Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
April 2018 Vol. 7(4): pp. 104-109
Copyright © 2018 Global Advanced Research Journals


Full Length Research Paper

Wild Olive Leaf Extracts Up regulate Insulin-signaling Gene Expression in Diabetic mice

1Afaf S. Altalhi; 1&2**Aziza M. Hassan; 3Shatha Dhaifallah Al-Harthi and 3Fatima Sulaiman  Al-harbi


1Biotechnology Department, Faculty of Science-Taif University, Taif-KSA

2 Cell Biology Department, National Research Center, Dokki, Giza- Egypt

3Biology Department, Faculty of Science-Taif University, Taif-KSA

*Corresponding Author's Email:

Accepted 31 December, 2017



Regulation of insulin gene expression in response to increases in blood glucose levels is essential for maintaining normal glucose homeostasis; however, the exact mechanisms by which glucose stimulates insulin gene transcription are unclear. Olive leaves extracts(OLE) are reported to have beneficial effects on people with normal and impaired glucose tolerance, the metabolic syndrome, type 2 diabetes, and insulin resistance. However, clinical results are controversial and the molecular characterization of OLE effects is limited. This study investigated the effects of OLE, prepared from wild olive leaves, on gene expression of insulin and glucagon in mice tissues. Diabetes in mice was induced by intraperitoneal injections of alloxan. The alterations in gene expressions of pancreatic insulin and hepatic glucagon were evaluated throughout the RT-PCR analysis. The results indicated that diabetic mice showed significantly increased in the expression of pancreatic insulin gene followed by significantly decreased in the expression of hepatic glucagon gene. The administration of OLE at 0.33 g/kg for six days before alloxan treatment; up-regulated pancreatic insulin and modulated hepatic glucagon genes expression. Meanwhile, administration of OLE after induced diabetic mice showed a highly significant increase in gene expression of pancreatic insulin followed by significantly decreased in gene expression of hepatic glucagon. Results suggest that the OLE possess a potent anti-hyperglycemic effect, which may be due to the presence of antioxidants such as polyphenols, these antioxidant activities restraining the oxidative stress which is widely associated with diabetes pathologies and complications.

Keywords: Olive leaves, Insulin, Glucagon, Diabetes, Gene expression.





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