Global Advanced Research Journal of Agricultural Science (ISSN: 2315-5094) 

April 2012 Vol. 1(2), pp 033-047

Copyright © 2012 Global Advanced Research Journals

Full Length Research Paper

Isolation of fungi causing rot of cocoyam (colocasia esculenta (l.) schott) and control with plant extracts: (allium sativum, l., garcinia kola, heckel., azadirachta indica, l. and carica papaya, l.) 

Anukworji C A1*, Ramesh .R.Putheti2*,R. N. Okigbo3*

1,3*Department of Botany,Nnamdi Azikiwe University, Awka,Anambra State, Nigeria.2* Member,American Association of Pharmaceutical Sciences,USA. 

Corresponding Author E-mail: rutwikusa@yahoo.com 

Accepted 02 April, 2012

                                                                                                                           Abstract

Antifungal effect of aqueous and ethanol extracts of Allium sativum, Azadirachta indica, Carica papaya and Garcinia kola on the growth of fungal pathogen of stored cocoyam cormels were investigated invitro. Four different extract concentrations were obtained from each plant parts used by blending 25g, 50g, 75g and 100g in 100ml of sterile distilled water (SDW). Phytochemical screening of the plants was conducted using different standard methods; this revealed the presence of alkaloid, saponin, tannins, flavonoid, phytate, oxalate and phenol in all the plants but at different concentrations. Effect of standard antibiotics (Grisovid) comparative to the plant extracts was determined. Pathogenicity test revealed that Aspergillus niger, Fusarium solani, Sclerotia rolfsii  and Botryodiplodia theobromae induced rot in healthy cocoyam cormels after 6 days of inoculation with Botryodiplodia theobromae being the most virulent. Even though all the extracts showed varying degrees of antifungal efficacy, ethanol extract proved to be more potent. The efficacy of the extract varied with the solvent of extraction, extract concentration and the test pathogens. Inhibition of fungal growth increased with a corresponding increase in extract concentration. Allium sativum and Azadirachta indica depicted an effective/high rate of inhibition on the mycelia growth of all the test fungi ranging from 40.57% to 92.40% whereas, extract of Carica papaya and Garcinia kola showed a lower inhibition rate ranging from 0.0% to 60.17%. 10% extract concentration of Allium sativum and Azadirachta indica being the most fungitoxic showed a significant (P<0.05) inhibition on all the test fungi pathogens. The fungitoxic potential of these plant extracts on rot inducing fungi of stored cocoyam corms/cormels recommends their use to farmers as alternative to commercial / synthetic fungicides. 

Keywords: Fungi, Isolation, Antifungal effect, Cocoyam.

Related Articles


Original Research Articles
Emadeldin Hassan E. Konozy
Isolation, Purification and Characterization of a Thermostable β-Galactosidase isoform from Erythrina indica Seeds
Glo. Adv. Res. J. Agric. Sci. June 2016 Vol: 5(6): - [Abstract] [Full Text - PDF] (963 KB)
Abubakar, A, Nura, M, Auyo, M, I, Sunday, E and Kutama, A. S
A Study on the Population and Types of Fungiin "Mbuli", a Local Drink in Borno State, Nigeria
Glo. Adv. Res. J. Agric. Sci. July 2016 Vol: 5(7): - [Abstract] [Full Text - PDF] (2,375 KB)
Oranusi S, Braide W, Oguoma OI.
Antifungal properties of lactic acid bacteria isolated from Ricinus communis Pentaclethra macrophylla and Yoghurts
Glo. Adv. Res. J. Agric. Sci. February 2013 Vol: 2(1): - [Abstract] [Full Text - PDF] (176 KB)
Tinatin Doolotkeldieva, Saykal Bobusheva
Endophytic fungi diversity of wild terrestrial plants in Kyrgyzstan
Glo. Adv. Res. J. Agric. Sci. December 2014 Vol: 3(10): - [Abstract] [Full Text - PDF] (1,604 KB)
Andy Ramu and Veluchamy Vijayakumar
Antidiabetic activity of 7-methoxycoumarin from the bark of Marine plant Rhizophora mucronata
Glo. Adv. Res. J. Agric. Sci. May 2016 Vol: 4(1): - [Abstract] [Full Text - PDF] (613 KB)
Nura, M, Abubakar, A, Auyo, M, I, Sunday, E and Kutama, A. S
Isolation and Identification of Fungi Associated With Tigernut Milk Drink (Kunun Aya) In Dutse, Jigawa State
Glo. Adv. Res. J. Agric. Sci. July 2016 Vol: 5(7): - [Abstract] [Full Text - PDF] (153 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Anukworji C A on Google Scholar
Anukworji C A on Pubmed
Ramesh R on Google Scholar
Ramesh R on Pubmed
Putheti on Google Scholar
Putheti on Pubmed
RN Okigbo on Google Scholar
RN Okigbo on Pubmed

Statistics

Viewed 863
Printed 286
Downloaded 2267
Powered By iPortal Works