Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
September 2015 Vol. 4(9): pp. 517-532
Copyright © 2015 Global Advanced Research Journals

 

Full Length Research Paper

Identification and Improving Antibiotic Production by Some Bacteria Isolated From Egyptian Soil

Mohamed T. Shaaban1, M. Attia2, Eman I. Mowafy2*, Azza Sh. Turky2 and Nemat M. Awad2

 

1Botany Dept. Faculty of Sci. Minufiya University 

2Agricultural Microbiology Dept., National Research Centre, 33 El-Bohouth St., (former El- Tahrir St.,) Dokki,Giza, Egypt. Postal Code: 12622.

*Corresponding Author’s Email: eman_mowafy2007@hotmail.com;

Tel: +201002940449, 0226335595; Fax: 0202 6203731.

Accepted 16 September, 2015

 

 

Abstract

The three isolates (SOF12, AMF11 and SMF4) showed highly capability for antimicrobial activity against all the tested pathogenic bacteria with high potential inhibitory activity against yeast and two filamentous plant pathogenic fungi were prescreening studies for bioassay against crude extracts and assayed for their minimal inhibitory concentrations (MIC). The result showed that, crude extract tested positive in bioassay against the all tested pathogenic bacteria while different between them. MIC values ranging from 0.0625 to 5 mg/ml. Based on it’s morphological and microscopy characteristics as well as 16S rRNA sequence analysis, the three isolates designated as SOF12, AMF11 and SMF4 were identified as identified Bacillus megaterium TDB-13,Bacillus licheniformis DV7 and Bacillus subtilis 003141, respectively. To study the effect of some growth factors on the production of antagonistic bacteria metabolites against selected human, plant pathogenic bacteria and plant pathogenic fungi and yeast. The results showed that the antimicrobial activity of the metabolites increased generally from the first to the 9 day of incubation, the optimum temperature for maximum activity by B. megaterium and B. licheniformis was observed at 30°C while B. Subtilis at 25°C, the optimum pH for B. megaterium and B. licheniformis was 7 whereas B. subtilis at pH 8, the best carbon sources for B. megaterium was starch and asparagine, while B. licheniformis, starch and glycerol, but B. subtilis glycerol. The asparagine was observed as the best nitrogen source for all the strains.

Keywords:  Antimicrobial agent, Bacterial isolates, growth factors, 16S rRNA gene.

 


 

Related Articles


Original Research Articles
CI Mboto and P Udoh
Susceptibility of Proteus mirabilis, Staphylococcus aureus and Candida albicans to Extracts of Cola acuminata.
Glo. Adv. Res. J. Agric. Sci. June 2014 Vol: 3(5): - [Abstract] [Full Text - PDF] (134 KB)
W. Braide, S.U.Oranusi, C.K. Akaluka , R.N.Nwaoguikpe, C.I. Akobundu and N.I. Peter-Ikechukwu
Antibacterial efficacy of crude and diluted honey on four wound isolates
Glo. Adv. Res. J. Agric. Sci. February 2012 Vol: 1(1): - [Abstract] [Full Text - PDF] (65 KB)
Salinas-Castro A, Nava-Díaz C, Luna- Rodríguez M, San Martín-Romero E, Rivera-Fernández A, Trigos A
Antagonistic bacteria affecting the Golden cyst potato nematode (Globoderarostochiensis Woll.) in the region of Perote, Veracruz, México
Glo. Adv. Res. J. Agric. Sci. February 2016 Vol: 5(2): - [Abstract] [Full Text - PDF] (180 KB)
Nagwa Thabet Elsharawy
The Inhibition Effect of Thyme and Coriander Essential Oils on of Campylobacter Jejuni and Some of Their Virulence Genes on Chicken Burger and Chicken Shawerma Products
Glo. Adv. Res. J. Agric. Sci. August 2018 Vol: 7(6): - [Abstract] [Full Text - PDF] (1,879 KB)
Asmaa A. Alharbi
Molecular Characterization Using 16S rRNA Gene of Isolated Bacterial Genera Associated With Mango Cultivation in Jazan province, South West KSA
Glo. Adv. Res. J. Agric. Sci. September 2017 Vol: 6(9): - [Abstract] [Full Text - PDF] (1,669 KB)
I Wayan Suardana, Annisa Putri Cahyani and Komang Januartha Putra Pinatih
Probiotic Potency and Molecular Identification of Lactic Acid Bacteria Isolated from Bali Cattle’s Colon, Indonesia
Glo. Adv. Res. J. Agric. Sci. May 2016 Vol: 5(5): - [Abstract] [Full Text - PDF] (124 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Mohamed T. Shaaban on Google Scholar
Mohamed T. Shaaban on Pubmed
M. Attia on Google Scholar
M. Attia on Pubmed
Eman I. Mowafy on Google Scholar
Eman I. Mowafy on Pubmed
Azza Sh. Turky on Google Scholar
Azza Sh. Turky on Pubmed
Nemat M. Awad on Google Scholar
Nemat M. Awad on Pubmed

Statistics

Viewed 1800
Printed 382
Downloaded 1822
Powered By iPortal Works