Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
May 2018 Vol. 7(3): pp. 057-063
Copyright © 2018 Global Advanced Research Journals

 

Full Length Research Paper

 

Genetic mutagenesis through Transposable element 5 (Tn5) to improve beta-D-galactosidase productivity from different bacterial strains

Hind A. A. Al-Zahrani

 

Biology Depart., Faculty of Sciences, University of Jeddah, Jeddah, Saudi 

Email: nognagy@yahoo.com

 

Accepted 13 May, 2018

 

Abstract

β-galactosidaseIs an enzyme that catalyzes the hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides, Suicide plasmids considered a good genetic tool for DNA mutagenesis in bacterial origin specially gram negative ones, in this study a group of suicide plasmids carrying transposal element genes Tn5 and Tn7 were used for Beta-D-galactosidase productivity improvement in Escherichia coli strains through mutagenesis stimulation in genomic DNA of recipient cells for plasmid, these plasmids transferred through transconjugation mechanism as both strains related at species level. The results showed that beta-galactosidase productivity was improved in the trans-conjugated isolates which analyzed at DNA level to detect inserted plasmid through beta-galactosidase coding gene carried on it.   

Keywords:  β-galactosidase, enzyme, catalyzes, hydrolysis, non-reducing β-D-galactose.

 

 

REFERENCES

 

Bertani G (1951). Studies on lysogenesis. I. The mode of phage liberation by lysogenic  Esherichia coli . J. Bacteriol., 62: 293-300.

Carla Oliveira, Pedro MR, Guimarães MR, Lucília D (2011). Recombinant microbial systems for improved β-galactosidase production and biotechnological applications. Biotechnology Advances 29: 600–609.

Gaur, R., H. Pant H, R. Jain R, and S. K. Khare SK, (2006). Galacto-oligosaccharide synthesis by immobilized Aspergillusoryzae β-galactosidase. Food Chemistry, 97(3): 426-430.

Gosling A, Stevens GW, Barber AR, Kentish SE, Gras SL (2010). Recent advances refining galactooligosaccharide production from lactose. Food Chem;121:307–18.

Guerrero C, Vera C, Illanes A, (2013). Optimisation of synthesis of oligosaccharides derived from lactulose (fructosyl-galacto-oligosaccharides) with β -galactosidases of different origin. Food Chemistry, 138: 2225-2232.

Günther Koraimann and , Maria Wagner A. (2014). Social behavior and decision making in bacterial conjugation. Frontier in cellular and infection microbiology, 4: 54, 1-7.

Husain Q (2010). Beta galactosidases and their potential applications: a review. Crit Rev Biotechnol;30:41–62.

Khalil, K. M. A., (2011).  Bacterial Total DNA Isolation by Alkaline Method Using Kit, Egyptian Patent Office, Academy of Scientific Research & Technology, Patent No: 25295.

Khedr MA, Emad A, Ewais, Khalil KMA (2017). Overproduction of thermophilic α-amylase productivity and Amy E gene sequence of novel Egyptian strain Bacillus licheniformisMK9 and two induced mutants. Current Science International, 6: 364-376.

Khedr MA, Desouky SE, Badr UM, Elboudy SS, Khlil KM (2013). Overproduction of β-galactosidase enzyme from Escherichia coli through genetic improvement. Journal of Applied Sciences Research, 9(8): 4809-4822.

Liu H, Bouillaut L, Sonenshein AL, Melville SB (2013). Use of a mariner-based transposon mutagenesis system to isolate Clostridium perfringens mutants deficient in gliding motility. J Bacteriol 195: 629–36.

Maksimainen M, Nina H, Johanna MK (2011). Crystal structures of Trichodermareesei β-galactosidase reveal conformational changes in the active site. Journal of Structural Biology, 174: 156-163.

Norman A, Hansen LH, Sørensen SJ (2009). Conjugative plasmids: ves sels of the communal gene pool. Philos. Trans. R. Soc. Lond. B Biol. Sci. 364, 2275–2289.

O’Connell Motherway M, O’Driscoll J, Fitzgerald G.F, Van Sinderen D (2009). Overcoming the restriction barrier to plasmid transformation and targeted mutagenesis in Bifidobacteriumbreve UCC2003.MicrobBiotechnol 2(3): 321–32.

Park AR, Oh DK (2010). Effects of galactose and glucose on the hydrolysis reaction of a thermostable beta-galactosidase from Caldicellulosiruptorsaccharolyticus. Appl Microbiol. Biotechnol; 85:1427–35.

Princely S,  Saleem N, John JK,  Dhanaraju MD  (2013). Biochemical characterization, partial purification, and production of an intracellular beta-galactosidase from Streptococcus thermophilus grown in whey. European Journal of Experimental Biology, 3: 242-251.

Ruiz L, orena; O'Connell Motherway, Mary; Lanigan, Noreen; van V, Sinderen, Douwe (2013). Transposon mutagenesis in bifidobacteriumbreve: construction and characterization of a Tn5 transposon mutant library for bifidobacteriumbreve UCC2003.PLoSONE 8(5): e64699. http://dx.doi.org/10.1371/journal.pone.0064699

Sambrook J, Fritsch EF, Maniatis T (1989). Molecular cloning. a laboratory manual. New York: Cold spring harbor laboratory press, pp 545.

Shukla, T.P., (1975). β-galactosidase technology: a solution to the lactose problem. CRC. Crit. Rev. Food Technol., 5: 325.

Volkin DB, Klibanov AM (1989). Minimizing protein inactivation. In: Creighton TE, editor. Protein function: a practical approach. Oxford: Oxford University Press, p: 1-24.

 


 

Related Articles


Original Research Articles
Emadeldin Hassan E. Konozy
Isolation, Purification and Characterization of a Thermostable β-Galactosidase isoform from Erythrina indica Seeds
Glo. Adv. Res. J. Microbiol June 2016 Vol: 5(6): - [Abstract] [Full Text - PDF] (963 KB)
Azza A El-Masry
Potential Therapeutic Effect of Curcuma longa on Streptozotocin Induced Diabetic rats
Glo. Adv. Res. J. Microbiol May 2012 Vol: 1(4): - [Abstract] [Full Text - PDF] (155 KB)
Fernando Mejia Sanchez, Marisol Rodríguez Albarrán, J. Amado López Arriaga and Julieta Castillo Cadena
Heterogeneity of GST enzymatic activity before and after treatment in patients with breast cancer: pilot study
Glo. Adv. Res. J. Microbiol July 2017 Vol: 6(7): - [Abstract] [Full Text - PDF] (311 KB)
Review
Helmy T. El Zanfaly
Biotechnology Contributions in Sustainable Environmental Development
Glo. Adv. Res. J. Microbiol January 2019 Vol: 8(1): - [Abstract] [Full Text - PDF] (189 KB)
Original Research Articles
Saffora Riaz, Farkhanda Manzoor, Nasir Mahmood, Ali Hassan, Hafsa Memona
Serum immunoglobulins (IgA, IgM, IgE) and acetylcholinesterase level determination in agricultural spray workers exposed to organophosphates pesticides in district Gujranwala
Glo. Adv. Res. J. Microbiol November 2017 Vol: 6(11): - [Abstract] [Full Text - PDF] (192 KB)
Ahmed Y Nassar, Ahmed M Ali, Mona A El-Baz, Heba M Saad Eldien, Yousif S Mohamed, Fathy F Abdel Latif, Abdel Haleem M Hussein and Muammar Y Nassar
Copper (I)-Nicotinate Complex Promoted the Synthesis of Aflatoxin M1 and Q1 More Efficiently than Butylated hydroxytoluene in Afaltoxicosed Rats
Glo. Adv. Res. J. Microbiol October 2014 Vol: 3(10): - [Abstract] [Full Text - PDF] (399 KB)

Current Issue

Viewing Options

View Full Article - PDF
Download Full Article - PDF

Search for Articles

Hind A. A. Al-Zahrani on Google Scholar
Hind A. A. Al-Zahrani on Pubmed

Statistics

Viewed 723
Printed 130
Downloaded 452
Powered By iPortal Works