Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
July 2019 Vol. 8(6): pp. 185-193
Copyright © 2019 Global Advanced Research Journals


Full Length Research Paper

Characterization of an anaerobic bacterial consortium isolated from chicken manure capable to degrade organic arsenical compound into inorganic arsenic and methane

Javiera Ravanal1, Ruben Moraga2, Carla G. Leon1, Italo A. Fernandez1, Rodrigo Riquelme1, Victor Hernández3, Jorge Yañez4., Carlos T. Smith1, Maria A. Mondaca1, Victor L. Campos1

1Environmental Microbiology Laboratory, Department of Microbiology, Faculty of Biological Sciences, University of Concepción, Concepción, Chile.

2Microbiology Laboratory, Faculty of Renewable Natural Resources, Arturo Prat University, Iquique, Chile

3Natural Products Chemistry Laboratory, Department of Botany, University of Concepción, Chile.

4Department of Analytical and Inorganic Chemistry, Faculty of Chemical Sciences, University of Concepción, Chile.

*Corresponding Author's Email:

Accepted 19 May, 2019



Roxarsone (ROX) (3-nitro-4-hydroxybenzene arsenic acid), an arsenic (As) containing compound is widely used as a food additive in the production of broiler chickens to control coccidial intestinal parasites and to favour rapid growth. Broiler chickens receiving ROX in their diet (between 23 and 45 g ton-1 food) excrete it untransformed in manure. This manure is commonly used as fertilizer, polluting farming fields. However, several soil bacteria can degrade ROX, releasing inorganic As. The results of this work demonstrated that a bacterial consortium, isolated from chicken manure and cultured under anaerobic conditions, was mainly composed by bacilli. DGGE analysis of the 16s rDNA sequences demonstrated that Firmicutes, which has been reported as main components in soils, sediments and animal faeces under anaerobic conditions, was the predominant tax a present in the studied consortium. The growth kinetics of the consortium was higher in the presence of ROX than in its absence, suggesting that ROX could be used as carbon source by the consortium. ROX was degraded by the consortium producing inorganic As, mainly arsenite (As(III)). Concomitantly with ROX biotransformation, the consortium produced As free methane. These results provide the first evidence that an anaerobic bacteria consortium isolated from chicken manure can rapidly biotras form ROX to inorganic arsenic and produce arsenic free biogas.

Keywords: biotransformation, roxarsone, chicken manure, bacterial-community, arsenic, methane.







Campos VL, León C, Mondaca MA, Yañez J, Zaror C (2011). Arsenic Mobilization by Epilithic Bacterial Communities Associated with Volcanic Rocks from Camarones River, Atacama Desert, Northern Chile. Arch. Environ. Contam. Toxicol. 61:185–192.

Chapman HD, Johnson ZB (2002). Use of antibiotics and roxarsone in broiler chickens in the USA: analysis for the years 1995 to 2000. Poult. Sci. 81:356–364.

Cortinas                    I,    Field    J,    Kopplin    M,     Garbarino    J, Gandolfi       A,     Sierra-Alvarez      R   (2006).  Anaerobic

biotransformation  of  roxarsone  and  related  N-substituted  phenylarsonic  acids.  Environ.  Sci.  Technol. 40:2951–2957.

D’Angelo EM, Zeigler G, Beck EG, Grove J, Sikora F (2012). Arsenic species in broiler (Gallus gallus domesticus) litter, soils, maize (Zea mays L.), and groundwater from litter-amended fields. Sci. Total Environ. 438:286–292.

FDA. 2015. FDA Announces Pending Withdrawal of Approval of Nitarsone.

Garbarino JR, Bednar AJ, Rutherford DW, Beyer RS, Wershaw RL (2003). Environmental fate of roxarsone in poultry litter. I. Degradation of roxarsone during composting. Environ. Sci. Technol. 37:1509–1514.

Guzmán-Fierro  VG,  Moraga  R,  León  CG,  Campos  VL,  Smith  C,  Mondaca  MA  (2015).  Isolation  and characterization of an aerobic bacterial consortium able to degrade roxarsone. Int. J. Environ. Sci. Technol. 12(4):1353–1362.

Han L, Yang G, Zhou X, Yang D, Hu P, Lu Q, Zhou S (2013). Bacillus thermocopriae sp. nov., isolated from a compost. Int. J. Syst. Evol. Microbiol. 63:3024–3029.

Hancock T, Denver J, Riedel G, Miller C (2002) Source transport and fate of arsenic in the Pocomoke River

Basin a poultry dominated Chesapeake Bay Watershed. In: Proceedings of Arsenic in  the Environment Workshop February 21–22 2001 US Geological Survey Denver Colorado.

Huang K, Peng H, Gao F, Liu Q, Lu X, Shen Q, Le XC, Zhao FJ (2019). Biotransformation of arsenic-containing roxarsone by an aerobic soil bacterium Enterobacter sp. CZ-1. Environ. Pollut. 247: 482-487.

Jiang Z, Li P, Wang YH, Li B, Wang YX (2013) Effects of roxarsone on the functional diversity of soil microbial community. Int. Biodeterior. Biodegrad. 76:32–35.

Leon C, Campos V, Urrutia R, Mondaca M (2012). Metabolic and molecular characterization of bacterial community  associated  to  Patagonian  Chilean  oligotrophic-lakes  of  quaternary  glacial  origin.  World  J. Microbiol. Biotechnol. 28:1511–1521

Liang T, Ke Z, Chen Q, Liu L, Chen G (2014). Degradation of Roxarsone in a Silt Loam Soil and Its Toxicity Assessment. Chemosphere. 112:128-133.

Liu H, Wang G, Ge J, Liu L, Chen G (2014). Fate of roxarsone during biological nitrogen removal process in wastewater treatment systems. Chem. Eng. J. 255:500–505.

Mafla S, Moraga S, León CG, Guzmán-Fierro V, Yañez J, Smith CT, Mondaca MA, Campos VL (2015).

Biodegradation of Roxarsone by a Bacterial Community of Underground Water and Its Toxic Impact. World J. Microbiol. Biotechnol. 31:1267-1277.

Manzano  P,  Miranda  m,  Abreu  j,  Silva  M,  Hernández  V,  Peralta  E  (2013).  Gas  Chromatography-Mass

Spectrometry  Study  from  the  Leaves  Fractions  Obtained  of  Vernonanthura  patens  (Kunth)  H.  Rob. International Journal of Organic Chemistry 3:105-109.

Mangalgiri KP, Adak A, Blaney L (2015). Organoarsenicals in poultry litter: Detection, fate, and toxicity. Environ. Int. 75:68–80.

Nachman KE, Graham JP, Price LB, Silbergeld EK (2005). Arsenic: a roadblock to potential animal waste management solutions. Environ. Health Perspect. 113:1123.

Nigra AE, Nachman KE, Love DC, Grau-Perez M, Navas-Acien A (2017). Poultry consumption and arsenic exposure in the U.S. population. Environ. Health Perspect. 125:370–377;

Sierra-Alvarez R, Cortinas I, Yenal U, Field J (2004). Methanogenic Inhibition by Arsenic Compounds. Appl. Environ. Microb. 40:5688-5691.

Sierra-Alvarez  R,  Cortinas  I,  Field  J  (2010).  Methanogenic  inhibition  by  roxarsone  (4-hydroxy-3-nitrophenylarsonic acid) and related aromatic arsenic compounds. J. Hazard Mater. 175:352-358.

Stolz JF, Perera E, Kilonzo B, Kail B, Crable B, Fisher E, Ranganathan M, Wormer L, Basu P (2007). Biotransformation of 3-nitro-4-hydroxybenzene arsonic acid (roxarsone) and release of inorganic arsenic by Clostridium species. Environ. Sci. Technol. 41:818–823.

Turpeinen R, Kallio MP, Kairesalo T (2002). Role of microbes in controlling the speciation of arsenic and production of arsines in contaminated soils. Sci. Total Environ. 285:133-145.

Wershaw RL, Garbarino JR, Burkhardt MR (1999). Roxarsone in natural water systems. In: Effects of animal feeding operations on water resources and the environment. US Geological Survey. Open-File Report 00-204, p 95.

Wiegel J (2009). Family I. Clostridiaceae Pribram 1933, 90AL. In: Whitman WB (eds) Bergey’s manual of systematic bacteriology, Volume Three: The Firmicutes, 2nd edn. Springer, New York, 737.

Yañez J, Mansilla H, Santander P, Fierro V, Cornejo L, Barnes M, Amarasiriwardena D (2015) Urinary arsenic speciation profile in ethnic group of the Atacama desert (Chile) exposed to variable arsenic levels in drinking water. J. Environ. Sci. Heal. A 50:1–8.

Yao L, Huang L, He Z, Zhou C, Li G (2013). Occurrence of arsenic impurities in organoarsenics and animal feeds. J. Agric. Food Chem. 61:320–324.

Yin Y, Wan J, Li S, Li H, Dagot C, Wang Y (2018). Transformation of roxarsone in the anoxic–oxic process when treatingthe livestock wastewater. Sci. Total Environ. 616:1224–1234

Zhang FF, Wang W, Yuan SJ, Hu ZH (2014) Biodegradation and speciation of roxarsone in an anaerobic granularsludge system and its impacts. J. Hazard Mater. 279:562–568.

Zwietering MH, Jongenburger I, Rombouts FM, Van't Riet K (1990) Modeling of the bacterial growth curve. Appl. Environ. Microbiol. 56:1875-1881.












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