Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
July 2015 Vol. 4(7): pp. 280-301
Copyright © 2015 Global Advanced Research Journals


Full Length Research Paper

Efficient in vitro production of non-chimeric tetraploid in kale (Brassica oleracea L. var. acephala) and its evaluation as a functional crop

Hiroyuki Niimi1, Masami Watanabe1, Yoshiaki Serizawa2, Takato Koba1, Ikuo Nakamura1 and Masahiro Mii1*


1Graduate School of Horticulture, Chiba University,648 Matsudo, Matsudo, Chiba 271-8510, Japan

2Nagano Vegetable and Ornamental Crops Experiment Station,1066-1 Tokoo, Souga, Shiojiri City, Nagano 399-6461, Japan

*Corresponding Author’s E-mail:;

Tel & Fax: +81-4-7137-8177.

Accepted 05 June, 2015



Kale (B. oleraceavar. acephala) is a kind of vegetables with high content of glucosinolates (GSL) such as glucoraphanin (GRA), which are known to be functional ingredients. In this study, efficient method of tetraploid production in kale was established by treating seeds, in vitrogerminated5 day-old seedlings and in vitro-propagated plantlets having 3 or 4 leaves, with either colchicine or amiprophos-methyl (APM), followed by in vitro culture. The highest frequency of tetraploid plant production (24.0%) was obtained when the seeds were treated with APM at 9 mg l-1 for 24 hours. Moreover, non-chimeric tetraploid can be efficiently selected by measuring fresh weight 7 days after the treatment. These tetraploid plants had gbigger sizes of leaf, flower and pollen and more number of leaves than diploid, but showed comparable content of GRA to diploid.    

Keywords: Amiprophos-methyl, Brassica oleracea var. acephala, colchicine, glucosinolate, in vitro culture, kale, tetraploid.


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