Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
June 2016 Vol. 5(5): pp. 050-056
Copyright © 2016 Global Advanced Research Journals

Full Length Research Paper

Evaluation of the different diffusion capacity from a contaminated zone to adjacent areas, of two epidemic strains of K. pneumoniae OXA48–carbepenemase, using detergents or disinfectants, applied with used or unused microfiber-cloth.

Rafael Herruzo ¹, Maria Jose Vizcaino ², Irene Herruzo ³, Edward Mora ⁴

¹: MD and PhD, Catedrático de Medicina Preventiva y Salud Publica, Facultad de Medicina, Universidad Autónoma de Madrid.

²: PhD, Profesor Contratado Doctor de Microbiologia, Facultad de Medicina, Universidad Autónoma de Madrid.

³: PhD, Profesor Adjunto Universidad Francisco de Vitoria, Madrid.

⁴: PhD, Medico Adjunto Servicio de Medicina Preventiva, Hospital Universitario La Paz. Madrid.

Email: rafael.herruzo@uam.es

Accepted 27 June, 2016

Abstract

Introduction: Some disinfectants as detergent solutions, used in rooms with patients in isolation precautions, can seed to the patiens` environment multi-resistant bacteria better than chlorinate products. This fact can be evaluated through new methods of surfaces disinfection.  Methods: 1) “Immediate effect”: Four disinfectants were compared using a glass germ-carrier and Klebsiella pneumoniae with carbepenemase OXA-48 (2 strains: ST 11 and ST405). Disinfectants were applied with microfiber cloths (unused or re-used 20-30 times). Log₁₀ reductions were calculated for colony forming units (CFU) obtained after 15 min of disinfectant application. 2) Also was assessed whether these microfiber cloths (unused or reused 20-30 times), could “transfer microorganisms” to adjacent areas. Results: Sodium hypochlorite, chlorine dioxide or the mixture alcohol + quaternary ammonium compounds (QUACs) produced complete destruction of all used microorganisms. Moreover diffusion of microorganisms to around area was none o very little. Nevertheless, the diluted quaternary ammonium permitted K pneumoniae-OXA48 diffusion to adjacent areas, through the re-used microfiber cloth. With unused microfiber, the results improve, but did not eliminate this diffusion to around area. Conclusion:  Two types of tests should be performed before advising surface disinfectant of hospital rooms of patients on contact precautions: 1) direct effect and 2) evaluation of the possibility of transfer of microorganisms by the used or unused microfiber. In our case, chlorine dioxide, hypochlorite or alcohol + QUACs must be preferred to QUACs, in rooms´ disinfection of patients with K. pneumoniae OXA48.

Keywords: Evaluation, surface-disinfection, microfiber, K. pneumoniae-OXA48 carbepenemase.