Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
April 2018 Vol. 7(4): pp. 104-109
Copyright © 2018 Global Advanced Research Journals

 

Full Length Research Paper

Wild Olive Leaf Extracts Up regulate Insulin-signaling Gene Expression in Diabetic mice

1Afaf S. Altalhi; 1&2**Aziza M. Hassan; 3Shatha Dhaifallah Al-Harthi and 3Fatima Sulaiman  Al-harbi

 

1Biotechnology Department, Faculty of Science-Taif University, Taif-KSA

2 Cell Biology Department, National Research Center, Dokki, Giza- Egypt

3Biology Department, Faculty of Science-Taif University, Taif-KSA

*Corresponding Author's Email: Dr_azizahassan@yahoo.com

Accepted 31 December, 2017

 

Abstract

Regulation of insulin gene expression in response to increases in blood glucose levels is essential for maintaining normal glucose homeostasis; however, the exact mechanisms by which glucose stimulates insulin gene transcription are unclear. Olive leaves extracts(OLE) are reported to have beneficial effects on people with normal and impaired glucose tolerance, the metabolic syndrome, type 2 diabetes, and insulin resistance. However, clinical results are controversial and the molecular characterization of OLE effects is limited. This study investigated the effects of OLE, prepared from wild olive leaves, on gene expression of insulin and glucagon in mice tissues. Diabetes in mice was induced by intraperitoneal injections of alloxan. The alterations in gene expressions of pancreatic insulin and hepatic glucagon were evaluated throughout the RT-PCR analysis. The results indicated that diabetic mice showed significantly increased in the expression of pancreatic insulin gene followed by significantly decreased in the expression of hepatic glucagon gene. The administration of OLE at 0.33 g/kg for six days before alloxan treatment; up-regulated pancreatic insulin and modulated hepatic glucagon genes expression. Meanwhile, administration of OLE after induced diabetic mice showed a highly significant increase in gene expression of pancreatic insulin followed by significantly decreased in gene expression of hepatic glucagon. Results suggest that the OLE possess a potent anti-hyperglycemic effect, which may be due to the presence of antioxidants such as polyphenols, these antioxidant activities restraining the oxidative stress which is widely associated with diabetes pathologies and complications.

Keywords: Olive leaves, Insulin, Glucagon, Diabetes, Gene expression.

 

 

REFERENCES

 

Adiga S, Bairy KL, Meharban A, Punita IS (2010): Hypoglyce­mic effect of aqueous extract of Trichosanthesdioica in normal and diabetic rats. Int J Diabetes Dev Ctries; 30:38-42.

Bock M, Jose´ G, Derraik B, Christine M, Brennan, Janene B, Biggs, Philip E, Morgan, Steven C, Hodgkinson, Paul L, Hofman, Wayne S, Cutfield (2013): Olive (Oleaeuropaea L.) Leaf Polyphenols Improve Insulin Sensitivity in Middle-Aged Overweight Men: A Randomized, Placebo-Controlled, Crossover Trial. plosone.org vol.8 (3), e57622, 1 – 8

Brun ME, Gasca S, Girard C, Bouton K, De Massy B, De Sario A (2006). Characterization and expression analysis during embryo development of the mouse ortholog of MLL3, Gene 371: 25–33.

Chiang CK, Ho TI, Peng YS, Hsu SP, Pai MF, Yang SY, Hung KY, Wu KD (2007): Rosiglitazone in diabetes control in hemodialysis patients with and without viral hepatitis infection: effectiveness and side effects. Diabetes Care; 30: 3-7.

Cvjeti-anin T, Miljkovi D, Stojanovi I, Dekanski D, Stoši-Gujii S (2010): Dried leaf extract of Oleaeuropaea ameliorates islet-directed autoimmunity in mice. Br J Nutr; 103: 1413-24.

Dixon RA, Xie DY, Sharma SB (2005). Proanthocyanidins—a final frontier in flavonoid research? New  Phytol. 165, 9–28.

Dragana D, Slavica R, Nevena VR, Nataša DP, Aleksandar D, Dušan MM (2011): Olive leaf extract modulates cold restraint stress-induced oxidative changes in rat liver J. Serb. Chem. Soc. 76 (9) 1207– 1218.

Eriko K, Shinya Y, Isafumi M, Mitsuhiro K, Kazuaki K, Yasuhiro O, Yoji T (2003): Identification of Anti-α-Amylase Components from Olive Leaf Extracts Food Science and Technology Research; Vol. 9 , No. 1 pp.35-39.

Fu Z, Gilbert ER, Liu D (2013). Regulation of insulin synthesis and secretion and pancreatic Beta-cell dysfunction in diabetes. Curr Diabetes Rev. 2013 Jan 1;9(1):25-53. Review.

Gupta S, Sharma SB, Bansal SK, Prabhu KM (2009): Antihyper­glycemic and hypolipidemic activity of aqueous extract of Cassia auriculata L. leaves in experimental diabetes. J Ethnopharmacol; 123: 499-503.

Hamden K, Carreau S, Boujbiha MA, Lajmi S, Aloulou D, Kchaou D, El feki A (2008): Hyperglycaemia, stress oxidant, liver dysfunction and histological changes in diabetic male rat pancreas and liver: Protective effect of 17 β- estradiol. Steroids, 73, 495–501.

Hamza N, Berke B, Cheze C, Agli AN, Robinson P, Gin H, Moore N (2010): Prevention of type 2 diabetes induced by high fat diet in the C57BL/6J mouse by two medicinal plants used in traditional treatment of diabetes in the east of Al­geria. J Ethnopharmacol; 128:513-518.

Hassan AM, Abdel-Aziem SH, Abdel-Wahhab MA (2012): Modulation of DNA damage and alteration of gene expression during aflatoxicosis via dietary supplementation of Spirulina (Arthrospira) and whey protein concentrate. Ecotoxicol Environ Saf 79:294–300

Jänicke J, Grünwald, Brendler T (2003): Handbuch Phytotherapie, Wissenschaftliche Verlagsgesellschaft, Stuttgart, Germany.

Jemai H, El Feki A, Sayadi S (2009): Antidiabetic and antioxidant effects of hydroxytyrosol and oleuropein from olive leaves in alloxan-diabetic rats. J Agric Food Chem; 57: 8798-804.

Jouad H, Haloui M, Rhiouani H, El Hilaly J, Eddouks M (2001): Ethnobotanical survey of medicinal plants used for the treatment of diabetes, cardiac and renal diseases in the North Center Region of Morocco (fez-Boulemane). J. Ethnopharmacol, 77, 175–182.

Karakaya S (2009): Olive tree (Oleaeuropaea) leaves: potential beneficial effects on human health. Nutr Rev; 67:632–638.

Khodabandehloo H,  Gorgani-Firuzjaee S, Panahi G,  Meshkani R (2016). Molecular and cellular mechanisms linking inflammation to insulin resistance and β-cell dysfunction. Translational Research Volume 167, Issue 1, January 2016, Pages 228-256

Kim YM, Namkoong S, Yun YG, Hong HD, Lee YC, Ha KS, Lee H, Kwon HJ, Kwon YG, Kim YM. 2007: Water extract of Korean red ginseng stimulates angiogenesis by activating the PI3K/Akt-dependent ERK1/2 and eNOS pathways in human umbilical vein endothelial cells. Biol Pharm Bull; 30:1674-1679.

Liao Z, Chen X, Wu M (2015): Antidiabetic effect of flavones from Cirsiumjaponicum DC in diabetic rats. Arch Pharm Res; 33:353-362.

Matough FA; Budin SB, Hamid ZA, Alwahaibi N,  Mohamed J (2012). The Role of Oxidative Stress and Antioxidants in Diabetic Complications. Sultan Qaboos Univ Med J. 2012 Feb; 12(1): 5–18

Miyazaki Y, Matsuda M, DeFronzo RA (2002): Dose-response effect of pioglitazone on insulin sensitivity and insulin secretion in type 2 diabetes. Diabetes Care 25: 517–523.

Nukatsuka M, Sakurai H, Kawada J (1989): Generation of alloxan free radicals in chemical and biological systems: implication in the diabetogenic action of alloxan. Biochem. Biophys. Res. Commun. 165, 278|283

Poudyal H, Campbell F, Brown L (2010): Olive leaf extract attenuates cardiac, hepatic, and metabolic changes in carbohydrate-, high fat-fed rats. J Nutr 140: 946-53.

Prasadan K, Daume E, Preuett B, Spilde T, Bhatia A,  Kobayashi  H, Hembree, M, Manna P, Gittes GK (2002): Glucagon Is Required for Early Insulin-Positive Differentiation in the Developing Mouse Pancreas. Diabetesvol. 51 no. 11 3229-3236

Prior RL, Gu L (2005). Occurrence and biological significance of roanthocyanidins in the American diet. Phytochemistry 66, 2264–2280.

Ryu JK, Lee T, Kim DJ, Park IS, Yoon SM, Lee HS, Song SU, Suh JK (2005): Free radical-scavenging activity of Korean red ginseng for erectile dysfunction in non-insulin-depen­dent diabetes mellitus rats. Urology; 65: 611-615.

Sambrook J, Fritsch EF, Maniatis T (1989). Molecular Cloning. A Laboratory. Cold Spring Harbor Laboratory Press, New York.

Sanarya T, Al-Badri, Imad M, Al-Ani, Karim A, Al-Jashamy (2011): The Effect of Aqueous Olive Leaves Extract on the Pancreatic Islets of Streptozotocin Induced Diabetes Mellitus in Mice. ANNALS OF MICROSCOPY Vol 11,  4 – 11

Sangi SMA, Sulaiman MI, Abd El-wahab MF, Ahmedani EI, Ali SS (2015). Antihyperglycemic effect of thymoquinone and oleuropein, on streptozotocin-induced diabetes mellitus in experimental animals. Pharmacogn Mag. 2015 Oct; 11(Suppl 2): S251–S257

Sharma R,  Amin H,  Pradeep G,  Prajapati G (2015). Antidiabetic claims of Tinospora cordifolia (Willd.) Miers: critical appraisal and role in therapy. Asian Pac J Trop Biomed, 5(1): 68-78.

Stades AM, Heikens JT, Erkelens DW, Holleman F, Hoekstra JB (2004): Metformin and lactic acidosis: cause or coincidence? A review of case reports. J Intern Med; 255: 179-187.

Tuttle KR, McGill JB, Haney DJ, Lin TE, Anderson PW, PKC-DRS, PKC-DMES, PKC-DRS (2007): Kidney outcomes in long-term studies of ruboxistaurin for diabetic eye disease. Clin J Am Soc. Nephrol; 2:631-636.

Waller RA, Duncan DB (1969): A Bayes rule for the symmetric multiple comparison problems, J. Am. Stat. Assoc. 64 1484–1503.

Wolffram S, Blo¨ck, M, Ader P (2002): Quercetin-3-glucoside is transported by the glucose carrier SGLT1 across the brush border membrane of rat small intestine. J Nutr; 132: 630–635.

Zari TA, Al-Attar AM (2011): “Therapeutic effects of olive leaves extract on rats treated with a sublethal concentration of carbendazim,” European Review for Medical and Pharmacological Sciences, vol. 15, no. 4, pp. 413–426.

Zhu CF, Peng HB, Liu GQ, Zhang F,  Li Y (2010): Beneficial ef­fects of oligopeptides from marine salmon skin in a rat model of type 2 diabetes. Nutrition; 26:1014-1020.

 

Related Articles


Original Research Articles
Karuna Priyachitra, Kantipudi Teja Sri, Cheeli Ramesh, Srinath Nissankararao and Ramadevi Bhimavarapu
In-vitro osmotic fragility assessment of Trigonella foenum graecum linn. seed extract on sheep erythrocytes
Glo. Adv. Res. J. Agric. Sci. July 2015 Vol: 4(7): - [Abstract] [Full Text - PDF] (482 KB)
Cyrille Bilé Ehounoud, Alpha Kabinet Keita, Abdoul Habib Béavogui, Ansoumane Cissé, Nadia Amanzougaghene, Jean David N’Guessan, Didier Raoult, Oleg Mediannikov, Florence Fenollar
Molecular identification of microorganisms in chronic wounds, Republic of Guinea (Conakry)
Glo. Adv. Res. J. Agric. Sci. February 2018 Vol: 7(1): - [Abstract] [Full Text - PDF] (1,118 KB)
Case Report
Wejdan Mohammad Baabdullah, Doha Malik Bin-Talib, Estabrq Al Hachim, Ayman M Oraif and Hassan SO Abduljabbar
Maternal Morbid Obesity Complications. A Case Report
Glo. Adv. Res. J. Agric. Sci. September 2017 Vol: 6(9): - [Abstract] [Full Text - PDF] (747 KB)
Original Research Articles
Halil Onder, GökcemYıldız F and Çagrı Mesut Temucin
Neuromyositis: Clinical and Electrophysiological study of 8 cases
Glo. Adv. Res. J. Agric. Sci. August 2015 Vol: 4(8): - [Abstract] [Full Text - PDF] (96 KB)
Awe JAA, Ugwi V and Omon EH
Inguinal hernias: analysis of incidence, diagnosis and management of 172 consecutive adult cases at Igbinedion university teaching hospital Okada
Glo. Adv. Res. J. Agric. Sci. July 2014 Vol: 3(7): - [Abstract] [Full Text - PDF] (366 KB)
Rajech Sharkia, Muhammad Mahajnah, Ahmad Sheikh-Muhammad, Mohammad Khatib and Abdelnaser Zalan
Trends in the prevalence of type 2 diabetes mellitus among Arabs in Israel: A community health survey
Glo. Adv. Res. J. Agric. Sci. April 2018 Vol: 7(4): - [Abstract] [Full Text - PDF] (139 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Afaf S. Altalhi; Aziza M. Hassan; Shatha Dhaifallah Al-Harthi on Google Scholar
Afaf S. Altalhi; Aziza M. Hassan; Shatha Dhaifallah Al-Harthi on Pubmed
Fatima Sulaiman Al-harbi on Google Scholar
Fatima Sulaiman Al-harbi on Pubmed

Statistics

Viewed 330
Printed 95
Downloaded 322
Powered By iPortal Works