Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
April 2017 Vol. 6(4): pp. 097-107
Copyright © 2017 Global Advanced Research Journals

 

Full Length Research Paper

Factors Affecting Agrobacterium-mediated Transformation and Callus  Induction of Sudan’s Cotton Genotypes

Iman K.A. Abdel Gadir 1,2, Marmar A. El Siddig3*, Hayfa H.A. Ibrahim3 and Adil A. El Hussein3

 

1Faculty of Science, Jazan University, Saudi Arabia;

2Faculty of Science and Technology, Shendi University, Sudan;

3Faculty of Science, University of Khartoum, Sudan

Email: marmarelsiddig@hotmail.com

Accepted 10 April, 2017

 

Abstract

The objective of this study was to optimize conditions affecting transformation of insect resistance gene (cry 1Ac) into locally grown cotton cultivars (Hamid, Barakat- 90) using a binary Agrobacterium tumefaciens vector. The highest percentages (99.33 and 85.67%) of callus induction from hypocotyls of Hamid and Barakat- 90, respectively were observed in MS medium supplemented with 2 mg/L 2-isopentynyl adenine (2iP) + 2 mg/ L IAA. Transformation/callus induction protocol for cotton cultivars were developed using A. tumefaciens EHA105 strain harboring the pCAMBIA 2301 vector which contains a cry 1Ac gene coding for insecticide resistance under the transcriptional control of cauliflower mosaic virus 35S promoter. Results showed that 16.1—22.3% of the co-cultivated explants were Hygromycin resistant and were considered as putative transformants. Transformation was further confirmed by GUS histochemical assay and PCR amplification of the 35S promoter, Nos terminator, Hygromycin phosphotransferase (hpt) and Kanamycin resistance (nptII) genes which were found on the inserted construct. GUS expression was observed in transformed cotton calli but never in the non-transformants (control). Also, transformed calli gave the target PCR amplicons for all of the examined genes while the untransformed tissues didn’t. Transformation percentages of 16.7 for Hamid and 11.9 for Barakat- 90 were reported. 

Keywords: Transformation, callus, cotton, Agrobacterium tumefaciens.


Related Articles


Systematic Review
Fernando Romero Torres, Augusto Renato Pérez Mayo and Humberto García Jiménez
Subsidies to the agricultural sector for the search for food sovereignty. A funding option for a strategic sector of the national economy. Case: Mexico
Glo. Adv. Res. J. Agric. Sci. May 2018 Vol: 7(5): - [Abstract] [Full Text - PDF] (243 KB)
Original Research Articles
Jiangning Zhao
A cognitive discussion on Shanzhai An emerging innovation model
Glo. Adv. Res. J. Agric. Sci. March 2013 Vol: 2(3): - [Abstract] [Full Text - PDF] (321 KB)
Kutama, A.S, Sherif, U, Dangora I.I, Umma, M, and Salusu A., Rabiu Musa Kwankwaso
Yield of Cotton and the Opinion of Small-Scale Farmers on Cotton Production in Kano and Katsina States, Nigeria
Glo. Adv. Res. J. Agric. Sci. August 2015 Vol: 4(8): - [Abstract] [Full Text - PDF] (116 KB)
Soulamaa S, Atcholia KE, Naonb B, Almusawia A, Bedac T and Gomesa S
Processing of a bio sourced material and determination of its properties compound of recycled polymer reinforced with vegetable stocks from non food biomass residues
Glo. Adv. Res. J. Agric. Sci. August 2014 Vol: 3(6): - [Abstract] [Full Text - PDF] (1,161 KB)
Review
Arshid Ahmad Khanday and H.S. Dwivedi
Phytotoxicity of organophosphates with particular reference to triazophos in the agriculture of India- A review
Glo. Adv. Res. J. Agric. Sci. October 2018 Vol: 7(7): - [Abstract] [Full Text - PDF] (156 KB)
Original Research Article
Gado ND
A Multi Discriminate Analysis 0f Performance Inducing Variables A Case of the Textile Industry in North West Nigeria 1989 to 2010
Glo. Adv. Res. J. Agric. Sci. August 2013 Vol: 2(8): - [Abstract] [Full Text - PDF] (100 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Iman K.A. Abdel Gadir on Google Scholar
Iman K.A. Abdel Gadir on Pubmed
Marmar A. El Siddig on Google Scholar
Marmar A. El Siddig on Pubmed
Hayfa H.A. Ibrahim on Google Scholar
Hayfa H.A. Ibrahim on Pubmed
Adil A. El Hussein on Google Scholar
Adil A. El Hussein on Pubmed

Statistics

Viewed 1025
Printed 214
Downloaded 517
Powered By iPortal Works