Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
February 2018 Vol. 7(1): pp. 006-022
Copyright © 2018 Global Advanced Research Journals


Full Length Research Paper


Bioremediation of Methyl Orange onto Nostoc carneum Biomass by Adsorption; Kinetics and Isotherm Studies

Mervat H. Hussein*, Ghada S. Abou El-Wafa, Sami A. Shaaban-Dessuki and Rehab M. El-Morsy


Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt


Accepted 08 February, 2018



The present study is concerning with the use of cyanobacterium Nostoc carneum as a model for potential biosorbent of azo dye Methyl Orange (MO) from aquatic solutions. The impacts of major variables (pH, contact time, initial dye concentration, biosorbent capacity and salt concentrations) overriding algal biosorbent and process settings on specific decolorization rate and dye biosorbtion were investigated. Michaelis–Menten kinetics model was applied to assess the decolorization kinetics factors as 2.173 mg g-1 h-1 and 46.435 mg L−1 for maximum specific decolorization rate and km of dye concentration, respectively. The adsorption of MO is increasing with increasing the initial dye concentration as well as the algal biosorbent. Nostoc carneum with decolorization efficiency 50.749, 44.225, 42.934, 38.367, 36.211, 28.056, 23.754, 18.273 and 17.241 % at dye concentrations of 5, 10, 20, 30, 40, 50, 60, 70 and 80 mg L−1, respectively at contact time 5 h. The maximum specific decolorization rate was found to be 2.1734 mg g-1 h-1. Pseudo-second order kinetic rate and Langmuir adsorption isotherm models were the best fitted with the experimental equilibrium data. The interaction of MO with the biosorbent was demonstrated on the bases of scanning electron microscopy (SEM), FT-IR and XRD spectral data that prove the efficiency of Nostoc carneum fresh biomass with respect to alternative low-cost technology for azo dyes bioremediation. . 

Keywords:  Nostoc carneum; Azo dye; Methyl orange; Biosorption kinetics; Isotherms; SEM; XRD; FTIR.





Ahmad R, Kumar R (2010). Adsorption studies of hazardous malachite green onto treated ginger waste. Journal of environmental management. 91: 1032-1038.

Aksu Z, Tezer S (2005). Biosorption of reactive dyes on the green alga Chlorella vulgaris. Process biochemistry. 40: 1347-1361.

Azizian S (2004). Kinetic models of sorption: a theoretical analysis. Journal of colloid and Interface Science. 276: 47-52.

Çelekli A, Tanrıverdi B, Bozkurt H (2011). Predictive modeling of removal of Lanaset Red G on Chara contraria; kinetic, equilibrium, and thermodynamic studies. Chemical engineering journal. 169: 166-172.

Çelekli A, İlgün G, Bozkurt H (2012). Sorption equilibrium, kinetic, thermodynamic, and desorption studies of Reactive Red 120 on Chara contraria. Chemical engineering journal. 191: 228-235.

Chandrasekhar S, Pramada P (2006). Rice husk ash as an adsorbent for methylene blue—effect of ashing temperature. Adsorption. 12: 27-43.

Chen J-P, Lin Y-S (2007). Decolorization of azo dye by immobilized Pseudomonas luteola entrapped in alginate–silicate sol–gel beads. Process biochemistry. 42: 934-942.

Chieng HI, Priyantha N, Lim LB (2015). Effective adsorption of toxic brilliant green from aqueous solution using peat of Brunei Darussalam: isotherms, thermodynamics, kinetics and regeneration studies. RSC Advances. 5: 34603-34615.

Chojnacka K, Chojnacki A, Gorecka H (2005). Biosorption of Cr 3+, Cd 2+ and Cu 2+ ions by blue–green algae Spirulina sp.: kinetics, equilibrium and the mechanism of the process. Chemosphere. 59: 75-84.

Chu W-L, See Y-C, Phang S-M (2009). Use of immobilised Chlorella vulgaris for the removal of colour from textile dyes. Journal of Applied Phycology. 21: 641.

Crist RH, Oberholser K, Shank N, Nguyen M (1981). Nature of bonding between metallic ions and algal cell walls. Environmental Science & Technology. 15: 1212-1217.

Daneshvar N, Ayazloo M, Khataee A, Pourhassan M (2007). Biological decolorization of dye solution containing  Malachite Green by microalgae Cosmarium sp. Bioresource technology. 98: 1176-1182.

El-Sheekh MM, Gharieb M, Abou-El-Souod G (2009). Biodegradation of dyes by some green algae and cyanobacteria. International Biodeterioration & Biodegradation. 63: 699-704.

Fakhry EM (2013). Padina pavonica for the removal of dye from polluted water. American Journal of Plant Sciences. 4: 1983.

Gong R, Jin Y, Chen J, Hu Y, Sun J (2007). Removal of basic dyes from aqueous solution by sorption on phosphoric acid modified rice straw. Dyes and Pigments. 73: 332-337.

Heiss GS, Gowan B, Dabbs ER (1992). Cloning of DNA from a Rhodococcus strain conferring the ability to decolorize sulfonated azo dyes. FEMS microbiology letters. 99: 221-226.

Hernández-Zamora M, Cristiani-Urbina E, Martínez-Jerónimo F, Perales-Vela HV, Ponce-Noyola T, del Carmen Montes-Horcasitas M, Cañizares-Villanueva RO (2015). Bioremoval of the azo dye Congo Red by the microalga Chlorella vulgaris. Environmental Science and Pollution Research. 22: 10811-10823.

Ho Y-S (2006). Second-order kinetic model for the sorption of cadmium onto tree fern: a comparison of linear and non-linear methods. Water Research. 40: 119-125.

Hsueh C-C, Chen B-Y, Yen C-Y (2009). Understanding effects of chemical structure on azo dye decolorization characteristics by Aeromonas hydrophila. Journal of hazardous materials. 167: 995-1001.

Jinqi L, Houtian L (1992). Degradation of azo dyes by algae. Environmental pollution. 75: 273-278.

Kam S-K, Gregory J (2001). The interaction of humic substances with cationic polyelectrolytes. Water Research. 35: 3557-3566.

Kelewou H, Merzouki M, Lhassani A (2014). Biosorption of textile dyes Basic Yellow 2 (BY2) and Basic Green 4 (BG4) by the live yeast Saccharomyces Cerevisiae. J. Mater. Environ. 5: 633-640.

Kiran B, Rani N, Kaushik A (2016). FTIR spectroscopy and scanning electron microscopic analysis of pretreated biosorbent to observe the effect on Cr (VI) remediation. International journal of phytoremediation. 18: 1067-1074.

Kodam K, Soojhawon I, Lokhande P, Gawai K (2005). Microbial decolorization of reactive azo dyes under aerobic conditions. World Journal of Microbiology and Biotechnology. 21: 367-370.

Kumar R, Ahmad R (2011). Biosorption of hazardous crystal violet dye from aqueous solution onto treated ginger waste (TGW). Desalination. 265: 112-118.

Kyzas GZ, Lazaridis NK, Mitropoulos AC (2012). Removal of dyes from aqueous solutions with untreated coffee residues as potential low-cost adsorbents: Equilibrium, reuse and thermodynamic approach. Chemical engineering journal. 189: 148-159.

Langmuir I (1918). The adsorption of gases on plane surfaces of glass, mica and platinum. Journal of the American Chemical society. 40: 1361-1403.

Mahmoud MS, Mostafa MK, Mohamed SA, Sobhy NA, Nasr M (2017). Bioremediation of red azo dye from aqueous solutions by Aspergillus niger strain isolated from textile wastewater. Journal of Environmental Chemical Engineering. 5: 547-554.

Malik PK (2003). Use of activated carbons prepared from sawdust and rice-husk for adsorption of acid dyes: a case study of Acid Yellow 36. Dyes and Pigments. 56: 239-249.

Mane VS, Babu PV (2011). Studies on the adsorption of Brilliant Green dye from aqueous solution onto low-cost NaOH treated saw dust. Desalination. 273: 321-329.

Mohan SV, Bhaskar YV, Karthikeyan J (2004). Biological decolourisation of simulated azo dye in aqueous phase by algae Spirogyra species. International journal of environment and pollution. 21: 211-222.

Mohanty K, Jha M, Meikap B, Biswas M (2006). Biosorption of Cr (VI) from aqueous solutions by Eichhornia crassipes. Chemical engineering journal. 117: 71-77.

Mona S, Kaushik A, Kaushik C (2011). Waste biomass of Nostoc linckia as adsorbent of crystal violet dye: optimization based on statistical model. International Biodeterioration & Biodegradation. 65: 513-521.

Murphy V, Hughes H, McLoughlin P (2007). Cu (II) binding by dried biomass of red, green and brown macroalgae. Water Research. 41: 731-740.

Namasivayam C, Kavitha D (2006). IR, XRD and SEM studies on the mechanism of adsorption of dyes and phenols by coir pith carbon from aqueous phase. Microchemical Journal. 82: 43-48.

Nandi B, Goswami A, Purkait M (2009). Removal of cationic dyes from aqueous solutions by kaolin: kinetic and equilibrium studies. Applied Clay Science. 42: 583-590.

Natarajan E, Ponnaiah GP (2017). Optimization of process parameters for the decolorization of Reactive Blue 235 dye by barium alginate immobilized iron nanoparticles synthesized from aluminum industry waste. Environmental Nanotechnology, Monitoring & Management. 7: 73-88.

Noreen S, Bhatti HN, Nausheen S, Sadaf S, Ashfaq M (2013). Batch and fixed bed adsorption study for the removal of Drimarine Black CL-B dye from aqueous solution using a lignocellulosic waste: A cost affective adsorbent. Industrial Crops and Products. 50: 568-579.

Omar HH (2008). Algal decolorization and degradation of monoazo and diazo dyes. Pak J Biol Sci. 11: 1310-1316.

Ong S-A, Uchiyama K, Inadama D, Ishida Y, Yamagiwa K (2010). Treatment of azo dye Acid Orange 7 containing wastewater using up-flow constructed wetland with and without supplementary aeration. Bioresource technology. 101: 9049-9057.

Pearce C, Lloyd J, Guthrie J (2003). The removal of colour from textile wastewater using whole bacterial cells: a review. Dyes and Pigments. 58: 179-196.

Pinheiro H, Touraud E, Thomas O (2004). Aromatic amines from azo dye reduction: status review with emphasis on direct UV spectrophotometric detection in textile industry wastewaters. Dyes and Pigments. 61: 121-139.

Pivokonsky M, Naceradska J, Kopecka I, Baresova M, Jefferson B, Li X, Henderson R (2016). The impact of algogenic organic matter on water treatment plant operation and water quality: A review. Critical Reviews in Environmental Science and Technology. 46: 291-335.

Rakhshaee R, Giahi M, Pourahmad A (2011). Removal of methyl orange from aqueous solution by Azolla filicoloides: synthesis of Fe 3 O 4 nano-particles and its surface modification by the extracted pectin of Azolla. Chinese Chemical Letters. 22: 501-504.

Rau J, Stolz A (2003). Oxygen-insensitive nitroreductases NfsA and NfsB of Escherichia coli function under anaerobic conditions as lawsone-dependent azo reductases. Applied and Environmental Microbiology. 69: 3448-3455.

Safa Y, Bhatti HN (2011). Kinetic and thermodynamic modeling for the removal of Direct Red-31 and Direct Orange-26 dyes from aqueous solutions by rice husk. Desalination. 272: 313-322.

Sarı A, Tuzen M (2008). Biosorption of cadmium (II) from aqueous solution by red algae (Ceramium virgatum): equilibrium, kinetic and thermodynamic studies. Journal of hazardous materials. 157: 448-454.

Şeker A, Shahwan T, Eroğlu AE, Yılmaz S, Demirel Z, Dalay MC (2008). Equilibrium, thermodynamic and kinetic studies for the biosorption of aqueous lead (II), cadmium (II) and nickel (II) ions on Spirulina platensis. Journal of hazardous materials. 154: 973-980.

Shah M, Patel K, Nair S, Darji A (2013). Microbial decolourization of methyl orange dye by Pseudomonas spp. OA Biotechnology. 2: 10.

Shroff KA, Vaidya VK (2011). Kinetics and equilibrium studies on biosorption of nickel from aqueous solution by dead fungal biomass of Mucor hiemalis. Chemical engineering journal. 171: 1234-1245.

Srivastava R, Rupainwar D (2009). Eucalyptus bark powder as an effective adsorbent: Evaluation of adsorptive characteristics for various dyes. Desalination and Water Treatment. 11: 302-313.

Stanier R, Kunisawa R, Mandel M, Cohen-Bazire G (1971). Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriological reviews. 35: 171.

Suart B (2004). Infrared Spectroscopy: Fundamental and Applications. John Wiley & Sons, Ltd.

Tan C-y, Li G, Lu X-Q, Chen Z-l (2010). Biosorption of Basic Orange using dried A. filiculoides. Ecological Engineering. 36: 1333-1340.

Tsai W-T, Chen H-R (2010). Removal of malachite green from aqueous solution using low-cost chlorella-based biomass. Journal of hazardous materials. 175: 844-849.

Vijayaraghavan K, Padmesh T, Palanivelu K, Velan M (2006). Biosorption of nickel (II) ions onto Sargassum wightii: application of two-parameter and three-parameter isotherm models. Journal of hazardous materials. 133: 304-308.

Wang Y, Ho S-H, Cheng C-L, Guo W-Q, Nagarajan D, Ren N-Q, Lee D-J, Chang J-S (2016). Perspectives on the feasibility of using microalgae for industrial wastewater treatment. Bioresource technology. 222: 485-497.

Waranusantigul P, Pokethitiyook P, Kruatrachue M, Upatham E (2003). Kinetics of basic dye (methylene blue) biosorption by giant duckweed (Spirodela polyrrhiza). Environmental pollution. 125: 385-392.

Weber TW, Chakravorti RK (1974). Pore and solid diffusion models for fixed‐bed adsorbers. AIChE Journal. 20: 228-238.

Yadav S, Srivastava V, Banerjee S, Weng C-H, Sharma YC (2013). Adsorption characteristics of modified sand for the removal of hexavalent chromium ions from aqueous solutions: Kinetic, thermodynamic and equilibrium studies. Catena. 100: 120-127.










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