Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
March 2018 Vol. 7(3): pp. 071-079
Copyright © 2018 Global Advanced Research Journals

 

Full Length Research Paper

Evolutive phylogenetic based on amino acids sequence surround the fusion protein cleavage site gene of Newcastle disease virus from field samples of surveillance program and vaccine strains in Brazil

Maria Angela Orsi1,2*, Luciana Helena Antoniassi da Silva1, Clarice Weis Arns 1 and Tânia Rosária Pereira Freitas3

 

1 Animal Virology Laboratory, Institute of Biology, University of Campinas, UNICAMP, Campinas;

2 Animal and Plant Health National Laboratory of  São Paulo, LANAGRO-SP, Campinas, Brazil;

3Animal and Plant Health National Laboratory of Minas Gerais, LANAGRO-MG, 33600-000 Pedro Leopoldo, MG, Brazil.

*Corresponding Author's Email: giulia96@yahoo.com

Accepted 02 March, 2018

 

Abstract

The AAvV-1 official surveillance provided an opportunity for the survey of infections in poultry for internal consume and to exportation, applying molecular methods. In this work, the RT-PCR and direct sequencing of partial fusion protein gene from AAvV-1isolates from poultry production of different Brazilian geographic areas as from live vaccines applied in each region were performed.  The deduced amino acid sequences were inferred and phylogenetically compared with those from strains that were previously reported in other geographic regions. Since genetic studies on the sampling from the Brazilian surveillance program (BSP) from have not been described, we first established in this paper the comparative phylogenetic among field samples and vaccine strains. Our results showed that all of the Brazilian isolates collected from 2002 to 2005 (n = 39) belongs to the nonvirulent AAvV-1. The substitution of amino acid at position 116 from arginine (R) to alanine (A) (112G-K-Q↓G-A-L117) and at position 114 from glutamine (Q) to threonine (T) (112GR-T↓G-R-L117) in two virus BR-1568 and BR-6225 were observed. Several field isolates were highly similar to the live vaccine strains or strains derived from La Sota or Ulster-type from vaccines currently marketed. The circulation of AAvV-1 strains with different ICPI  induced by vaccine strains  highlights the expanding of the surveillance program is quite necessary, for knowing the characteristics of the virus in the nature, and consequently, to avoid needless risks.

Keywords: Newcastle disease virus, AAvV-1, molecular survey, Brazil.

 

 

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