Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
February 2018 Vol. 7(1): pp. 023-036
Copyright © 2018 Global Advanced Research Journals

 

Full Length Research Paper

 

Molecular identification of microorganisms in chronic wounds, Republic of Guinea (Conakry)

Cyrille Bilé Ehounoud1,2, Alpha Kabinet Keita3, Abdoul Habib Béavogui4,5, Ansoumane Cissé4, Nadia Amanzougaghene1, Jean David N’Guessan2, Didier Raoult1, Oleg Mediannikov1, Florence Fenollar1*

 

1Aix Marseille Univ, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198, INSERM 1095, IHU - Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille

2Felix Houphouet Boigny Université, UFR Biosciences, Côte D’Ivoire.

3Unité de recherche translationnelle appliquée au VIH et les maladies infectieuses (TransVIHMI) IRD UMI 233-INSERM U 1175 Centre IRD France Sud 911, avenue Acropoles, BP 64501 F-34394 Montpellier cedex 5.

4Centre de Formation et de Recherche en Santé Rurale de Mafèrinyah B.P. 2649, Conakry, République de Guinée.

5Faculté de Médecine, Pharmacie et Odonto-Stomatologie, Université Gamal Abdel Nasser de Conakry, République de Guinée.

Email: florence.fenollar@univ-amu.fr

Accepted 13 January, 2018

 

Abstract

Skin infections are common in sub-Saharan Africa, including chronic wounds. This study aimed to assess the presence of several microorganisms in skin specimens from patients with chronic wounds and healthy people in Maferinyah (Republic of Guinea). Eighty-four skin samples from the wounds of 20 patients (42 edge swabs and 42 center swabs) and twenty-two skin samples from 11 healthy people were analyzed by real-time quantitative PCR and standard PCR assays combined with sequencing. Pseudomonas aeruginosa was the most frequently detected bacterium, which was significantly more prevalent in patients (80%, 16/20) than in healthy people (9%, 1/11; p<0.001), followed by Staphylococcus aureus which was only detected in patients (60%, 12/20; p<0.001). Streptococcus pyogenes was also more frequently detected in patients (30%, 6/20) than in healthy people (9%, 1/11) but the difference was not statistical significant. Rickettsia felis was also detected for the first time in Guinea, in one patient. Finally, species of the genus Acinetobacter were also frequently and exclusively detected in patients (80%, 16/20). Acinetobacter baumannii (2/20, 10%), Acinetobacter nosocomialis (10%), Acinetobacter junii (1/20, 5%), Acinetobacter lwofii (5%), and Acinetobacter guangdongensis (5%), which was detected for the first time in skin, were identified. Acinetobacter junii and Acinetobacter lwofii were observed in different samples from the same patient. For the 11 other patients, polymicrobial infections featuring several species of the genus Acinetobacter were observed.  Overall, many different bacteria which may encourage wound enlargement or delayed healing were observed in chronic wounds.

Keywords:  skin; chronic wound; bacteria; Staphylococcus aureus; Pseudomonas aeruginosa; Acinetobacter spp.; Rickettsia felis; Guinea (Conakry).

 

 

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