Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
March 2019 Vol. 8(3): pp. 101-108
Copyright © 2019 Global Advanced Research Journals
Full Length Research Paper
Principal Component Analysis of Indigenous Lentil (Lens culinaris) Germplasm Collection
Nadiya A. Al-Saady1,, Saleem K. Nadaf*1, Ali H. Al-Lawati1 and Saleh A. Al-Hinai2,
1Oman Animal & Plant Genetics Resources Center, The Research Council, PO Box 92, PC 123, Sultan Qaboos University Campus, Sultanate of Oman.
2Directorate General of Agriculture & Livestock Research, Ministry of Agriculture, PO Box 50 PC 121, Al-Seeb, Sultanate of Oman
*Corresponding Author's Email: firstname.lastname@example.org
Accepted 15 March, 2019
This paper presents the results of collecting missions undertaken from 2008 to 2011 and assessment of seed diversity of indigenous lentil (Lens culinaris) germplasm in Oman. 11 seed samples/accessions of lentil were collected of which Al-Dhahira & Buraimi governorates had the highest (6), followed by South Batinah-coastal governorate represented by mountains of Rustaq (3) and Al-Dakhliya (Interior) governorate (2) with no contribution from other agriculturally important Sharqiya (eastern), North Batinah and Southern Dhofar governorates. Seed accessions were diverse with respect to four seed traits investigated like seed length (cm) and width (cm), 1000-seed weight (g) and seed color. Seed length varied from 0.33 cm to 0.45 cm; seed width ranged from 0.320 cm to 0.405 cm and 1000-seed weight ranged from 22.5 g to 32.5 g. With respect to seed color, the accessions were classified into five groups based on simple to complexity of color combinations with scores from 1-5 with varying frequencies / numbers. These accessions were grouped into 4 genetically diverse clusters corresponding to their spread in four quadrants of the biplot graph based on the Principal Component Analysis (PCA) using seed traits which showed substantial contribution of seed length (38.095 %) followed by seed width (32.930%) and 1000-seed weight (28.733%), with the least contribution by seed color (0.242%) to the first principal component (PC1 or factor 1). Of the six-character combinations, only two correlation coefficients viz. between seed length and seed width (0.864*) and between seed length and seed weight (0.710*), were significant (p<0.05).
Keywords: Diversity, collections, seed characters, seed color, lentil.
Ahamad KU, Akhter B, Islam MA, Humayun MR, Alam MJ (2014). Morphological characterization and genetic diversity in Lentil (Lens culinaris Medikus ssp.culinaris) germplasm. Int. J. Agril. Res. Innov. & Tech. 4: 70-76.
Alam AKMM, Podder R, Sarker A (2011). Estimation of genetic diversity in lentil germplasm. J. Agril. Sci. Agrivita. 33: 103-110.
Al-Maamari IT, Al-Sadi AM, AlSaady NA (2014). Assessment of genetic diversity in fenugreek (Trigonella foenum-graecum) in Oman. Int. J. Agri. Biol. 16: 813-818.
AlSaady NA, Nadaf SK, Al-Hinai SA, Al-Subhi AS, Al-Farsi SM, Al-Habsi KM, Esechie HA, Siddique KHM (2014). Multicrop Legume Germplasm Collection in Oman. Int. J. Agri. Biol. 16: 231-241.
AlSaady NA, Nadaf SK, Al-Hinai SA, Al-Subhi AS, Al-Farsi SM, Al-Habsi KM, Esechie HA, Siddique KHM (2018a). Fenugreek (Trigonella foenum- graecum L.) germplasm collection in Oman. Int. J. Agri. Innovations and Res. (IJAIR). 6: 212-217.
AlSaady NA, Nadaf SK, Al-Hinai SA, Al-Subhi AS, Al-Farsi SM, Al-Habsi KM, Esechie HA, Siddique KHM (2018b). Germplasm collection in Alfalfa (Medicago sativa L.) in Oman. Int. J. Agri. Innovations and Res. (IJAIR). 6: 218-224.
Bicer BT (2009). The effect of seed size on yield and yield components on chickpea and lentil. African J, Biotech. 8: 1482-1487.
Dijkstra H, Soest LJM (1986). Descriptor list Pulses; lupines, peas and faba beans. CGN, Wageningen. 8p.
Erdogan C (2015). Genetic characterization and cotyledon color in lentil. Chilean J. Agric. Res. 75(4). ISSN 0718-5839. http://dx.doi.org/10.4067/S0718-58392015000500001
FAO (2016). FAO statistical yearbook. Food and Agriculture Organization (FAO) of the United Nations, Rome, Italy. http:// www. fao.org/faostat/en/#data/QC/ visualize.
Gaad D, Laouar M, Abdelguerfi A (2018). Collection and ethnobotanical investigation of lentil (Lens culinaris Medik) in Algeria. Recent Res. Sci. Tech. 10:1-6. ISSN: 2076-5061.
Ghassemi-Golezoni K, Chadordroz-Jeddi A, Zahtab-Salmasi S (2014). Effects of seed size and aging on field performance of lentil (Lens culinaris M.) under different irrigation treatments. Acta Agriculture, Slovencia. 103: 158-166.
Guarino L (1990). Crop collecting in the Sultanate of Oman in the context of Arabian Peninsula. FAO/ IBPGR. PGR Newsl. 77: 27-33. Gupta D. Taylor PWJ, Inder P, Phan HTT, Ellwood SR, Mathur PN, Sarker A, Ford AR (2012). Integration of EST–SSR markers of Medicago truncatula into intraspecific linkage map of lentil and identification of QTL conferring resistance to Ascochyta blight at seedling and pod stages. Mol. Breed. 30: 429-439.
Hamdi A, Erskine W, Gates P (1991). Relations among economic characters in lentil. Euphytica. 57: 109-116.
Hay FR, Probert RJ (2011). Collecting and Handling in the Field. Collecting plant genetic diversity: Technical guidelines 2011 update. Crop Gene Bank. http: // www. Cropgenebank.sprp.cgiar. org/index, 2011.
Hodgkin T (1997). Some current issues in conservation of genetic resources. In: Ayad WG, Hodgkin T, Jaradat, A, Rao VR (Eds). (1995). Molecular genetic techniques for plant genetic resources. Report of an IPGRI workshop. 9-11 October 1995, Rome, Italy.
IPGRI (1995). Collecting Plant Diversity: Technical Guidelines. In L. Guarino, VR Rao, R Read (Eds.) CABI.
MAF (2005). Agriculture Census-2004-2005. Ministry of Agriculture & Fisheries. Sultanate of Oman.
MAF (2017). Annual Agriculture Statistics-2017. Director General of planning and Investment Promotions. Department of Statistics and Information. Ministry of Agriculture & Fisheries. Oman.
Mathur VL (2010). Genetic divergence in fenugreek (Trigonella foenum-graecum L.). Indian J. Genet. & Plant Breed. 52, 2010, 428-432.
Mehra R, Sarkar A, Khandia R, Munjal A (2018). Genetic variability for quantitative traits in Lentil (Lens culinaris). Int. j. Adv. Sci. Eng. & Tech. 6: 11-19.
Noor R, Khan Sm, Ahmed E, Hussain M, Abdallah EF, Alqarawi AA, Hashem A, Aldubise A. (2017). The morpho-agronomic characterization of Lens culinaris germplasm under salt marsh habitat in Swat, Pakistan. Saudi J. Biol. Sci. 24: 1639-1645.
Singh A, Sharma VK, Dikshit HK, Singh D, Aski M, Prakash P, Kaushik SC, Singh G, Kumar S, Sarker A (2017). Microsatellite marker-based genetic diversity analysis of elite lentil lines differing in grain iron and zinc concentration. J. Plant Biochem. Biotech. 26: 199-207.
Solh M, Erskine W (1984). Genetic Resources in lentils, In: J.R. Witcombe and W. Erskine (eds.) Genetic Resources and Their Exploitation- Chickpeas, Faba beans and Lentils. ISBN-13:978-94-009-613l-3(PB). Dr W. Junk Publishers for ICARDA and IBPGR. 205-224.
Toklu F, Biçer BT, Karaköy T (2009). Agro- morphological characterization of the Turkish lentil landraces. African J. Biotech. 8 (17): 4121-4127.
Turk MA, Tawaha AM, Lee KD. 2004. Seed germination and seedling growth of three lentil cultivars under moisture stress. Asian J. Plant Sci. 3: 394-397.
XLStat. 2017. Data Analysis and Statistical Solution for Microsoft Excel. Addinsoft, Paris, France. 2017.
Younis N, Hanif M, Sadiq S, Abbas G, Asghar, MJ (2008). Estimates of genetic parameters and path analysis in lentil (Lens culinaris Medik). Pak. J. Agric. Sci. 45: 44-48.
Zohary D, Hoph M (1973). Domestication of pulses in the old world. Science. 182: 887-894.
- Nadiya A. Al-Saady on Google Scholar
- Nadiya A. Al-Saady on Pubmed
- Saleem K. Nadaf on Google Scholar
- Saleem K. Nadaf on Pubmed
- Ali H. Al-Lawati on Google Scholar
- Ali H. Al-Lawati on Pubmed
- Saleh A. Al-Hinai on Google Scholar
- Saleh A. Al-Hinai on Pubmed