Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116

June 2014 Vol. 3(5), pp 083-088

Copyright © 2014 Global Advanced Research Journals

 

Full Length Research Paper

Fecal carriage of extended-spectrum β-lactamase-producing Enterobactearicae: a comparative study between hospitalized and non-hospitalized patients.

Tarek A.A. Moussa1 Rasha H. ElSherif2, Mohamed E.A. Dawoud1, Reham A. Dwedar3 and Nahla T. Muhammedy1

 

1Botany Department Science Faculty Cairo University

2Clinical Pathology Department, Faculty of Medicine, Cairo University

3Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Corresponding Author’s Email: Rasha.elsherif@kasralainy.edu.eg;

Tel: 02 0100077557; Tel Fax: 0225080099.

 

Accepted 18 June, 2014

 

Abstract

 

Recently the world has seen a surge in extended-spectrum β-lactamase (ESBL)-producing bacteria. However, data on the dissemination of ESBL-producing Enterobacteriaceae in the hospital and in non-hospitalized personal from systematically enrolled study subjects in Egypt remains few.  Aim of study: To determine the prevalence, phenotypic resistance patterns and genetic characteristics of ESBL-producing Entearobacteracicea in fecal carriage in both hospital and in non-hospitalized personal. Material and methods: A total of 160 fecal samples from 60 non hospitalized coming from out patients clinics and100 hospitalized patients .The were screened for the presence of ESBL producing Enterobactearicae. A multiplex PCR assay was used to identify blaCTX-M blaSHV, blaOXA, blaTEM genes. Results: Out of 60 clinical isolates from non-hospitalized patients group (1) it was found that the 41 Escherichia coliisolates produced the different ESBLs types as follow:(58%) produced TEM gene, (95%) produced CTX-M, (54%) produced TEM combined with CTX-M. Out of 100 clinical isolates from hospitalized patients group (II) it was founded that the (50) Escherichia coliisolates produced the different ESBLs types as follow: (78%) produced TEM gene, (96%) produced CTX-M and (74%) produced TEM combined with CTX-M. Conclusion: The most affected age was 15-30 years; this may be due to the panting of this age group on fast food, which may be the reason in the transmission and spread of infection by these microbes. blaCTX-M was the most prevalent ESBL gene followed by blaTEM and finally, blaSHV, while the blaOXA gene was not detected in any of the isolated bacteria.

Keywords: Hospitalized, non hospitalized, ESBL ,fecal.

 


 

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