Global Advanced Research Journal of Agricultural Science (GARJAS) ISSN: 2315-5094
September 2017 Vol. 6(9): pp. 285-292
Copyright © 2017 Global Advanced Research Journals

Full Length Research Paper

Molecular Characterization Using 16S rRNA Gene of Isolated Bacterial Genera Associated With Mango Cultivation in Jazan province, South West KSA

Asmaa A. Alharbi

Department of Biology, Faculty of Science, Jazan University, Kingdom of Saudi Arabia

Email: aalharbi@jazanu.edu.sa

Accepted 24 September, 2017

Abstract

Three hundred and fifty mango soil and root samples were collected from Jazan province south west KSA in order to examine the presence of different bacterial genera distributed in this area. A total of 13 bacterial isolates were identified by 16S rRNA sequencing and using BLAST. The neighbor-joining method was used to produce a Molecular Phylogenetic analysis and a phylogenetic tree was constructed. As a result of this study the following bacteria Bacillus sp. (1), B. firmus (1), B. licheniformis (1), B. subterraneus (2), B. subtilis (1), B. vireti (1), Fictibacillus barbaricus (1), Lysobacter enzymogenes (1), Paenibacillus sp. (1), Paracoccus sp. (1), Streptomyces sp. (1), S. griseorubens(1) were found. Isolated bacteria are significantly important because they regulate soil ecosystem. To the best of our knowledge this is the first report of identified bacterial isolates associated with mango soil samples according to 16S rRNA gene in Jazan province, KSA.

Keywords:  Bacteria, 16S rRNA, Survey, Mang, soil, Abu Arish, Sabia.