Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
May 2020 Vol. 9(5): pp. 100-109
Copyright © 2020 Global Advanced Research Journals
Full Length Research Paper
Biodegradation of Paraeforce Using Yeast Cells Isolated From Arable Farmland in Obio/Akpor Local Government Area of Rivers State.
Onianwah, F. I¹.; Eze, V. C¹.; Ifeanyi¹, V. O.; Stanley, H.O².
1Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria.
2University of Port Harcourt, Port Harcourt, Rivers State, Nigeria.
*Corresponding Author's Email: onianwah2208@gmail.com
Accepted 17 May, 2020
Abstract
The aim of the research is explore the biodegradation potential of yeast on paraeforce impacted soil and the analysis of the associated enzymes and metabolites. Soil sample was collected from an agrarian soil previously exposed to paraeforce herbicide. Physicochemical analysis was done on the soil sample. Fungal isolation was done using traditional plate culture and molecular techniques. The biodegradation potential of the isolates was determined using titrimetric method. The physicochemical analysis of the treated soil sample recorded a pH of 6.96, temperature (330C), moisture content (71.8%), nitrate (25.6mg/kg), total organic carbon (42mg/kg), biochemical oxygen demand (5.68mg/kg), and conductivity (4.236µscm-1). Yeasts isolated from the soil sample were Pichia kudriavzevii MT366877, Hanseniaspora opuntiae MT366875, Candida. These yeasts were able to degrade the paraeforce. There was a synergy in the degradative activity of the mixed culture. At the end of 90 days, paraeforce degradation was significant in Pichia kudriavzevii MT366877 (27.5mg/kg), Hanseniaspora opuntiae MT366875 (22.4mg/kg) and Pichia cecembensis MT366876 (23.1mg/kg). Natural attenuation recorded a degradation rate of 41.37mg/kg in 90 days. When the sample was optimized using poultry wastes, the degradation rate improved. The mixed culture achieved 100% (50mg/kg) degradation of paraeforce in 56 days. Natural attenuation achieved 100% degradation in 70 days. Pichia kudriavzevii MT366877, Hanseniaspora opuntiae MT366875 and Pichia cecembensis MT366876 recorded 36.7mg/kg, 29.5mg/kg and 26.08mg/kg respectively at the end of 90 days. The growth of fungi was influenced by pH, temperature, nitrate, total organic carbon and the biochemical oxygen demand of the growth medium. The research showed that living cells of the yeasts have great potential for the degradation of paraeforce in an impacted soil and may be used bioremediation of impacted soil.
Keywords: Biodegradation, Bioremediation, Contamination, Paraeforce, Soil And Yeasts.
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