Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
March 2019 Vol. 8(3): pp. 090-100
Copyright © 2019 Global Advanced Research Journals

 

Full Length Research Paper

Biosynthesized of zinc oxide nanoparticles using Aspergillus terreus and their application as antitumor and antimicrobial activity 

Dina El-Kahky1, Magdy Attia2, Saadia M Easa1,  Nemat M. Awad2 and Eman A. Helmy3

1Microbiology Department, Faculty of Science, Ain Shams University, cairo, Egypt

2Agricultural Microbiology Department, National Research Centre, 33 El-Bohouth Street, (former El- Tahrir Street) Dokki, Giza, Egypt. Postal Code: 12622

3Microbiology Department, The Regional Center for mycology and Biotechnology, Al-Azhar University, cairo, Egypt

*Corresponding Author's Email: d_elkahkey@yahoo.com

Accepted 09 March, 2019

 

Abstract

This study is refers to identify an easy and eco-friendly way for biosynthesis of  nanoparticles of zinc oxide and that is done by utilizing extracts from Aspergillus terreus as well as trying to identify their effectiveness in antibacterial and antimicrobial activities. A test is performed for zinc oxide nanoparticles on some types of gram positive and gram negative bacteria were tested by disc diffusion method against on Luria-Bertani agar media and the antitumor activity of HCT-116 (colon carcinoma cell) cancer cells. The zinc oxide nanoparticles which is synthesized by Asperagillus terruus were characterized by “UV spectro”, Transmission Electron Microscopy “TEM” showed spherical shape and smooth surfaces with an size of about 110 nm, X-ray Diffraction “XRD”, FTIR spectra of synthesized zinc oxide nanoparticles exhibited prominent peaksat 34440.39 cm-1 (NH stretching), 1639.02 cm-1 (N-H bend primary\ amines), 3230.18 cm-1 (amide), 595.896 cm-1 (O-H hydroxyl group) and 638.323 cm-1 (=C-H). The characterization shape of their synthesis. The best zone of inhibition was determined in the synthesized zinc oxide nanoparticles (9.7 mm) against Staphylococcus aureus (28.3 mm) Escherichia coli, MRSA (13.0 mm) and Pseudomonas aeruginosa (17.7 mm).The result showed that synthesized Zinc Oxide nanoparticles are more antibacterial effect than the standard antibiotic disk, Ampicillin Synthesized Zinc Oxide nanoparticles were found law antitumor activity of HCT-116 cancer cells with a IC50 value of 0.7 µl. It provide an approach investigator can meet the requirement of large and wide scale of industrial production with the advantage of low cost, eco-friendly and reproducible.

Keywords: antibacterial, antitumor, Aspergillus terreus, bacteria, biosynthesis, human pathogenic nanoparticles.

 

 

 

 

 

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