Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
March 2018 Vol. 7(3): pp. 071-079
Copyright © 2018 Global Advanced Research Journals


Full Length Research Paper

Evolutive phylogenetic based on amino acids sequence surround the fusion protein cleavage site gene of Newcastle disease virus from field samples of surveillance program and vaccine strains in Brazil

Maria Angela Orsi1,2*, Luciana Helena Antoniassi da Silva1, Clarice Weis Arns 1 and Tânia Rosária Pereira Freitas3


1 Animal Virology Laboratory, Institute of Biology, University of Campinas, UNICAMP, Campinas;

2 Animal and Plant Health National Laboratory of  São Paulo, LANAGRO-SP, Campinas, Brazil;

3Animal and Plant Health National Laboratory of Minas Gerais, LANAGRO-MG, 33600-000 Pedro Leopoldo, MG, Brazil.

*Corresponding Author's Email:

Accepted 02 March, 2018



The AAvV-1 official surveillance provided an opportunity for the survey of infections in poultry for internal consume and to exportation, applying molecular methods. In this work, the RT-PCR and direct sequencing of partial fusion protein gene from AAvV-1isolates from poultry production of different Brazilian geographic areas as from live vaccines applied in each region were performed.  The deduced amino acid sequences were inferred and phylogenetically compared with those from strains that were previously reported in other geographic regions. Since genetic studies on the sampling from the Brazilian surveillance program (BSP) from have not been described, we first established in this paper the comparative phylogenetic among field samples and vaccine strains. Our results showed that all of the Brazilian isolates collected from 2002 to 2005 (n = 39) belongs to the nonvirulent AAvV-1. The substitution of amino acid at position 116 from arginine (R) to alanine (A) (112G-K-Q↓G-A-L117) and at position 114 from glutamine (Q) to threonine (T) (112GR-T↓G-R-L117) in two virus BR-1568 and BR-6225 were observed. Several field isolates were highly similar to the live vaccine strains or strains derived from La Sota or Ulster-type from vaccines currently marketed. The circulation of AAvV-1 strains with different ICPI  induced by vaccine strains  highlights the expanding of the surveillance program is quite necessary, for knowing the characteristics of the virus in the nature, and consequently, to avoid needless risks.

Keywords: Newcastle disease virus, AAvV-1, molecular survey, Brazil.





Aldous EW, Mynn JK, Banks J, Alexander DJ (2003).  A molecular epidemiological study of avian paramyxovirus type 1(Newcastle disease virus) isolates by phylogenetic analysis of a partial nucleotide sequence of the fusion protein gene. Avian Pathol. 32: 239-257

Alexander DJ, Bank J, Collins MS Manvell RJ, Frost KM Speidel EC Aldous EW (1999). Antigenic and genetic characterization of Newcastle disease viruses isolated from outbreaks in domestic fowl and turkeys in Great Britain during 1997. Vet. Rec. 145:417-421

Alexander DJ, Campbel G, Manvell RJ, Collins MS, Parsons G, McNulty MS (1992). Characterization of an antigenically unusual virus responsible for two outbreaks of Newcastle disease in the Republic of Ireland in 1990. Vet. Rec. 130:65-68.

Alexander DJ, Manvell RJ, Lowings JP, Frost KM, Collins MS, Russell PH, Smith J E (1997). Antigenic diversity and similarities detected in avian paramyxovirus type 1 (Newcastle disease virus) isolate using monoclonal antibodies. Avian Pathol. 26:399-418.

Alexander DJ, Newcastle disease other avian paramyxovirus, and avian pneumovirus infectious. In: Saif YM., Barnes HJ, Glisson JR, Fadly AM., McDougald LR, Swayne DE.(Eds). Disease of poultry 11th ed. Iowa State University Press, Ames, IA, pp. 63-99.  2003.

Brasil. Ministério da Agricultura, Pecuária e Abastecimento.Secretaria de Defesa Agropecuária (2013). Plano de Contingência para Influenza Aviária e a Doença de Newcastle Versão 1.4, Brasília.

Chambers P, Millar NS, Emmerson PT (1986). Nucleotide sequence of the gene encoding the fusion glycoprotein of Newcastle Disease Virus. J. Gen. Virol 67:2685-2694.

Collins MS, Bashiruddin JB,  Alexander DJ (1993). Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity. Arch Virol.128 (3-4):363-70.

Fernandes CC, Varani AM, Lemos EGM, Miranda VFO, Silva KR, Fernando FS,  Montassier MFS, Montassier, HJ (2014). Molecular and phylogenetic characterization based on the complete genome of a virulent pathotype of Newcastle disease virus isolated in the 1970s in Brazil. Infect. Genet. Evol. 26:160-167.

Ferraz JBS, Felício PE (2010). Production systems. An example from Brazil Meat Science, 84: 238–243.

Gould AR, Kattenbelt JA, Selleck P, Hansson E, Della-Porta A, Westbury, HA (2001).Virulent Newcastle disease in Australia: Molecular epidemiological analysis of viruses isolated prior to and during the outbreaks of 1998-2000. Virus Res. 77 (1):51-60.

Kim LM, King DJ, Curry PE, Suarez DL, Swayne DF, Stallknech DE, Slemons RD, Pedersen JC,  Senne DA, Winker K, Afonso CL (2007). Phylogenetic diversity among low-virulence Newcastle Disease Viruses from waterfowl and shorebirds and comparison of genotype distributions to those of poultry-origin isolates. J. Virol. 81:12641–12653

Kimura MA (1980). Simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol.16: 111-120.

Lancaster JE, Alexander DJ (1975). Newcastle disease: virus and spread. Monograph No. 11, Canada Department of Agriculture, Ottawa.

Leeuw OS,  Koch G,  Hartog L,  Ravenshorst N,  Peeters BP (2005). Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin–neuraminidase protein. J. Gen. Virol. 86:1759–69.

Liu H, Wang Z, Wu Y, Zheng D, Sun C, Bi D, Zuo Y, Xu T (2007). Molecular Epidemiological analysis of Newcastle disease virus isolated in china in 2005. J. Virol. Methods 140 (1-2):206-211.

Mase M, Imai K, Sanada Y, Sanada N, Yuasa N, Imada T, Tsukamoto K, Yamaguchi S (2002). Phylogenetic analysis of Newcastle disease virus genotypes isolated in Japan. J. Clin. Microbiol.40.10:3826–3830

Miller PJ, Kim LM, Afonso CL, Ip HS (2009). Evolutionary dynamics of Newcastle disease virus. Virol 391:64-72.

OIE -World Organization for Animal Health, (2015). In: Manual of Diagnostic Test and Vaccines for Terrestrial Animal , Chapter  2.3.14:555-573.

Orsi MA, Doretto Jr L, Albieri SC, Ribeiro SAM, Yoshida LT (2001). Quality control of live vaccines against Newcastle disease in the period 1993 to 2000. In: Virus Rev. Res. 06(2):126.

Orsi MA, Doretto Jr L, Camillo SCA., Reischak D, Ribeiro SAM, Ramazzoti A, Mendonça AO, Spilki FR, Buzinaro MG, Ferreira HL, Arns CW (2010b). A survey for maintenance of virulent Newcastle disease virus-free area in poultry production in Brazil. Braz. J. Microbiol. 41:368-375

Orsi MA., Doretto Jr L, Camillo SCA., Reischak D, Ribeiro SAM, Ramazzoti A, Mendonça AO, Spilki FR, Buzinaro MG, Ferreira HL, Arns CW (2010a). Prevalence of Newcastle disease virus in broiler chickens (Gallus gallus) in Brazil. Braz.J. Microbiol.41:349-357.

Rout SN, Samal SK (2008). The Large Polymerase Protein Is Associated with the Virulence of Newcastle Disease Virus.  J. Virol. 82, (16) 7828–7836,

Saitou N, Nei M (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.

Samal S, Kumar S, Khattar SK, Samal SK (2011). A single amino acid change, Q114R, in the cleavage-site sequence of Newcastle disease virus fusion protein attenuates viral replication and pathogenicity. J. Gen. Virol  92: 2333–2338

Spann MN, Collins PL, Teng MN.(2003). Genetic Recombination during coinfection of Two Mutants of Human Respiratory  Syncytial Virus.  J. Virol. 77: 11201-11211.

Tamura K, Dudley J, Nei M, Kumar S (2007). MEGA4: Molecular Evolutionary Genetic Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24(8): 1596-1599.

Tan LT, Xu HY, Wang YL, Qin ZM, Sun L, Liu WJ, Cui ZZ (2008). Molecular Characterization of three new virulent Newcastle Disease Virus variants isolated in China.  J. Clin. Microbiol. 46(2): 750-753. 

Van Borm S, Rizotto LS, Ullmann LS,  Scagion GP, Malossi CD, Simão RM,  Araújo Jr JP, Cordeiro IM, Keid  LB, Oliveira TMFS, Soares RM, Martini MC, Orsi MA, Arns CW, Ferreira, HL (2016). Complete genome sequence of a vaccinal Newcastle Disease Virus strain isolated from an Owl (Rhinoptynx clamator). Genome Announcements, 4  (6) e01243-16

Wajid A, Dimitrov KM, Wasim M, Rehmani SF, Basharat A, Bibi T, Arif S, Yaqub T, Tayyab M, Ababneh M, Sharma P, Miller PJ, Afonso CL (2017). Repeated isolation virulent Newcastle disease viruses in poultry and captive non-poultry avian species in Pakistan from 2011 to 2016. Prev. Vet. Med. 142: 1-6

Whemann O, Herczeg J, Tanyi J, Nagy E (1999). Lentogenic field isolates of Newcastle disease virus isolates in Canada and Hungary are identical with the vaccine type used in the region. Avian Pathol.  28: 6-12.



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