Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
September 2013 Vol. 2(9): pp. 246-251
Copyright © 2015 Global Advanced Research Journals
Full Length Research Paper
Methods for the screening of sorghum germplasm against sorghum head and loose smuts in Nigeria
* Kutama1, A.S., 2Umar, S., 2Binta, U.B. and 2Tijjani, A.
1Department of Biological Sciences, Faculty of Science, Federal University, Dutse, Jigawa state, Nigeria
2Department of Biological Sciences, College of Arts, Sciences and Remedial Studies, Kano
*Corresponding Author’s Email: firstname.lastname@example.org;
Accepted 30 September, 2013
Five artificial inoculation techniques which includes, stem injection, seed, soil, PDA-seedling, and cotton wool-seedling inoculation techniques were evaluated to determine the incidence and severity of sorghum head and loose smuts in two sorghum genotypes: SSV2006002 and SSV2008089 under field conditions for two growing seasons (2009 and 2010). The trials were carried out at the teaching and research farm of Bayero University, Kano, Nigeria, and laid out in a split plot design in which the sorghum genotypes occupied the main plots while inoculation techniques were the sub plots. Disease incidence varied significantly (P<0.05) between the different inoculation techniques and sorghum genotypes. For head smut, stem injection >PDA>, soil > cotton was the order of decreasing % disease incidence. No head smut infection occurred following seed inoculation in both the sorghum genotypes, of which SSV2008089 was more susceptible than SSV2006002. For loose smut, stem injection> seed > soil > PDA> cotton was the order of decreasing % disease incidence. So, all the techniques produced some diseased plants, with significant (P<0.05) difference between the inoculation techniques. Similarly, SSV2008089 had the higher disease incidence than SSV2006002. The severity also followed the same trend. Injection method produced most severely infected sorghum genotypes for both head and loose smuts with significant difference (P<0.05). Therefore, all the five artificial inoculation methods could be used to screen sorghum germplasm against head and loose smuts.
Keywords: inoculation methods, sorghum genotypes, head and loose smuts, disease incidence and severity.
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- Binta UB on Google Scholar
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- Tijjani A on Google Scholar
- Tijjani A on Pubmed