Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116

April, 2016. Vol. 5(3), pp 023-031

Copyright © 2016 Global Advanced Research Journals

 

Full Length Research Paper

Isolation of Rhizobacteria and Micro Algae from Saline Soil and Production of Plant   Growth Promoters

Shimaa E. Ibrahim *, Entesar A. Ahmed *, Heba Sh. Shehata **, Reda M. El Shahat ** and Rawheya A. Salah El Din * 

 

*Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt. 

**Microbiology Department, Soils; Water and Environment Research Institute, Agriculture Research Center, Giza, Egypt.

*Corresponding Author’s Email: elmonaliza96@gmail.com

Accepted 10 April, 2016

 

Abstract

Twenty-six bacterial and four algal isolates were isolated from rhizosphere of rice and sugar beet plants, grown in saline soil in Sahl El-Hussinian Station, El-Sharkia Governorate, Egypt and in vitro screening was done for different plant growth promotion activities i. e. indole acetic acid (IAA), gibberellic acid (GA) and carbohydrate production. Bio-control ability of bacterial isolates was screened by antagonistic activity against certain fungal pathogens. Results revealed that bacterial isolates No. 7 and 26 produced high amount of IAA (78.63 µg ml-1 and 78.73 µg ml-1, respectively), while isolate No. 5 was most potent isolate for GA (173.91 µg ml-1) and carbohydrate (187.18 µg ml-1) production.  The active bacterial isolates (No. 5, 7, 26) in plant growth promoting substances production showed high antagonistic activity against certain pathogenic fungi (Fusarium sp. and Rhizoctonia sp.). Additionally, algal isolate No. 3 was active in production IAA (29.26 µg ml-1), while isolates No. 2 and 1 were efficient in GA (83.91 µg ml-1) and carbohydrate (178.81 µg ml-1) production, respectively. The most efficient rhizobacterial isolates (No. 5, 7 and 26) were selected and identified using Bio-log technique and 16S rRNA as Bacillus subtilis and Pseudomonas aeruginosa, respectively. Based on 16S rRNA sequence analysis both bacterial isolates (No. 7 and 26) identified as Pseudomonas aeruginosa. The most potent algal isolates (No. 1, 2 and 3) for plant growth promoting substances production were chosen and identified according to classical morphological characters as Anabaena sp., Nostoc muscorum and Anabaena variabilis, respectively. The optimum incubation period for highest production of IAA and GA by efficient bacterial and algal isolates was three and twenty days, respectively, while highest carbohydrate production was obtained after four and twenty five days, respectively. 

Keywords: Soil salinity, Isolation, Purification, Identification, Phytohormones, Carbohydrate, Biocontrol, incubation periods.

 


Related Articles


Original Research Articles
Dina El-Kahky, Magdy Attia, Saadia M Easa, Nemat M. Awad and Eman A. Helmy
Biosynthesized of zinc oxide nanoparticles using Aspergillus terreus and their application as antitumor and antimicrobial activity
Glo. Adv. Res. J. Microbiol March 2019 Vol: 8(3): - [Abstract] [Full Text - PDF] (1,543 KB)
Saleh A S AL-Abdulhadi
Spotlight into some Abnormalities of Human Chromosome in Saudi Arabia
Glo. Adv. Res. J. Microbiol January 2018 Vol: 7(1): - [Abstract] [Full Text - PDF] (257 KB)
Alamu, E. Oladeji, Maziya-Dixon Bussie, Menkir Abebe, Olaofe Olorunfemi, Irondi, Emmanuel A
Effect of Maturity Stages and Roasting Method on the Proximate Composition of orange Maize Hybrids
Glo. Adv. Res. J. Microbiol August 2015 Vol: 4(8): - [Abstract] [Full Text - PDF] (148 KB)
Review
Innocent Chirisa and Halleluah Mangombe-Chirisa
Adapting to pressure? Social capital dynamics in Greendale Harare
Glo. Adv. Res. J. Microbiol July 2012 Vol: 1(2): - [Abstract] [Full Text - PDF] (420 KB)
Original Research Articles
Afaf S. Altalhi; Aziza M. Hassan; Shatha Dhaifallah Al-Harthi and Fatima Sulaiman Al-harbi
Wild Olive Leaf Extracts Up regulate Insulin-signaling Gene Expression in Diabetic mice
Glo. Adv. Res. J. Microbiol April 2018 Vol: 7(4): - [Abstract] [Full Text - PDF] (281 KB)
P. Azinwi Tamfuh, E. Djoufac Woumfo, F.O. Tabi, M. Angue-Abossolo and D. Bitom
Geostatistical modeling and agricultural perspectives of vertisol properties in the Benue Floodplain of North Cameroon
Glo. Adv. Res. J. Microbiol July 2018 Vol: 7(7): - [Abstract] [Full Text - PDF] (1,736 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Shimaa E. Ibrahim on Google Scholar
Shimaa E. Ibrahim on Pubmed
Entesar A. Ahmed on Google Scholar
Entesar A. Ahmed on Pubmed
Heba Sh. Shehata on Google Scholar
Heba Sh. Shehata on Pubmed
Reda M. El Shahat on Google Scholar
Reda M. El Shahat on Pubmed
Rawheya A. Salah El Din on Google Scholar
Rawheya A. Salah El Din on Pubmed

Statistics

Viewed 2364
Printed 379
Downloaded 1487
Powered By iPortal Works