Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116

May, 2015. Vol. 4(4), pp 036-049

Copyright © 2015 Global Advanced Research Journals

Full Length Research Paper

Biosynthesis of Silver Nanoparticles By Spirulina platensis & Nostoc sp.

Entesar A. Ahmed⁽¹⁾, Ekbal H. Abdel Hafez², Amel F. M. Ismail⁽³⁾ Sawsan M. Elsonbaty⁽⁴⁾, and Heba* S. Abbas⁽⁵⁾ , Rawheya A. Salah El Din⁽⁶⁾

(1) Lecturer of Phycology Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girl Branch).

(2) Lecturer of Microbiology - National Organization for Drug Control and Research (NODCAR)

(3) Lecturer of Biochemistry - Drug Radiation Research Department(NCRRT)

(4) Assistant professor of Biochemistry Microbiology (NCRRT).

(5) PhD Student Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girl Branch) and Drug control specialist at (NODCAR)

(6) Professor of Phycology, Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girl Branch).

*Corresponding Author Email: Heba181179@yahoo.com

Accepted 11 May, 2015

Abstract

Synthesis of nanoparticles that have environmentally acceptable solvent systems and eco-friendly reducing agents is of great importance. The aim of this work was to synthesis of silver nanoparticles (AgNPs) using Spirulina platensis and Nostoc sp. at room temperature. UV–visible spectrometry study revealed surface plasmon resonance at 402 nm. The transmission electron micrographs of nanoparticles in an aqueous solution showed the production of AgNPs (average size of most particles_˜11.5 nm by Spirulina platensis and 20.3 nm by Nostoc sp.). The Fourier Transmittance Infrared spectrum (FT-IR) confirms the presence of biocomponent in the algae which was responsible for the nanoparticles synthesis. The antibacterial activity silver nanoparticles against both gram positive bacteria (Staphylococcus aureus, Staphylococcus epidedymis) and gram negative bacteria (Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa) by well diffusion method showed excellent antibacterial activity against all tested bacterial. This green process gives the greater potential biomedical applications of silver nanoparticles.

Keywords: Biogenic nanoparticles, silver nanoparticles, algae, Antimicrobial Activity of Silver nanoparticles.