Gobal Advanced Research Journal of Environmental Science and Toxicology (GARJEST) ISSN: 2315-5140
March 2013 Vol. 2(3), pp. 107-118
Copyright © 2013 Global Advanced Research Journals


Full Length Research Paper

Genotoxicity induced by 4-Nonylphenol in adult and embryos of Clarias gariepinus

Alaa El-Din H. Sayed1, Usama M. Mahmoud1 and Imam A. Mekkawy2

1Zoology department, Faculty of Science, Assiut University, 71516Assiut, Egypt.
2Biology department, Faculty of Science, Taif University, Taif, Saudi Arabia.

*Corresponding Author E-mail: alaa_h254@yahoo.com; Tel: +020882412381; Fax: +020882342708

Accepted  08 December, 2012


The presence of the genetic toxic chemicals in the aquatic environment increased. This study aimed using the random amplified polymorphic DNA (RAPD-PCR) assay to investigate the genotoxic effects of 4-nonylphenol in adults and embryos of African catfish Clarias gariepinus. After exposure of adults and embryos to sublethel concentrations of 4-nonylphenol (0, 0.05, 0.08, and 0.1 mg/l) for 15 days, DNA was extracted for RAPD assay. The RAPD pattern from catfish exposed to 4-nonylphenol displayed some changes in polymorphism band patterns including disappearance and appearance of bands. Cluster method was used to indicate the distinct distance between the band patterns of exposed fish and the control. In conclusion, the RAPD-PCR is method can be used as an investigation tools for the evaluation of the genetic damage of the catfish induced by the exposure to 4-nonylphenol.

Keywords: 4-nonylphenol, genotoxicity, embryos, Clarias, gariepinus, DNA.


Related Articles

Original Research Articles
Imaobong Ekpo E, Mandu Essien Ibok A and Bello Olusoji.
Temporal and seasonal abundance of fish species in Ikot Ebom a tropical rainforest river Nigeria
Glo. Adv. Res. J. Environ. Sci. Toxicol. May 2014 Vol: 3(3): - [Abstract] [Full Text - PDF] (829 KB)
Alaa El-Din H Sayed, Sara S Abdel Hakeem, Usama M Mahmoud and Imam A Mekkawy
4-Nonylphenol induced morphological and histopathological malformations in Bufo regularis tadpoles
Glo. Adv. Res. J. Environ. Sci. Toxicol. September 2012 Vol: 1(6): - [Abstract] [Full Text - PDF] (1,498 KB)
Badr Eldin AE Saeed1, Marmar A. El Siddig2*, Aisha Ohag Osman3 and Adil A. El Hussein2
Development of Regeneration and Transformation Protocol for Local Sudan’s wheat (Triticum aestivum L) Cultivars
Glo. Adv. Res. J. Environ. Sci. Toxicol. August 2015 Vol: 4(1): - [Abstract] [Full Text - PDF] (818 KB)
Musa, H., Mahmud, U., Safiyanu, I., Bashir, S. I. and Kutama, A.S.
Some Ecological Aspects of Bagrusbayad and Clariasgariepinus in Thomas Lake, Kano State, Nigeria
Glo. Adv. Res. J. Environ. Sci. Toxicol. May 2016 Vol: 5(5): - [Abstract] [Full Text - PDF] (600 KB)
Rehab M. Rizk; Magda I. Soliman and Eman M. EL-Zayat
Cytotoxic and Genotoxic Effects of some Narcotic plant extracts using Higher Plant Bioassay
Glo. Adv. Res. J. Environ. Sci. Toxicol. November 2015 Vol: 4(11): - [Abstract] [Full Text - PDF] (1,402 KB)
Avendaño-Arrazate Carlos Hugo, Martínez-Hernández Gregorio, Moreno-Pérez Esaú del C, Sandoval-Esquivez Alfredo, Campos-Rojas Eduardo, Aguirre-Medina Juan F, Cadena-Iñíguez Jorge, Ariza-Flores Rafael.
Morphological Characterization of Mango (Mangiferaindica L.) in Mexico
Glo. Adv. Res. J. Environ. Sci. Toxicol. September 2017 Vol: 6(9): - [Abstract] [Full Text - PDF] (718 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Alaa El-Din H Sayed on Google Scholar
Alaa El-Din H Sayed on Pubmed
Usama M Mahmoud on Google Scholar
Usama M Mahmoud on Pubmed
Imam A Mekkawy on Google Scholar
Imam A Mekkawy on Pubmed


Viewed 1664
Printed 397
Downloaded 1438
Powered By iPortal Works