Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
April 2018 Vol. 7(2): pp. 040-047
Copyright © 2018 Global Advanced Research Journals
Full Length Research Paper
Molecular Characterization of CTX-M ESBLs among Pathogenic Enterobacteriaceae isolated from different regions in Sudan
Hisham N Altayb1*, Mohamed A M Siddig2, Nagwa M El Amin3, Ahmed Ibrahim Hashim1, Maowia M. Mukhtar4
1College of medical laboratory science, Sudan University of science and technology, Sudan.
2Botany department, Faculty of Science, University of Khartoum, Sudan
3Department of microbiology, Faculty of medicine, university of Khartoum, Sudan
4Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan
Accepted 07 April, 2018
This is a cross sectional study conducted to detect and characterize CTX-M genes among extended spectrum β-lactamases (ESBLs) producing Enterobacteriaceae isolated from different regions in Sudan. A total of 305 of clinical Enterobacteriaceae sp. isolates were collected from different regions in Sudan. ESBLs production was initially screened by cefotaxime, cefepime and ceftazidime, and then confirmed by disk combination method and PCR. DNA sequencing was done to differentiate between blaCTX-M genotypes. Escherichia coli was the most predominant (58%) isolate, followed by Klebsiella pneumoniae (26.6%), Citrobacter freundii (3.6%), Enterobacter species (6.2%) and Proteus species (5.6%). ESBLs were detected by disk combination method in 128 (42%) isolates; Khartoum state 64% (23/36), Gizera state 54% (54/100), Sinnar state 53% (49/92) and White Nile state 2.6% (2/77). Three quarters of the ESBLs producers (96/128) were positive for blaCTX-M genes by PCR. blaCTX-M-15 was the most reported one 78.3% (18/23), followed by blaCTX-M-14 13% (3/23), blaCTX-M-27 4.3% (1/23) and blaCTX-M-98 4.3% (1/23). There was a transition mutation (substitution of A with G at position 25) in the blaCTX-M gene (ID: KP309815), that affect protein structure. We concluded that blaCTX-M-15 was the most commonly encountered genes and widely spread in different Sudanese regions.
Keywords: blaCTX-M; Beta-Lactamases Genes; Enterobacteriaceae; Sudan.
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- Hisham N Altayb on Google Scholar
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- Mohamed A M Siddig on Google Scholar
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- Nagwa M El Amin on Google Scholar
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- Ahmed Ibrahim Hashim on Google Scholar
- Ahmed Ibrahim Hashim on Pubmed
- Maowia M. Mukhtar on Google Scholar
- Maowia M. Mukhtar on Pubmed