorganisms

Shimaa E. Ibrahim1Hala F Mohamed1, Heba Sh. Shehata2, and Rawheya A. Salah El Din*

1Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt.

2Microbiology Department, Soils; Water and Environment Research Institute, Agriculture Research Center, Giza, Egypt.

*Corresponding Author's Email: elmonaliza96@gmail.com

Accepted 13 February, 2020

 

Abstract

The feasibility of developing alternative environment friendly media for conventional chemical synthetic media was assessed using perineal plant extracts as locally available cheap materials which contain considerable amount of carbohydrates, proteins and macro elements necessary for microbial growth. In this study, about fifteen bacterial isolates were isolated on Aloe vera-based culture medium from Aloe vera rhizosphere and ten bacterial and eight actinomycetes isolates were isolated on Mentha-based culture medium form Mentha varidis rhizosphere. Out of all,MA6 and A13 isolated from Menthavaridis and Aloe vera rhizosphere respectively produce more gibberellic acid (27.86 and 17.32 µg/ml respectively) on natural medium. Additionally, both isolates were efficient in carbohydrate production MA6 (89µg/ml) and A13 (144µg/ml) on natural medium. Moreover, 9(50 %) isolates and 4(23%) isolates showed maximum Ammonia production qualitatively on peptone water after growing on Mentha-based culture medium and synthetic medium respectively. While 1(7%) and 7 (47%) isolates revealed maximum Ammonia production qualitatively on peptone water after growing on Aloe vera -based culture medium and synthetic medium. Phylogenetic analysis of 16S rRNA sequence of the two isolates MA6 and A13 showed maximum sequence similarity with Streptomyces rochei strain DW3 and Xanthomonas nasturtii strain WHRI 8853. Streptomyces rochei strain DW3 showed strong antifungal activity against Fusariumsolani (18 mm zone of inhibition) and Xanthomonas nasturtii strain WHRI showed good antifungal activity against Fusarium oxysporum (19 mm zone of inhibition). We hereby recommend switching to use of plant extracts which is environment friendly and also more economic for cultivation as an alternative for synthetic culture media.

Keywords: Plant-based medium, Perineal plants, rhizobacteria, Actinomycetes, PGP activities, antagonism. 

 

 

 

 

REFERENCES

 

Ali RS, Rifat H, Zhang X, Nadeem AA, Safdar A, MukhtarA, Jabar ZKK, Shakeel A (2019): Exploring Potential Soil Bacteria for Sustainable Wheat (Triticumaestivum L.) production. Sustainability,11(3361):1-12.

Antony B, Naik A, Karicheri R (2014): Soychunk extract agar (sea) new bacteriological medium - a preliminary report, World Journal of Pharmaceutical Sciences, 3: 796-800.

Arulanantham R,  Pathmanathan S, Ravimannan N, Niranjan K (2012): Alternative culture media for bacterial growth using different formulation of protein sources. Journal of Natural Product and Plant Resources,2: 697-700.

Borgio JF, Bency, BJ, Ramesh S, Amuthan M (2009): Exopolysaccharide production by Bacillus subtilis NCIM 2063, Pseudomonas aeruginosa NCIM 2862 and Streptococcus mutans MTCC 1943 using batch culture in different media. The African Journal of Biotechnology,9(20):5454-5457.

Cappuccino JC, Sherman N (1992): In Microbiology:  A Laboratory Manual, 3 Edn, Benjamin/cummings Pub. Co. New York, 125–179.

Claverie JM, Notredame C (2003): Bioinformatics for Dummies. Wiley Publishing, New York.

Coplin DL, Cook D (1990): Molecular genetics of extra cellular polysaccharide biosynthesis in vascular phytopathogenic bacteria. Molecular Plant-Microbe Interactions, 3:271–279.

Dubois M, Gilles KA, Hamilton JK, Rebers P A, Smith F (1956): Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, 28(3): 350-356.

Dye DW (1962): The inadequacy of the usual determinative tests for identification of Xanthomonas spp.  New Zealand journal of science, 5:393 – 416. 

Edwards U, Rogall T, Blo¨ cker H, Emde M, Bo¨ ttger EC (1989): Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA.Nucleic Acids Research, 17: 7843–7853.

GoudjalY, Toumatia O,Yekkour A,Sabaou N, Mathieu F, Zitouni A (2014): Biocontrol of Rhizoctonia solani damping-off and promotion of tomato plant growth by endophytic actinomycetes isolated from native plants of Algerian Sahara. Microbiological Research, 169: 59–65.

Gusmiaty, Restu M, Bachtiar B,  Larekeng SH (2019): Gibberellin And IAA Production by Rhizobacteria From Various Private Forest. IOP Conf. Series: Earth and Environmental Science, 270 : 012018.

Ibrahim SE, Ahmed EA, Shehata HSh, El Shahat RM, Salah El Din R A (2016): Isolation of Rhizobacteria and Micro Algae from Saline Soil and Production of Plant   Growth Promoters. Global Adv. Res. J. Microbiol., 5(3): 23-31. 

Jog R, Nareshkumar G, Rajkumar S (2012): Plant growth promoting potential and soil enzyme production of the most abundant Streptomyces spp. from wheat rhizosphere. Journal of Applied Microbiology, 113: 1154-1164.

Jukes TH, Cantor CR (1969): Evolution of protein molecules.In Mammalian protein metabolism (ed. H.N. Munro). Academic Press, New York. pp. 21-123.

Kawato M, Shinobu R (1959): A simple technique for the microscopically observation. Memoirs of the Osaka University Liberal Arts and Education, 8: 114.

Kumer SP, Shyam CRC (2014): Efficacy of Pseudomonas as biocontrol agent against plant pathogenic fungi. International journal on current microbiology and applied science, 3(11) 493-500.

Madigan MT, Martinko JM, Parker J (1997): Antibiotics: isolation and characterization. In: Brock Biology of Microorganisms, 8th (ed), Prentice-Hall International Inc. New Jersey, 440-442.

Mainasara MM, Abu Bakar MF, Waziri AH, Musa AR (2018):Comparison of Phytochemical, Proximate and Mineral Composition of Fresh and Dried Peppermint (Mentha piperita) Leaves. Journal of Science and Technology, 10 (2): 85-91.

 Manjanna K, Shivakumar B, Pramodkumar T (2009): Natural exopolysaccharides as novel excipients in drug delivery: A review. Archives of Applied Science Research, 1:230–253.

Nirmala C, Bisht MS, Laishram M (2014): Bioactive compounds in bamboo shoots: health benefits and prospects for developing functional foods. International Journal of Food Science and Technology, 49(6):1425–1431.

Nour EH, Hamza MA, Fayez M, Monib M, RuppelS, Hegazi NA (2012): The crude plant juices of desert plants as appropriate culture media for the cultivation of rhizospheric microorganisms. Journal of Advanced Research, 3(1): 35-43.

Osman ZA, Elsanousi SM, Elsheikh EAE (2012): Plant materials as probable growth promoters for certain fungi. European Journal of Experimental Biology, 2: 1785-1791.

Patel T and Saraf M (2017): Exploration of Novel Plant Growth Promoting Bacteria Stenotrophomonasmaltophilia MTP42 Isolated from the Rhizospheric Soil of Coleus forskohlii. International Journal of Current Microbiology and Applied Sciences, 6(11):944-955.

Pellizzoni M, Ruzickova G, Kalhotka L, Lucini L (2012): Antimicrobial activity of different Aloe barbadensis Mill. And Aloe arborescens Mill.leaf fractions.Journal of Applied medicinal plant research, 6: 1975–1981.

Ravathie A, Sevvel P, Nirmala R, Kularajany N (2012):Alternative culture media for bacterial growth using different formulation of protein sources  Journal of natural product and plant resources, 2(6): 697-700.

Santosa S, Sutarno, Purwanto E, Suranto, Sajidan (2018):  Molecular characterization of Plant Growth Promoting Rhizobacteria using 16S rRNA sequences in the organic rice field of Sukorejo Village, Central Java, Indonesia. Biodiversitas, 19 (6): 2157-2162.

Shameer S, Prasad TNVKV (2018):Plant growth promoting rhizobacteria for sustainable agricultural practices with special reference to biotic and abiotic stresses.Plant Growth Regulation, 84:603–615.

Sharma S, Sharma A, Kaur M (2018): Extraction and evaluation of gibberellic acid from Pseudomonas sp.: Plant growth promoting rhizobacteria. Journal of Pharmacognosy and Phytochemistry, 7(1): 2790-2795.

Shindy WW, Smith OF (1975): Identification of plant hormones from cotton ovules. Plant Physiology, 55: 550-554.

Silosuch LA, Lethbridge BJ, Raffet SJ, He H, Clardy J, Handelsman J (1994): Biological activities of two fungi static antibiotic produced by Bacillus cereus UW85. Applied and Environmental Microbiology, 60: 2023-2030.

Tamura K, Dudley J, Nei M, Kumar S (2007): MEGA4, Molecular Evolutionary Genetics Analysis (MEGA) Software version 4.0. Molecular Biology and Evolution,24 (8): 1596-1599.

Thompson JD, Higgins DG, Gibson TJ (1994): CLUSTAL W—Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrixchoice. Nucleic Acids Research, 22: 4673–4680.

Tiwari M, Upadhayay M (2018): The medicinal plant components and applications (Aloe vera). Journal of Medicinal Plants Studies,6(3): 89-95.

Udagwa K, Kinoshita S (1961): A colorimetric determination of gibberellic acid. Journal of the Agricultural Chemical Society of Japan, 35: 219-223.

Uthayasooriyan M, Pathmanathan S, Ravimannan N, Sathyaruban S (2016): Formulation of alternative culture media for bacterial and fungal growth. Der Pharmacia Lettre, 8 (1):431-436.

Veerapagu M, Jeya KR, Priya R. Vetrikodi N (2018): Isolation and screening of plant growth promoting rhizobacteria from rhizosphere of chilli. Journal of Pharmacognosy and Phytochemistry,7(4): 3444-3448.

Vincent JM (1970):  A manual for the practical study of the root           nodule bacteria. Burgess and Son Ltd. Great Britain, pp. 45.

Zamoum M, Goudjal Y, Sabaou N, Barakate M, Mathieu F, Zitouni A (2015): Biocontrol capacities and plant growth-promoting traits of endophytic actinobacteria isolated from native plants of Algerian Sahara. Journal of Plant Diseases and Protection, 122: 215–223.

Zamouma MB, Goudjala YB, Sabaoua N, Mathieuc F, Zitouni A (2017): Development of formulations based on Streptomyces rochei strain PTL2 spores for biocontrol of Rhizoctonia solani damping-off of tomato seedlings. Biocontrol Science and Technology, 27(6):723-738.

 

 

 

 

 

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