Global Advanced Research Journal of Biotechnology (GARJB)

January 2013 Vol. 2(1), pp 008-012

Copyright © 2013 Global Advanced Research Journals

 

Full Length Research Paper

Antibody responses to a recombinant Onchocerca volvulus antigen (Ov1.9) by onchocerciasis patients before and two months after Ivermectin treatment

Osue HO1, Edeghere H2, Galadima M3, Odama LD4 and Engelbrecht F5

1Nigerian Institute for Trypanosomiasis Research (N. I. T. R.), P.M.B. 2077, Kaduna, Nigeria

2National Onchocerciasis Control Programme (NOCP), Race Course Road, Kaduna.

3Formerly of Microbiology Department, Faculty of Science, Ahmadu Bello University (A B. U.), Zaria, Kaduna State, Nigeria

4National Pharmaceutical Research and Development, Idun, Abuja, Nigeria

5Hygeuine Institue, Abt. Parasitologie Im Neunheimer Feld 32469120 Heiderbeg, Germany.

Corresponding author E-mail: Osueho@yahoo.com

Accepted 23 May, 2012

Abstract

Indirect sandwich enzyme-linked immunosorbent assay (ELISA) was used to measure serum IgG antibody responses to Onchocerca volvulus designated (Ov1.9) recombinant antigen in onchocerciasis patients (n=114) prior to initial ivermectin treatment. Paired sera (n=32) obtained 2months after treatment were also analyzed for changes in antibody levels in a sub-population at pre- and post-treatment. The difference between mean optical density (OD) values of IgG antibody level in proven cases (n=95) and the assumed endemic-normal (EN, n=19) of 0.58±0.23 versus 0.37±0.21, respectively was statistically significant (P<0.05) by the t-test of unpaired data. The non-endemic (NEN, n=7) control had mean OD value of 0.12±0.02. There was no significant difference between the sexes or among age groups and the infection subgroups (with or without presence of nodule and/or skin microfilariae mf). At a cut-off point of mean plus 2 standard deviations of OD value for the NEN control, the sensitivity of 87% in proven cases of onchocerciasis (n=95) and 89% in those with skin mf positive (n=62) proved that Ov1.9 may be suitable not as a single screening test reagent, but when in cocktail with one or more antigens. The higher mean OD value in those with chronic skin disease than those without clinical signs of onchocerciasis (n=39); with papular onchodermatitis (n=20) and optic nerve disease (n=12) subgroups was statistically significant by t-test of unpaired data (P<0.05). The heightened IgG antibody response to Ov1.9 with chronic skin disease is clearly indicative of the possible role parasite-specific antibodies may play in the immunopathology of the disease. There was no significant increase in mean antibody level two month post-ivermectin treatment; hence the antigen will not be useful for drug screening.   

Keywords: Serology, sensitivity, Screening, DiagnosisAntigens and Antibodies.

 

 

Related Articles


Original Research Articles
Silke Anna Theresa Weber, Carlos Alberto dos Santos Filho, Arthur Oscar Schelp, Luiz Antonio Lima Resende, João Paulo Papa, and Christian Hook
Classification of handwriting patterns in patients with Parkinson´s disease, using a biometric sensor
Glo. Adv. Res. J. Biotechnol. November 2014 Vol: 3(11): - [Abstract] [Full Text - PDF] (216 KB)
Awoniyi AO, Komolafe OI, Bifarin O and Olaniran Olarinde
Bacterial vaginosis among pregnant women attending a primary health care center in Ile-Ife, Nigeria
Glo. Adv. Res. J. Biotechnol. January 2015 Vol: 4(1): - [Abstract] [Full Text - PDF] (106 KB)
Zia Ur Rahman Awan, Abdul Haleem Shah, Hafiz Munib Ur Rahman, and Sanaullah Khan
Hepatitis C Virus infection in IDPs of war against terrorism in South Waziristan Agency, Pakistan
Glo. Adv. Res. J. Biotechnol. September 2012 Vol: 1(2): - [Abstract] [Full Text - PDF] (714 KB)
Irshad M, Asif Iqbal, Ansari MA and Raghavendra L
Relation of insulin resistance (IR) with viral etiology and blood level of cytokines in patients with liver diseases
Glo. Adv. Res. J. Biotechnol. March 2013 Vol: 2(3): - [Abstract] [Full Text - PDF] (280 KB)
Waseem S Adam, Jamila K Adam and Neil Hendricks
Diagnostic value of signal-averaged electrocardiogram in arrythmogenic right ventricular cardiomyopathy patients
Glo. Adv. Res. J. Biotechnol. July 2013 Vol: 2(7): - [Abstract] [Full Text - PDF] (181 KB)
Baba Usman Ahmadu, Rabasa Adamu Ibrahim, Bukbuk David, Musa Alhaji Bukar, Bashir Faruk, Chama Rejoice, Abdulrahim Halima and Fate Bala Zira
Does placental malaria infection impairs the passage of measles antibodies from mothers to their newborn infants?
Glo. Adv. Res. J. Biotechnol. January 2013 Vol: 2(1): - [Abstract] [Full Text - PDF] (163 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Osue HO on Google Scholar
Osue HO on Pubmed
Edeghere H on Google Scholar
Edeghere H on Pubmed
Galadima M on Google Scholar
Galadima M on Pubmed
Odama LD on Google Scholar
Odama LD on Pubmed
Engelbrecht F on Google Scholar
Engelbrecht F on Pubmed

Statistics

Viewed 1114
Printed 334
Downloaded 988
Powered By iPortal Works