Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
June 2016 Vol. 5(6): pp. 195-203
Copyright © 2016 Global Advanced Research Journals

 

Full Length Research Paper

Purification and Characterization of Acid Phosphatase (AcPase) from Cotyledon of Erythrina indica

Emadeldin Hassan E. Konozy

 

Biotechnology Park, Africa City of Technology, Khartoum, B. O. Box: 13332, Sudan

Email: ehkonozy@yahoo.com

Accepted 07 June, 2016

 

Abstract

Acid phosphatase (AcPase) (EC 3.1.3.2) is a ubiquitous lysosomal enzyme that hydrolyzes phosphate esters at an acid pH and therefore, plays critical role in plant development and growth. AcPase from seeds of Erythrina indica, a leguminous plant, was extracted and purified 61 folds to apparent electrophoretic homogeneity in 5 purification steps which involved; ammonium sulphate fractionation, ConA-seralose 4B, acidification to pH 5 and finally DEAE-cellulose.  The native molecular weight of the enzyme as estimated by calibrated gelfiltration was 89kDa while 38 and 42kDa were obtained for subunits under reduced denatured SDS-PAGE conditions. AcPase had a broad pH optima from 4.5 to 5.5 with a maxima centered at around pH 5. The enzyme, as well, had a wide range of temperature optima ranging from 30 ͦ C to 55 ͦ C. AcPase activity was totally inhibited with Zn++, while 80% increase in activity was noticed in the presence of Cu++. The enzyme kinetics data, km and Vmax values, for hydrolysis of p-nitrophenyl phosphate were 3.3 and 13, respectively. AcPase was present in at least three isoforms, had a glycoprotein nature with mannose as terminal putative sugar.

Keywords: Acid phosphatase, Erythrina indica, leguminousae, purification, characterization.

   


 

Related Articles


Original Research Articles
I.I. Fragoso-Castro, M. Cornejo-Mazón, O.A. Ramos-Monroy, M .T. Hernández-Botello, P. Rosales-Martínez, M.S. López-Cortez
Berries Harvested in Mexico and its Use to Prepare a Functional Drink Conserved By Ultrasonication
Glo. Adv. Res. J. Agric. Sci. February 2019 Vol: 8(2): - [Abstract] [Full Text - PDF] (847 KB)
Emadeldin Hassan E. Konozy
Isolation, Purification and Characterization of a Thermostable β-Galactosidase isoform from Erythrina indica Seeds
Glo. Adv. Res. J. Agric. Sci. June 2016 Vol: 5(6): - [Abstract] [Full Text - PDF] (963 KB)
SMJ Hossain, MF Afroz, AKFH Bhuiyan and MA Habib
Milk Production Performance of Red Chittagong Cattle Based on Phenotypic and Genetic Parameters
Glo. Adv. Res. J. Agric. Sci. April 2018 Vol: 7(4): - [Abstract] [Full Text - PDF] (174 KB)
Shimaa E. Ibrahim, Hala F Mohamed, Heba Sh. Shehata, and Rawheya A. Salah El Din
Perineal Plant Extract as a Culture Medium for Production of Plant Growth Regulators by Molecularly Identified Rhizospheric Microorganisms
Glo. Adv. Res. J. Agric. Sci. February 2020 Vol: 9(2): - [Abstract] [Full Text - PDF] (1,286 KB)
Adel S Al-Zubairi, Mohamed A Al-Mamary and Eftekhar Al-Ghasani
The Antibacterial, Antifungal, and Antioxidant Activities of Essential Oil from Different Aromatic Plants
Glo. Adv. Res. J. Agric. Sci. September 2017 Vol: 6(9): - [Abstract] [Full Text - PDF] (168 KB)
Amal Elsayed Edriss, Zuhair Alfadil Alabjar and Abdalla Abdelrahim Satti
Phytochemical screening of important secondary metabolites in some extracts of two Sudanese plants
Glo. Adv. Res. J. Agric. Sci. November 2012 Vol: 1(8): - [Abstract] [Full Text - PDF] (145 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Emadeldin Hassan E. Konozy on Google Scholar
Emadeldin Hassan E. Konozy on Pubmed

Statistics

Viewed 2016
Printed 1969
Downloaded 1049
Powered By iPortal Works