Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
May 2020 Vol. 9(5): pp. 121-127
Copyright © 2020 Global Advanced Research Journals
Full Length Research Paper
Isolation and Characterization of Paraeforce Tolerance and Utilizing Fungi in Soil Samples Collected From an Exposed Arable Farmland
Onianwah, F. I. Eze, V. C., Ifeanyi, V. O
Department of Microbiology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria.
*Corresponding Author's Email: firstname.lastname@example.org
Accepted 23 May, 2020
The aim of this study is to isolate and characterize paraeforce tolerance and utilizing fungi from an arable farmland previously exposed to the herbicide paraeforce. Soil samples were collected in sterile containers and aseptically cultured on potato dextrose agar using a streak plate culture technique. The fungal isolates were screened for tolerance and utilization of paraeforce using 50mg/l concentration. The screened fungal species were further characterized using molecular method. The presumptive identification of fungal isolates based on colonial and cell morphology showed the presence of Saccharomyces, Aspergillus, Pichia, Candida, Rhizopus, Penicillium, Trichoderma and Fasurium species. The screening test done showed that of these isolates, only Saccharomyces, Aspergillus and Candida were able to grow on paraeforce medium. The molecular characterization proved these screened fumgal species to be Pichia krudiavzevii, Pichia cecembensis, Hanseniaspora opuntiae and Aspergillus spinosus. However, a more vigorous growth was observed in the mold (Aspergillus spinosus) than the yeast counterparts. This is due to their filamentous nature and their ability to produce extracellular enzymes.
Keywords: Isolation, Characterization, Paraeforce, Plate Culture and Molecular Technique.
Amakiri MA (1982). Microbial Degradation of soil Applied Herbicides. Nigerian Journal of Microbiology 2:17-21.
Aneja KR (2005). Experiments in Microbiology, Plant pathology and Biotechnology. 4th Edn. New Age International Pvt, Ltd, India.
Baldwin BC, Bray MF, Goeghegan MJ (1977). The Microbial Decomposition of Paraquat, Biochem. Journal, 101:15-17,
Baran N, Mouvet C, Negrel P (2007). Hydrodynamic and geochemical constraints on pesticide concentrations in the groundwater of anagricultural catchment (Brevilles, France). Environmental Pollution, 148 (3): 729-738.
Barnett HL, Hunter B (1972). Illustrated genera of imperfecti fungi, 3rd edn. Burges publishing company, USA.
Belal EB, Zidan NA, Mahmoud HA, Eissa FI (2008). Bioremediation of pesticides– contaminated soils. J. Agric. Res. Kafrelsheikh. Univ. 34: 588 – 608.
Castillo JM, Casas J, Romero E (2011). Isolation of an endosulfandegrading bacterium from a coffee farm soil: persistence and inhibitory effect on its biological functions. Sci. Total Environ. 2011: 412-413
Cheesbrough M (2001). Medical Laboratory Manual for Tropical Countries, Microbiology Educational Book Scheme.2: 389-90
Felsenstein J (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791.
Das N, Chandran P (2011). Microbial degradation of petroleum hydrocarbon contaminants: An overview. Biotechnol. Res. Inter., 2011: 1 13.
Gadd GM (2001). Fungi in bioremediation. Cambridge: Cambridge University Press.
Holt JG, Krieg NR, Sneath PHA, Williams ST, Eds. (1994). Bergey’s manual of determinative bacteriology, 9th ed. Baltimore: Williams and Wilkins.
Kodama T, Ding L, Yoshida M, Yajima M (2001). Biodegradation of an s-triazine herbicide, simazine, Journal of Molecular Catalysis B: Enzymatic, 11(4):1073–1078.
Okpokwasili GC, Nwosu AT (1990). Degradation of Aldrin by Bacteria Isolates. Nigerian Journal of Technological Research. 2, 1-6.
Onianwah FI (unpublished). Biodegradation potential of fungi on paraeforce impacted soil and analysis of associated enzymes and metabolites. Ph.D thesis, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria.
Oxoid manual (2007). sixth edition (reprint). Published by Oxoid limited, Wade Road, Basingstoke Hampshire RG24 OPW. pp105, 172, 182, 192-3, 203-4, 217, 256 and 282.
Rhodes CJ (2012). Mycoremediation (bioremediation with fungi)-growing mushrooms to clean the earth. Chem Speciat Bioavailab. 26:196–198. doi: 10.3184/095422914X14047407349335.
Saparrat MCN, Martínez MJ, Cabello M, Arambarri AM (2002) Screening for ligninolytic enzymes in autochthonous fungal strains from. Argentina isolated from different substrata. Rev Iberoam Micol. 19:181–185.
Saitou N, Nei M (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
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- Ifeanyi on Pubmed
- V. O on Google Scholar
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