Gobal Advanced Research Journal of Environmental Science and Toxicology (GARJEST) ISSN: 2315-5140
March 2013 Vol. 2(3), pp. 107-118
Copyright © 2013 Global Advanced Research Journals


Full Length Research Paper

Genotoxicity induced by 4-Nonylphenol in adult and embryos of Clarias gariepinus

Alaa El-Din H. Sayed1, Usama M. Mahmoud1 and Imam A. Mekkawy2

1Zoology department, Faculty of Science, Assiut University, 71516Assiut, Egypt.
2Biology department, Faculty of Science, Taif University, Taif, Saudi Arabia.

*Corresponding Author E-mail: alaa_h254@yahoo.com; Tel: +020882412381; Fax: +020882342708

Accepted  08 December, 2012


The presence of the genetic toxic chemicals in the aquatic environment increased. This study aimed using the random amplified polymorphic DNA (RAPD-PCR) assay to investigate the genotoxic effects of 4-nonylphenol in adults and embryos of African catfish Clarias gariepinus. After exposure of adults and embryos to sublethel concentrations of 4-nonylphenol (0, 0.05, 0.08, and 0.1 mg/l) for 15 days, DNA was extracted for RAPD assay. The RAPD pattern from catfish exposed to 4-nonylphenol displayed some changes in polymorphism band patterns including disappearance and appearance of bands. Cluster method was used to indicate the distinct distance between the band patterns of exposed fish and the control. In conclusion, the RAPD-PCR is method can be used as an investigation tools for the evaluation of the genetic damage of the catfish induced by the exposure to 4-nonylphenol.

Keywords: 4-nonylphenol, genotoxicity, embryos, Clarias, gariepinus, DNA.


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