Global Advanced Research Journal of Educational Research and Reviews (GARJERR) ISSN: 2315-5132

February 2016, 5(2): pp. 032-037

Copyright © 2016 Global Advanced Research Journals

Full Length Research Paper

Potential Effect of Camphor Oil as a Local Cercaricidal Agent

Nevine Guirguis Nessim, Soheir Mahmoud, Maisa Metwally, Hoda Sabri and Rabab Zalat

Department of Parasitology, Theodor Bilharz Research Institute, Imbaba, Guiza, Egypt.

Corresponding Author Email: nevinenessim@hotmail.com

Accepted 01 February, 2016

Abstract

Objectives: - The aim of this study is to clarify the deleterious effects of topical Camphor oil administration alone, in experimental Schistosoma mansoni infection .It is a trial to elucidate the stigmata left upon using this compound upon worm and tissue egg loads, together with oogram pattern in experimental murine schistosomiasis mansoni.

Abstract: - In this study, a group of 30 Swiss Albino mice was used. This group was further subdivided into three small subgroups: - Subgroup I: - constituted control infected untreated mice. Infection was done by dermal inoculation of 80 Schistosoma mansoni cercariae per mouse through a ring, after shaving the abdominal skin. Subgroup II: - abdominally shaved mice wiped with 100 µl per mouse Camphor oil, then infected 10 minutes later with 80 Schistosoma mansoni cercariae.

Subgroup III: - abdominally shaved mice wiped with 100 µl per mouse Camphor oil, then infected immediately after with 80 Schistosoma mansoni cercariae.

All animals were sacrificed 6 weeks post infection Mice given Camphor oil, revealed a complete reduction (100 %) in the total worm recovery, when compared to the respective untreated controls (23±2.56). This value was the same when Camphor oil was spread 10 minutes before infection, and immediately after infection with 80 Schistosoma mansoni cercariae. Again, the former group revealed complete disappearance of tissue egg load (both hepatic and intestinal, and vanishing of eggs in the oogram compared to the latter group. as well as healthy hepatic parenchyma.

Keywords: Camphor oil, local cercaricidal, experimental schistosomiasis mansoni, mature worms, tissue egg load oogram and. granuloma measurement.