Gobal Advanced Research Journal of Environmental Science and Toxicology (GARJEST) ISSN: 2315-5140
May 2014 Vol. 3(1), pp. 006-011
Copyright © 2014 Global Advanced Research Journals

 

Full Length Research Paper

Hydrocarbon degradation potentials of indigeneous fungal isolates from a petroleum hydrocarbon contaminated soil in Sakpenwa community, Niger Delta

Iheanacho, C.C.1, Okerentugba, P.O.1, Orji, F.A.2* and Ataikiru, T.L.3

1Department of Microbiology, University of Port Harcourt, P.M.B 5323, Port Harcourt, Nigeria.
2Department of Biotechnology, Federal Institute of Industrial Research, Oshodi, Lagos state, Nigeria.
3Environmental Sciences Department, Federal University of Petroleum Resources, Efurun, Delta State, Nigeria

*Corresponding Author E-mails: phillipokerentugba@yahoo.com; Tel: +2348033087332

Accepted 11 May, 2014

Abstract

In this study, hydrocarbon degradation potentials of indigenous fungal species were investigated employing colonial morphologies and molecular techniques. Soil samples were collected from Saakpenwa community, Tai L.G.A, Rivers State, an oil exploration zone of the Niger Delta, Nigeria which was later prepared for laboratory analyses. Nine (9) fungal isolates were screened for hydrocarbon biodegradation potentials in a shake-flask culture incorporated with 1% crude oil (hydrocarbon), redox reagent (2% 2,6-dichlorophenol indophenols) and 0.1 % Tween 80 for 14 days. Among the nine fungal isolates, eight showed potentials for hydrocarbon degradation employing colour change, optical density and THC for 14 days.  Five of the isolates exhibited the fastest onset and highest extent of biodegradation and were selected for further identification following polymerase chain reaction using 5'TCCGTAGGTGAACCTGCGG 3ʹ and 5ʹGCTGCGTTCTTCATCGATGC 3ʹ and were identified as: Aspergillus japonicus, Yarrowia lipolytica  YPY 01, Yarrowia lipolytica ATCC 9773, Zygorrhinchus sp.(1) and Zygorrhinchus sp.(2) respectively. In this study, it was observed that a higher THC biodegradation efficiency was exhibited by Yarrowia lipolytica ATCC 9773 and Zygorrhinchus sp.(2). This indicated that these fungal species are efficient hydrocarbon degraders. Thus, they can be considered in strain development programme for biodegradation of oil polluted farm lands (soil) especially those located within the sampled sites.

Keywords: Hydrocarbon, fungal species, Saakpenwa community

 

Related Articles


Original Research Articles
Tahany Mahmoud Refaat and Emad Girgis Kamel
Assessment of the impact of long exposure to cotton dust on respiratory health of workers in Minia city, Egypt
Glo. Adv. Res. J. Environ. Sci. Toxicol. March 2015 Vol: 4(3): - [Abstract] [Full Text - PDF] (122 KB)
Samuel Omorovie Akporido and Helen Ejiro Kadiri
Effect of Urbanization and Industrialization on Waters of the Benin River- Ethiope River System around Sapele, Nigeria
Glo. Adv. Res. J. Environ. Sci. Toxicol. August 2014 Vol: 3(3): - [Abstract] [Full Text - PDF] (1,815 KB)
Omar B. Ahmed and Bassam H. Mashat
Occurrence of ESBL, MRSA and VRE pathogens in contaminated banknotes in Makkah, Saudi Arabia.
Glo. Adv. Res. J. Environ. Sci. Toxicol. March 2015 Vol: 4(3): - [Abstract] [Full Text - PDF] (137 KB)
Adel S Al-Zubairi, Mohamed A Al-Mamary and Eftekhar Al-Ghasani
The Antibacterial, Antifungal, and Antioxidant Activities of Essential Oil from Different Aromatic Plants
Glo. Adv. Res. J. Environ. Sci. Toxicol. September 2017 Vol: 6(9): - [Abstract] [Full Text - PDF] (168 KB)
Imeh J Okop, Fiokedu S Okorie and Clement O Obadimu
Quantitative evaluation of the spatial distribution and penetration of liquid hydrocarbons in petroleum spilled soil
Glo. Adv. Res. J. Environ. Sci. Toxicol. September 2012 Vol: 1(6): - [Abstract] [Full Text - PDF] (1,307 KB)
Josephat KT, Kiiyukia C and Christine CB
Mycotoxigenicfungi, distribution and infestation of maize in selected sites- Kenya
Glo. Adv. Res. J. Environ. Sci. Toxicol. June 2015 Vol: 4(6): - [Abstract] [Full Text - PDF] (881 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Iheanacho CC on Google Scholar
Iheanacho CC on Pubmed
Okerentugba PO on Google Scholar
Okerentugba PO on Pubmed
Orji FA on Google Scholar
Orji FA on Pubmed
Ataikiru TL on Google Scholar
Ataikiru TL on Pubmed

Statistics

Viewed 1509
Printed 296
Downloaded 2521
Powered By iPortal Works