Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
April 2018 Vol. 7(4): pp. 125-129
Copyright © 2018 Global Advanced Research Journals

 

Full Length Research Paper

Establishment of an Efficient in Vitro Regeneration Protocol for Mass Propagation via organogenesis of Endangered Plant Rhanterium epapposum in Qatar

Maha T. AL Henzab, Amira O. Elbadawi and Fatima H. Ibrahim, Rehab Sidky*

 

Agriculture Research Department, Ministry of Municipality and Environment. Qatar

*Corresponding Author's Email: rasedky@mme.gov.qa

Accepted 03 April, 2018

 

Abstract

Wild plants make an important contribution to the life of local communities. Endangered plant species like Rhanterium epapposum olive (Arfaj) were micropropogated through tissue culture technique in order to protect them from extinction and conserving Biodiversity in Qatar. Agriculture Research Department (ARD) has developed an efficient protocol for rapid and large-scale in vitro propagation of the native annual plants Rhanterium epapposum that can not be propagated on a large-scale by means of seeds and cuttings. Apical bud and nods were collected from wild habitat and cultured on modified Murashige and Skoog (MS) medium supplemented with1.5mg l-1 BA (initiation stage) and containing 1mg l-1 BAP+ 0.2 mg l-1 Kinetin for multiplication stage. A continuous of mass propagation was achieved through three times sub-culture of explants with regular transferring to fresh medium containing the same composition. For rooting stage, elongated shoots were cultured on different strength of Murashige and Skoog media (full and half) supplemented with different auxin. Full MS nutrient medium containing 1 mg l-1 IBA increased significantly the number of roots and root length compared with other treatments. The plantlets were adapted in greenhouse conditions successfully. The optimized protocol proposed here can be used to propagate the plants and conserve it from extinction.

Keywords: In Vitro, Plant, Rhanterium epapposum, organogenesis.

 

 

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