Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
February 2012 Vol. 1(1): pp. 001-004
Copyright © 2012 Global Advanced Research Journals


Full Length Research Paper


Antibacterial efficacy of crude and diluted honey on four wound isolates

W. Braide 1*, S.U.Oranusi1., C.K. Akaluka 1, R.N.Nwaoguikpe2., C.I. Akobundu1 and N.I. Peter-Ikechukwu3


1Department of Microbiology, Federal University of Technology, PMB 1526, Owerri, Imo State, Nigeria.

2Department of Biochemistry, Federal University of Technology, PMB 1526, Owerri, Imo State, Nigeria.

3Department of Food Science and Technology, Federal University of Technology, PMB 1526, Owerri, Imo State, Nigeria.


Accepted 05 February, 2012



The antimicrobials activities of crude and diluted honey were determined against four clinical isolates from surface and deep wounds. Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli were isolated from wounds by routine microbiological methods. Kirby- Bauer’s disc diffusion method was employed in determining the susceptibility of bacterial isolates to different concentrations of honey. The results from the zone of inhibition obtained (in mm) showed that the growth of all isolates was completely inhibited by 20-100% honey concentrations. The minimum inhibitory concentrations (MIC) of honey for P. aeruginosa and S. aureus were at 10 % (v/v) while that of E.coli and K. pneumoniae were 20% (v/v). The degree of susceptibility of the wound isolates to honey was compared with that of ten commercially available antibiotic discs. The result obtained revealed that the susceptibility pattern of honey at 40% (v/v) compared favourably and better than amoxicillin, streptomycin, ceftriazone and erythromycin. 

Keywords:  Honey, antibacterial activities, wound bacteria.



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