Global Advanced Research Journal of Microbiology (ISSN: 2315-5116) Vol. 4(11) pp. 130-139, December, 2015 Special Anniversary Review Issue

Copyright © 2015 Global Advanced Research Journals

 

Full Length Research Paper

Production and Optimization of Invertase From Pomegranate Peel by Marine Fungal Strain Aspergillus terreus

Aliaa R. El-Shamy and Sherien M.M. Atalla

 

Department of Chemistry of Natural and Microbial Products, National Research Centre, Giza, Egypt

*Corresponding Author’s Email: Aliaa.ragab@hotmail.com

Accepted 20 December, 2015

 

Abstract

Agricultural wastes were the low cost raw material for invertase production by fungi. The marine fungal strain Aspergillusterreus was  characterized and phylogenetic  analysis by 18S rRNA gene sequencing  then  evaluated for  invertase production using  40 g/l of pomegranate peel (Pp) as substrate. Maximum invertase activity was 264.4 U/ml after 5 days incubation period. Two disks 6mm in diameter, pH 6.5 and 30 C were the optimum conditions for invertase production. 3% of triton x100 as surfactant  produce 281.2 U/ml. Increasing invertase production by addition of wheat bran ,EDTA and  cysteine to pomegranate peel.

Keywords: marine fungi, invertase, pomegranate peel, optimization.


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