Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
June 2016 Vol. 5(6): pp. 195-203
Copyright © 2016 Global Advanced Research Journals


Full Length Research Paper

Purification and Characterization of Acid Phosphatase (AcPase) from Cotyledon of Erythrina indica

Emadeldin Hassan E. Konozy


Biotechnology Park, Africa City of Technology, Khartoum, B. O. Box: 13332, Sudan


Accepted 07 June, 2016



Acid phosphatase (AcPase) (EC is a ubiquitous lysosomal enzyme that hydrolyzes phosphate esters at an acid pH and therefore, plays critical role in plant development and growth. AcPase from seeds of Erythrina indica, a leguminous plant, was extracted and purified 61 folds to apparent electrophoretic homogeneity in 5 purification steps which involved; ammonium sulphate fractionation, ConA-seralose 4B, acidification to pH 5 and finally DEAE-cellulose.  The native molecular weight of the enzyme as estimated by calibrated gelfiltration was 89kDa while 38 and 42kDa were obtained for subunits under reduced denatured SDS-PAGE conditions. AcPase had a broad pH optima from 4.5 to 5.5 with a maxima centered at around pH 5. The enzyme, as well, had a wide range of temperature optima ranging from 30 ͦ C to 55 ͦ C. AcPase activity was totally inhibited with Zn++, while 80% increase in activity was noticed in the presence of Cu++. The enzyme kinetics data, km and Vmax values, for hydrolysis of p-nitrophenyl phosphate were 3.3 and 13, respectively. AcPase was present in at least three isoforms, had a glycoprotein nature with mannose as terminal putative sugar.

Keywords: Acid phosphatase, Erythrina indica, leguminousae, purification, characterization.



Related Articles

Original Research Articles
W. Dib, V. Biscola, Y. Choiset, J-M. Chobert, T. Haertlé, A. Chekroun, D. Saidi, O. Kheroua
Characterization of a new isolate of Lactobacillus brevis WD19 from Algerian goat milk with proteolytic activity
Glo. Adv. Res. J. Agric. Sci. December 2014 Vol: 3(12): - [Abstract] [Full Text - PDF] (333 KB)
Tag Alsir Altayeb Beshier Ahmed, Caroline Edward Ayad, Hussein Ahmed Hassan, Elsafi Ahmed Abdalla and Momen Abdou Elkhir
Characterization of Substantia Nigra in Parkinson disease using MR Imaging
Glo. Adv. Res. J. Agric. Sci. January 2015 Vol: 4(1): - [Abstract] [Full Text - PDF] (144 KB)
Kinsiclounon Gilles E, Edorh Patrick A, Guedenon Patient, Senou Masimin, Deguenon Yvette, Akpovi Casimir, Kaki Christophe Loko Frederic, Boko Michel, Gbeassor Messanvi and Creppy Edmond E
Comparative evaluation of lead, cadmium, nitrates, total iron and their derivatives, in drinking water in the lacustrine township of Sô-Ava between 2009 and 2012
Glo. Adv. Res. J. Agric. Sci. September 2013 Vol: 2(7): - [Abstract] [Full Text - PDF] (448 KB)
Ola-Buraimo AO and Oluwajana OA
Sedimentological characterization and palynological age dating of the pre-bima and bima formations in bornu basin, North-Eastern Nigeria
Glo. Adv. Res. J. Agric. Sci. July 2012 Vol: 1(4): - [Abstract] [Full Text - PDF] (1,221 KB)
Dauda A. O., Salami, K.O., Oyeyinka, S.A., Esan, O.T., Arise, A.K and Asije A.R.
An Assessment of the Preservative Potentials of Soursop (Annonamuricata) Seed Oil on Plantain and Orange Juice.
Glo. Adv. Res. J. Agric. Sci. August 2018 Vol: 7(6): - [Abstract] [Full Text - PDF] (307 KB)
Muhammad Riaz and Abdul Chaudhry
Chemical characterisation of shrubs browses from salt affected soils and Pothohar areas in Pakistan for their nutritional and anti-nutritional compounds
Glo. Adv. Res. J. Agric. Sci. November 2016 Vol: 5(11): - [Abstract] [Full Text - PDF] (141 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Emadeldin Hassan E. Konozy on Google Scholar
Emadeldin Hassan E. Konozy on Pubmed


Viewed 1893
Printed 1955
Downloaded 1012
Powered By iPortal Works