Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
March 2019 Vol. 8(3): pp. 090-100
Copyright © 2019 Global Advanced Research Journals


Full Length Research Paper

Biosynthesized of zinc oxide nanoparticles using Aspergillus terreus and their application as antitumor and antimicrobial activity 

Dina El-Kahky1, Magdy Attia2, Saadia M Easa1,  Nemat M. Awad2 and Eman A. Helmy3

1Microbiology Department, Faculty of Science, Ain Shams University, cairo, Egypt

2Agricultural Microbiology Department, National Research Centre, 33 El-Bohouth Street, (former El- Tahrir Street) Dokki, Giza, Egypt. Postal Code: 12622

3Microbiology Department, The Regional Center for mycology and Biotechnology, Al-Azhar University, cairo, Egypt

*Corresponding Author's Email:

Accepted 09 March, 2019



This study is refers to identify an easy and eco-friendly way for biosynthesis of  nanoparticles of zinc oxide and that is done by utilizing extracts from Aspergillus terreus as well as trying to identify their effectiveness in antibacterial and antimicrobial activities. A test is performed for zinc oxide nanoparticles on some types of gram positive and gram negative bacteria were tested by disc diffusion method against on Luria-Bertani agar media and the antitumor activity of HCT-116 (colon carcinoma cell) cancer cells. The zinc oxide nanoparticles which is synthesized by Asperagillus terruus were characterized by “UV spectro”, Transmission Electron Microscopy “TEM” showed spherical shape and smooth surfaces with an size of about 110 nm, X-ray Diffraction “XRD”, FTIR spectra of synthesized zinc oxide nanoparticles exhibited prominent peaksat 34440.39 cm-1 (NH stretching), 1639.02 cm-1 (N-H bend primary\ amines), 3230.18 cm-1 (amide), 595.896 cm-1 (O-H hydroxyl group) and 638.323 cm-1 (=C-H). The characterization shape of their synthesis. The best zone of inhibition was determined in the synthesized zinc oxide nanoparticles (9.7 mm) against Staphylococcus aureus (28.3 mm) Escherichia coli, MRSA (13.0 mm) and Pseudomonas aeruginosa (17.7 mm).The result showed that synthesized Zinc Oxide nanoparticles are more antibacterial effect than the standard antibiotic disk, Ampicillin Synthesized Zinc Oxide nanoparticles were found law antitumor activity of HCT-116 cancer cells with a IC50 value of 0.7 µl. It provide an approach investigator can meet the requirement of large and wide scale of industrial production with the advantage of low cost, eco-friendly and reproducible.

Keywords: antibacterial, antitumor, Aspergillus terreus, bacteria, biosynthesis, human pathogenic nanoparticles.








Amutha K and Godavari A, (2014). Identification of Aspergillus terreus using 18S rRNA gene sequence, Asian Journal of Biochemical and Pharmaceutical Research 4: 2231-2560.

Basnet LM, Shrestha S, Sapkota S, (2019). Prevalence of wormian bones in drie adult human skulls: an osteo-morphometric study in Nepal. Jan;94(1):101-109.

Caixia W,  Xiaoke H, Yan G, Yinglu J (2015). ZnO Nanoparticles Treatment Induces Apoptosis by increasing Intracellular ROS Levels in LTEP-a-2 Cells BioMed Research International, (9): 432-487,

Chandrasekaran J, Brumin M, Wolf D, Leibman D, Klap C, Pearlsman M (2016). Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology.Mol. Plant Pathol.17 1140–1153

Chang PK, Ehrlich KC (2010). What does genetic diversity of Aspergillus flavus tell us about Aspergillus oryzae? International Journal Food Microbiol 138:189-99.

Ghareib MM, Attia M, Awad N. M, Matter MZ, El-Kahky D (2015). Screening of Fusarium spp Resistance to Silver Ions for Ability to Synthesize Silver Nanoparticles from Egyptian Soils. Global Advanced Research Journals 4(9) pp. 366-382.

Happy A, Venkat S, Kumar S,  kumar R (2017). A review on green synthesis of zinc oxide nanoparticles –An eco-friendly approach Resource-Efficient Technologies 3: 406–413.

Hinrikson HP, Hurst SF,   de Aguirre L,  Morrison CJ (2014). Identification of Aspergillus terreus using 18S rRNA gene sequence, Asian Journal of Biochemical and Pharmaceutical Research 4: 2231-2560.

Jeevan R, Cromwell DA, Trivella M (2012). Reoperation Rates after Breast-Conserving Surgery for Breast Cancer among Women in England: Retrospective Study of Hospital Episode Statistics. BMJ, 345, e4505.

Jesline A, Neetu P, John PM, Narayanan C, Murugan VS (2015). Antimicrobial activity of zinc and titanium dioxide nanoparticles against biofilm-producing methicillin-resistant Staphylococcus aureus Appl Nanosci 5:157–162

Jiang HS, Qiu X N, Li GB, Li W, Yin LY (2014). Silver nanoparticles induced accumulation of reactive oxygen species and alteration of antioxidant systems in the aquatic plant Spirodela polyrhiza. Environ Toxicol Chem 33:1398-1405

Kumar HK, Savalgi VP (2017). Microbial Synthesis of Zinc Nanoparticles Using Fungus Isolated from Rhizosphere Soil. Int. J. Curr. Microbiol. App. Sci. 6(12): 2359-2364.

Kurtzman CP, Smilley M J, Robnett CJ, Wicklow DT (1986). DNA relatedness among wild and domesticated species in the Aspergillus flavus group. Mycologia 78: 955e9.

Navin N, Krasnitz A, Rodgers L, Cook K, Meth J, Kendall J, Riggs M, Eberling Y, Troge J, Grubor V (2010). Inferring tumor progression from genomic heterogeneity. Genome Res. 20, 68–80.

Nei M, Li WH (1979) Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases. Proceedings of the National Academy of Sciences of the United States of America, 76, 5269-5273

Prasanth  LA, Nathaniel K, Rémi M (2015). Fast LSTD using stochastic approximation: Finite-time analysis and application to traffic control. In Joint European Conference on Machine Learning and Knowledge Discovery in Databases, pages 66–81. Springer,

Premanathan M, Karthikeyan K, Jeyasubramanian K, Manivannan G (2011).  Selective toxicity of ZnO nanoparticles toward Gram-positivebacteria and cancer cells by apoptosis through lipid peroxidation. Nanomedicine: Nanotech. Biol. Med.7: 184–192.

Rajan A, Cherian E, Baskar G (2016). Biosynthesis of zinc oxide nanoparticles using Aspergillus fumigatus JCF and its antibacterial activity International Journal of Modern Science and Technology  2: 52-57.

Sammons DW, Adams LD, Nishizawa EE (1981). Ultrasensitive silver-basedcolour staining of polypeptides in polyacrylamide gels. Electrophoresis, 2:135-141.

Sangappa M, Vandana SP, Bharath AU, Thiagarajan P (2013). Mycobiosynthesis of novel nontoxic zinc oxide nanoparticles by a new soil fungus Aspergillus terreus VIT Journal of Chemical and Pharmaceutical Research, 5(12):1155-1161

Sharmila Bhanu P, Devi Sankar K, Shanthi V, Sujatha K, Subhadra DV (2017). Evaluation of biochemical and immunohistochemical placental antioxidant status in normal and gestational diabetes mellitus mothers International Journal of Anatomy and Research, Vol 5(3.1):4082-89.

Sneath PHA, Sokal RR (1973). Numerical Taxonomy: The Principles and Pratice of Numerical Classification Freeman.(7): 418-573.

Vijayakumar S, Malaikozhundan B, Shanthi S (2018).Control of biofilm-forming clinically important bacteria by green synthesized ZnO nanoparticles and its ecotoxicity on Ceriodaphnia cornuta. Microb Pathogenesis , 107:88–97.

Williams DB, (1996). Transmission Electron Microscopy. Material Science, Plenum Press,4:571-625.

Zhang Y, Yan L, Xu W, Guo X, Cui L, Gao L, Wei Q, Du B (2014). Adsorption of Pb (II) and Hg (II) from aqueous solution using magnetic CoFe2O4 reduced graphene oxide. Journal Mol. Liq. 191:177–182.









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