Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116

April, 2016. Vol. 5(3), pp 023-031

Copyright © 2016 Global Advanced Research Journals

 

Full Length Research Paper

Isolation of Rhizobacteria and Micro Algae from Saline Soil and Production of Plant   Growth Promoters

Shimaa E. Ibrahim *, Entesar A. Ahmed *, Heba Sh. Shehata **, Reda M. El Shahat ** and Rawheya A. Salah El Din * 

 

*Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt. 

**Microbiology Department, Soils; Water and Environment Research Institute, Agriculture Research Center, Giza, Egypt.

*Corresponding Author’s Email: elmonaliza96@gmail.com

Accepted 10 April, 2016

 

Abstract

Twenty-six bacterial and four algal isolates were isolated from rhizosphere of rice and sugar beet plants, grown in saline soil in Sahl El-Hussinian Station, El-Sharkia Governorate, Egypt and in vitro screening was done for different plant growth promotion activities i. e. indole acetic acid (IAA), gibberellic acid (GA) and carbohydrate production. Bio-control ability of bacterial isolates was screened by antagonistic activity against certain fungal pathogens. Results revealed that bacterial isolates No. 7 and 26 produced high amount of IAA (78.63 µg ml-1 and 78.73 µg ml-1, respectively), while isolate No. 5 was most potent isolate for GA (173.91 µg ml-1) and carbohydrate (187.18 µg ml-1) production.  The active bacterial isolates (No. 5, 7, 26) in plant growth promoting substances production showed high antagonistic activity against certain pathogenic fungi (Fusarium sp. and Rhizoctonia sp.). Additionally, algal isolate No. 3 was active in production IAA (29.26 µg ml-1), while isolates No. 2 and 1 were efficient in GA (83.91 µg ml-1) and carbohydrate (178.81 µg ml-1) production, respectively. The most efficient rhizobacterial isolates (No. 5, 7 and 26) were selected and identified using Bio-log technique and 16S rRNA as Bacillus subtilis and Pseudomonas aeruginosa, respectively. Based on 16S rRNA sequence analysis both bacterial isolates (No. 7 and 26) identified as Pseudomonas aeruginosa. The most potent algal isolates (No. 1, 2 and 3) for plant growth promoting substances production were chosen and identified according to classical morphological characters as Anabaena sp., Nostoc muscorum and Anabaena variabilis, respectively. The optimum incubation period for highest production of IAA and GA by efficient bacterial and algal isolates was three and twenty days, respectively, while highest carbohydrate production was obtained after four and twenty five days, respectively. 

Keywords: Soil salinity, Isolation, Purification, Identification, Phytohormones, Carbohydrate, Biocontrol, incubation periods.

 


Related Articles


Original Research Articles
Ashraful Kabir M
Karyotypic analysis of chickens and other birds
Glo. Adv. Res. J. Microbiol September 2012 Vol: 1(2): - [Abstract] [Full Text - PDF] (611 KB)
Selva Prabhu A, Ananthan G, And Bala Subramanian T
First Record of Mitochondrial Cytochrome Oxidase I gene sequences of Ascidian Polyclinum madrasensis (Sebestian, 1952) from Gulf of Mannar, Southeast coast of India
Glo. Adv. Res. J. Microbiol September 2012 Vol: 1(2): - [Abstract] [Full Text - PDF] (148 KB)
Mohamed T. Shaaban, M. Attia, Eman I. Mowafy, Azza Sh. Turky and Nemat M. Awad
Identification and Improving Antibiotic Production by Some Bacteria Isolated From Egyptian Soil
Glo. Adv. Res. J. Microbiol September 2015 Vol: 4(9): - [Abstract] [Full Text - PDF] (1,296 KB)
Kinsiclounon Gilles E, Edorh Patrick A, Guedenon Patient, Senou Masimin, Deguenon Yvette, Akpovi Casimir, Kaki Christophe Loko Frederic, Boko Michel, Gbeassor Messanvi and Creppy Edmond E
Comparative evaluation of lead, cadmium, nitrates, total iron and their derivatives, in drinking water in the lacustrine township of Sô-Ava between 2009 and 2012
Glo. Adv. Res. J. Microbiol September 2013 Vol: 2(7): - [Abstract] [Full Text - PDF] (448 KB)
Ayat A Sayed, Sahar EM EL-Deek, Mona A EL-Baz, Dina Sabry, Aliaa Al-Rageaey, Aishaa Mansey, Fatma Y Meligy and Khaled Abdelaziz
Exosomes Derived from Bone Marrow Mesenchymal Stem Cells Restore Cisplatin Induced Ovarian Damage by Promoting Stem Cell Survival, Meiotic, and Apoptotic Markers
Glo. Adv. Res. J. Microbiol June 2017 Vol: 6(6): - [Abstract] [Full Text - PDF] (5,455 KB)
Josephat KT, Kiiyukia C and Christine CB
Mycotoxigenicfungi, distribution and infestation of maize in selected sites- Kenya
Glo. Adv. Res. J. Microbiol June 2015 Vol: 4(6): - [Abstract] [Full Text - PDF] (881 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Shimaa E. Ibrahim on Google Scholar
Shimaa E. Ibrahim on Pubmed
Entesar A. Ahmed on Google Scholar
Entesar A. Ahmed on Pubmed
Heba Sh. Shehata on Google Scholar
Heba Sh. Shehata on Pubmed
Reda M. El Shahat on Google Scholar
Reda M. El Shahat on Pubmed
Rawheya A. Salah El Din on Google Scholar
Rawheya A. Salah El Din on Pubmed

Statistics

Viewed 1805
Printed 334
Downloaded 1263
Powered By iPortal Works