Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116

February, 2016. Vol. 5(2), pp 016-022

Copyright © 2016 Global Advanced Research Journals

Full Length Research Paper

Antagonistic bacteria affecting the Golden cyst potato nematode (Globoderarostochiensis Woll.) in the region of Perote, Veracruz, México

Salinas-Castro A¹, Nava-Díaz C³, Luna- Rodríguez M², San Martín-Romero E², Rivera-Fernández A*¹, Trigos A²

¹Facultad de Ciencias Agrícolas, Universidad Veracruzana, Zona Universitaria s/n, Xalapa, Veracruz, México CP 91000.

²Laboratorio de Alta Tecnología de Xalapa S.C., Universidad Veracruzana, Médicos No. 5, Unidad del Bosque, Xalapa, Veracruz, México. CP.91010.

³Colegio de Posgraduados Carretera México-Texcoco Km. 36.5, Montecillo, Texcoco CP.56230 Estado de México.

*Corresponding Author’s Email: arivera@uv.mx

Accepted 26 February, 2016

Abstract

The golden cyst nematode (Globoderarostochiensis) presents one of the major problems in potato farming in highlandregionsin variousparts of the world and México is no exception. The high application of chemicals to control the nematode and the related environmental problems have led to the search for alternative control options, such as the use of rhizobacteria, which have indicatednew ways of achieving an increase in agricultural productivity as well asbeing an ecological and economically feasible means to minimize harmful practices in agriculture. The goal of this study was to isolate and evaluate "in vitro " the antagonistic capacity of rhizobacteria affecting cysts and J2 stages of Globoderarostochiensis.  Nematodes were extracted from soil samples from the localities of Perote and Los Altos de Ayahualulco and identified morphometric and molecularly. Potato roots from undisturbed areas in the highland region of Perote were collected for bacterial isolation.The bacteria were isolated on media King B, MB and nutrient agar, and were identified byananalysis ofthe 16S rRNA subunit gene. 102 bacterial isolates were evaluated on J2 and G. rostochiensis cysts. Ten of the bacterial isolates were seen to cause distortion and disintegration of J2 nematode individuals. None of the bacterial isolates caused damage in cysts. The cysts provide the female with a defense strategy, which makes them difficult to control. We found that promising bacterial species corresponded to the generas Providencia, Pseudomonas, Paracoccus and Serratia.

Keywords: Microbial competition, biocontrol, plant parasitic nematodes, rhizobacteria.