Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
May 2018 Vol. 7(3): pp. 057-063
Copyright © 2018 Global Advanced Research Journals
Full Length Research Paper
Genetic mutagenesis through Transposable element 5 (Tn5) to improve beta-D-galactosidase productivity from different bacterial strains
Hind A. A. Al-Zahrani
Biology Depart., Faculty of Sciences, University of Jeddah, Jeddah, Saudi
Accepted 13 May, 2018
β-galactosidaseIs an enzyme that catalyzes the hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides, Suicide plasmids considered a good genetic tool for DNA mutagenesis in bacterial origin specially gram negative ones, in this study a group of suicide plasmids carrying transposal element genes Tn5 and Tn7 were used for Beta-D-galactosidase productivity improvement in Escherichia coli strains through mutagenesis stimulation in genomic DNA of recipient cells for plasmid, these plasmids transferred through transconjugation mechanism as both strains related at species level. The results showed that beta-galactosidase productivity was improved in the trans-conjugated isolates which analyzed at DNA level to detect inserted plasmid through beta-galactosidase coding gene carried on it.
Keywords: β-galactosidase, enzyme, catalyzes, hydrolysis, non-reducing β-D-galactose.
Bertani G (1951). Studies on lysogenesis. I. The mode of phage liberation by lysogenic Esherichia coli . J. Bacteriol., 62: 293-300.
Carla Oliveira, Pedro MR, Guimarães MR, Lucília D (2011). Recombinant microbial systems for improved β-galactosidase production and biotechnological applications. Biotechnology Advances 29: 600–609.
Gaur, R., H. Pant H, R. Jain R, and S. K. Khare SK, (2006). Galacto-oligosaccharide synthesis by immobilized Aspergillusoryzae β-galactosidase. Food Chemistry, 97(3): 426-430.
Gosling A, Stevens GW, Barber AR, Kentish SE, Gras SL (2010). Recent advances refining galactooligosaccharide production from lactose. Food Chem;121:307–18.
Guerrero C, Vera C, Illanes A, (2013). Optimisation of synthesis of oligosaccharides derived from lactulose (fructosyl-galacto-oligosaccharides) with β -galactosidases of different origin. Food Chemistry, 138: 2225-2232.
Günther Koraimann and , Maria Wagner A. (2014). Social behavior and decision making in bacterial conjugation. Frontier in cellular and infection microbiology, 4: 54, 1-7.
Husain Q (2010). Beta galactosidases and their potential applications: a review. Crit Rev Biotechnol;30:41–62.
Khalil, K. M. A., (2011). Bacterial Total DNA Isolation by Alkaline Method Using Kit, Egyptian Patent Office, Academy of Scientific Research & Technology, Patent No: 25295.
Khedr MA, Emad A, Ewais, Khalil KMA (2017). Overproduction of thermophilic α-amylase productivity and Amy E gene sequence of novel Egyptian strain Bacillus licheniformisMK9 and two induced mutants. Current Science International, 6: 364-376.
Khedr MA, Desouky SE, Badr UM, Elboudy SS, Khlil KM (2013). Overproduction of β-galactosidase enzyme from Escherichia coli through genetic improvement. Journal of Applied Sciences Research, 9(8): 4809-4822.
Liu H, Bouillaut L, Sonenshein AL, Melville SB (2013). Use of a mariner-based transposon mutagenesis system to isolate Clostridium perfringens mutants deficient in gliding motility. J Bacteriol 195: 629–36.
Maksimainen M, Nina H, Johanna MK (2011). Crystal structures of Trichodermareesei β-galactosidase reveal conformational changes in the active site. Journal of Structural Biology, 174: 156-163.
Norman A, Hansen LH, Sørensen SJ (2009). Conjugative plasmids: ves sels of the communal gene pool. Philos. Trans. R. Soc. Lond. B Biol. Sci. 364, 2275–2289.
O’Connell Motherway M, O’Driscoll J, Fitzgerald G.F, Van Sinderen D (2009). Overcoming the restriction barrier to plasmid transformation and targeted mutagenesis in Bifidobacteriumbreve UCC2003.MicrobBiotechnol 2(3): 321–32.
Park AR, Oh DK (2010). Effects of galactose and glucose on the hydrolysis reaction of a thermostable beta-galactosidase from Caldicellulosiruptorsaccharolyticus. Appl Microbiol. Biotechnol; 85:1427–35.
Princely S, Saleem N, John JK, Dhanaraju MD (2013). Biochemical characterization, partial purification, and production of an intracellular beta-galactosidase from Streptococcus thermophilus grown in whey. European Journal of Experimental Biology, 3: 242-251.
Ruiz L, orena; O'Connell Motherway, Mary; Lanigan, Noreen; van V, Sinderen, Douwe (2013). Transposon mutagenesis in bifidobacteriumbreve: construction and characterization of a Tn5 transposon mutant library for bifidobacteriumbreve UCC2003.PLoSONE 8(5): e64699. http://dx.doi.org/10.1371/journal.pone.0064699
Sambrook J, Fritsch EF, Maniatis T (1989). Molecular cloning. a laboratory manual. New York: Cold spring harbor laboratory press, pp 545.
Shukla, T.P., (1975). β-galactosidase technology: a solution to the lactose problem. CRC. Crit. Rev. Food Technol., 5: 325.
Volkin DB, Klibanov AM (1989). Minimizing protein inactivation. In: Creighton TE, editor. Protein function: a practical approach. Oxford: Oxford University Press, p: 1-24.