Global Advanced Research Journal of Biotechnology (GARJB)

October 2012 Vol. 1(3), pp 040-047

Copyright © 2012 Global Advanced Research Journals

Full Length Research Paper

Organogenesis and Somatic Embryogenesis in various Cultivars of Codiaeum Variegatum (L.)

Sana S, Shiju Mathew* and Krishnapriya RS

Department of Biotechnology, Marthoma College of Science and Technology, Ayur (India)

*Corresponding author E-mail: shijumathewbiotechbiotech@gmail.com

Accepted 10 September, 2012

Abstract

Croton (Codiaeum variegatum) with its amazing colors and leathery leaves is regarded as a beautiful foliage plant. The plant is native of South East Asia. Generally crotons are multiplied vegetatively by means of cuttings and air layering. This process is slow in response and need to maintain large number of mother plants stock. Micropropagation is an alternative mean of propagation that can meet its high demand in relatively shorter time. An improved and enhanced method was established for the In vitro propagation of croton. A series of experiments were conducted for the optimization of shooting and rooting media. enhanced shoots and buds proliferation formation can be achieved by using the MS media with 2mg/L of KIN and BA. The higher concentration of these hormones (5mg/l each) resulted in shoot formation. The In vitro roots were successfully induced by 5.0 mg/L of 2, 4-D. The rooted plants were then effectively acclimatized with the potting mix of 80% sand and 20% farm yard manure, as a potting mix, was best suited for the acclimatization of plantlets.

Keywords:  Tissue Culture, Somatic Embryogenesis, MS Media, Hormones, Acclimatization.