Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
May 2018 Vol. 7(3): pp. 057-063
Copyright © 2018 Global Advanced Research Journals

Full Length Research Paper

Genetic mutagenesis through Transposable element 5 (Tn5) to improve beta-D-galactosidase productivity from different bacterial strains

Hind A. A. Al-Zahrani

Biology Depart., Faculty of Sciences, University of Jeddah, Jeddah, Saudi 

Email: nognagy@yahoo.com

Accepted 13 May, 2018

Abstract

β-galactosidaseIs an enzyme that catalyzes the hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides, Suicide plasmids considered a good genetic tool for DNA mutagenesis in bacterial origin specially gram negative ones, in this study a group of suicide plasmids carrying transposal element genes Tn5 and Tn7 were used for Beta-D-galactosidase productivity improvement in Escherichia coli strains through mutagenesis stimulation in genomic DNA of recipient cells for plasmid, these plasmids transferred through transconjugation mechanism as both strains related at species level. The results showed that beta-galactosidase productivity was improved in the trans-conjugated isolates which analyzed at DNA level to detect inserted plasmid through beta-galactosidase coding gene carried on it.   

Keywords:  β-galactosidase, enzyme, catalyzes, hydrolysis, non-reducing β-D-galactose.

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