Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
May 2020 Vol. 9(5): pp. 121-127
Copyright © 2020 Global Advanced Research Journals
Full Length Research Paper
Isolation and Characterization of Paraeforce Tolerance and Utilizing Fungi in Soil Samples Collected From an Exposed Arable Farmland
Onianwah, F. I. Eze, V. C., Ifeanyi, V. O
Department of Microbiology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria.
*Corresponding Author's Email: onianwah2208@gmail.com
Accepted 23 May, 2020
Abstract
The aim of this study is to isolate and characterize paraeforce tolerance and utilizing fungi from an arable farmland previously exposed to the herbicide paraeforce. Soil samples were collected in sterile containers and aseptically cultured on potato dextrose agar using a streak plate culture technique. The fungal isolates were screened for tolerance and utilization of paraeforce using 50mg/l concentration. The screened fungal species were further characterized using molecular method. The presumptive identification of fungal isolates based on colonial and cell morphology showed the presence of Saccharomyces, Aspergillus, Pichia, Candida, Rhizopus, Penicillium, Trichoderma and Fasurium species. The screening test done showed that of these isolates, only Saccharomyces, Aspergillus and Candida were able to grow on paraeforce medium. The molecular characterization proved these screened fumgal species to be Pichia krudiavzevii, Pichia cecembensis, Hanseniaspora opuntiae and Aspergillus spinosus. However, a more vigorous growth was observed in the mold (Aspergillus spinosus) than the yeast counterparts. This is due to their filamentous nature and their ability to produce extracellular enzymes.
Keywords: Isolation, Characterization, Paraeforce, Plate Culture and Molecular Technique.
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