Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116

January, 2016. Vol. 5(1), pp 010-015

Copyright © 2016 Global Advanced Research Journals


Full Length Research Paper

Antibacterial evaluation and Phytochemical screening of Methanolic Extract of Ocimum sanctum against some common microbial pathogens 

Bilal Ahmad Tantry1, Avneesh Kumar2, Shiak Rahiman3, Mudasar Nabi Tantry4


1Department of Microbiology, College of Medicine, Al Jouf University, Saudi Arabia.

2 Institute of Applied Medicines & Research,  Ghaziabad, Uttar Pradesh, India.

3 Department of Biochemistry, College of Medicine, Al Jouf University, Saudi Arabia.

4 Department of clinical Biochemistry, Kashmir University, India.

*Corresponding Author’s Email:

Accepted 11 November, 2015



Whole plant methanolic extract of Ocimum sanctum was studied for their phytochemical and antibacterial activities. Antimicrobial activity of extract was detected against some common microbial pathogens. Methanolic extract of Ocimum sanctum exhibited antibacterial activity against Enterococcus faecalis, Enterobacter cloacae, Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae, Staphylococcus aureus and Staphylococcus saprophyticus. Methanolic extract of Ocimum sanctum showed strong antibacterial activity against Staphylococcus aureus and Staphylococcus saprophyticus. The minimal inhibitory concentrations for all tested strains were at concentrations ranging from 10.19±0.08mg/ml to 32.02±1.98 mg/ml. Phytochemical analysis of active extracts demonstrated the presence of common phytoconstituents like tannins, glycosides, saponins, flavonoids and alkaloids. These phytochemical established a good support to the use of this plant in herbal medicine and as a base for the development of new drugs, phytomedicine and might be aid in the treatment of UTI.

Keywords: Antimicrobial, indigenous medicine, Agar diffusion, UTI.


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