Global Advanced Research Journal of Environmental Science and Toxicology (GARJEST) ISSN: 2315-5140
September 2015 Vol. 4(3), pp. 025-030
Copyright © 2015 Global Advanced Research Journals

 

Full Length Research Paper

Detection of biofilm on surface using optical methods

Asha U. M. Lokuhewage

Sun Danuku International Co., Ltd, 387, Shirakuwa, Sakura-Ku, Saitama-Shi, Saitama-Ken, 338-0811

Email: uasha16@yahoo.com; Fax: +81-48-816-3623

Accepted 08 December, 2012

Abstract

The ability of many bacteria to adhere to surfaces and to form biofilms has major implications in a variety of industries and aquatic ecosystem, where biofilms create a persistent source of contamination. The formation of a biofilm is determined not only by the nature of the attachment surface, but also by the characteristics of the bacterial cell and by environmental factors. This review will focus on the bacterial development on surface, which is the interface of the bacterium with its surroundings, and on the properties of the attachment surface influencing biofilm formation. Optical Coherence Tomomgraphy (OCT) is an interferometric method that can provide two, or three dimensional in-vivo tomographic images of biofilm formation and development. The images revealed the depletion, changes in the density and area of distribution of the biofilm above the surface, and changes of the biofilm aggregate sizes.

Keywords: Optical Coherence Tomography, interferometric method

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