Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
June 2018 Vol. 7(4): pp. 064-072
Copyright © 2018 Global Advanced Research Journals


Full Length Research Paper


Isolation of Aerobic Bacteria from Wounds of Diabetic Patients Undergoing Hyperbaric Oxygen Therapy in Khartoum State

1*Ahmed Ibrahim Hashim, 1Amal Mubarak Hassan Mubarak, 1Nosiba Ahmed Hassan Hamed, and 2*Ahmed Bakheet Abd Alla


1*Department of Microbiology- College of Medical Laboratory Science, Sudan University of Science and Technology

2Department of Parasitology and Medical Entomology -College of Medical Laboratory Science, Sudan University of Science and Technology



Accepted 07 June, 2018



Background: Diabetic wound infection is one of the complications of diabetes mellitus, hyperbaric Oxygen Therapy (HBOT) can be used as an adjacent treatment for diabetic wound infection, it involves inhalation of 100% oxygen under a pressure greater than 1 atmospheric absolute. This was a longitudinal case study, in which the wound specimens were collected from AL-Dirby Centre and further processing was done at the Microbiology Laboratory, Sudan University of Science and Technology (SUST), between January and July 2016. Objective: The aim of this study was to evaluate the effect of hyperbaric oxygen therapy (HBOT) on aerobic bacteria isolated from the wounds of diabetic patients attending Al-Dirby Center. Methodology: A total of 22 diabetic patients were involved in this study, their ages between 40 and 98 years. The majority of the patients were males 17 (77.3 %) and 5 (22.7%) were females, Insulin dependent diabetic patients were 15 (68.2%) and insulin independent diabetic patients were 7 (31.8%). A Questionnaire was used to collect data from the patients. The data was analyzed by using SPSS version (11.5). The results showed (78.1%) Gram-negative rods and (21.9%) Gram- positive cocci. The most frequent isolated bacteria were Proteus mirabilis 8(25%) followed by Pseudomonas aeruginosa 6(18.7%), Staphylococcus aureus and Escherichia coli 5 (15%), Proteus vulgaris 4(12.5%), and (Sterptococcus pyogenes, Serratia marcescens, Viridans streptococci, and Klebsiella pneumoniae) 1 (3.1%). Gram negative rods were more sensitive to Imipenem while Gram positive cocci were more sensitive to Gentamicin. Complete wound healing and bacterial eradication was observed in 17(77.3%) of the diabetic patients while incomplete wound healing and bacterial persistence was observed in only 5(22.7%). Conclusion: This study showed that HBOT could be a useful tool in woundstherapy.

Keywords:  Diabetic wound, hyperbaric Oxygen Therapy, AL-Dirby, Proteus mirabilis.





Abbass EI (2009). Frequency and Sensitivity Pattern of Aerobic Bacteria isolated from Wounds of Diabetic Patients. M.Sc thesis. Sudan University of Science and Technology.

Abdel Wahab WF, Bakhiet MA, Mahadi SI, Mahmoud SM, Widataal AH, Ahmed ME (2013). Diabetic Foot Infections with Pseudomonas: Jabir Abueliz Diabetic Center Khartoum Experience. Clinical Research on Foot & Ankle. 83(S3):1-3.

Ahmed EO (2003). Bacteriology of Diabetic Foot Infections in Sudan. Clinical MD thesis. University of Khartoum.

Akhi MT, Ghotaslou R, Asgharzadeh M, Varshochi M, Pirzadeh T, Memar MY (2015). Bacterial etiology and antibiotic susceptibility pattern of diabetic foot infections in Tabriz, Iran. GMS Hygieneand Infection Control, 10:1-6.

Ali AAS (2005). Isolation and identification of bacteria associated with diabetic foot infections. M.Sc thesis. University of Khartoum.

Al-Saimary IA (2010). Bacterial wound infections in diabetic patients and their therapeutic implications, Medical Practice and Review. 1 (2): 12-15.

Barata P, Cervaens M, Resende R, Camacho O, Marques F (2011). Hyperbaric oxygen effects on sports injuries. Therapeutic Advances in Musculoskeletal Disease 3 (2): 111-121.

Benjamin LA (2010). Hyperbaric Oxygen Therapy for Diabetic Foot Wound. Diabatic Care, 33(5):1143-1145.20-

Bhutani S, Vishwanath G (2012). Hyperbaric oxygen and wound healing. Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India, 45(2), 316.

Camporesi EM (2014).Side effects of hyperbaric oxygen therapy. Journal of the Undersea and Hyperbaric Medical Society, 41 (3):253-257.

Davies S, Tovey M (2014). Investigation of wound, tissue, and genital samples. Medical Microbiology, 366(11), 1028-1037.

DiNubile MJ, Lipsky BA (2004). Complicated infections of skin and skin structures: when the infection is more than skin deep. Journal of Antimicrobial Chemotherapy, 53(suppl_2), ii37-ii50.

Doctor A (1992). Hyperbaric oxygen therapy in diabetic foot. Journal of Postgraduate Medicine, 38(3),112-114.

Drake DR, Brogden KA, Dawson DV, Wertz PW (2008). Thematic review series: skin lipids. Antimicrobial lipids at the skin surface. Journal of lipid research, 49(1), 4-11.

Dryden MS (2010). Complicated skin and soft tissue infection. Journal of Antimicrobial chemotherapy, 65(suppl_3), iii35-iii44.

Ekmektzoglou KA, Zografos GC (2006). A concomitant review of the effects of diabetes mellitus and hypothyroidism in wound healing. World Journal of Gastroenterology: WJG, 12(17), 2721.

El-Tahawy AT (2000). Bacteriology of diabetic foot Infections. Saudi Medical Journal, 21 (4):344-347.

Ford M (2010). Medical Microbiology, 1st edition, Oxford University Press, New York, p 143.

Goering RV, Dockrell HM, Zuckerman M, Roitt IM, Chiodini PL (2013). Mims' Medical Microbiology, fifth edition, Elsevier saunders, China, p335.

Greenwood D, Slack R, Peutherer J, Barer M (2007). Medical Microbiology, seventeenth edition, Churchill livingstoneelsevier, China, p 662.

Huang ET, Mansouri J, Murad MH, Joseph WS, Strauss MB, Tettelbach W, Worth ER (2015). A clinical practice guideline for the use of hyperbaric oxygen therapy in the treatment of diabetic foot ulcers. Undersea Hyperb Med, 42(3),205-247.

Koreck A, Pivarcsi A, Dobozy A, Kemeny L (2003). The role of innate immunity in the pathogenesis of acne. Dermatology, 206(2), 96-105.

Levinson, W. (2008). Review of Medical Microbiology and Immunology,

tenth edition, Mc-Graw- Hill Companies, Sanfrancisco, p 66.

Mahgoub EM, Omer MEA (2015). Aerobic bacteria isolated from diabetic septic wounds. American Journal of Research Communication. 3 (10): 91-99.

Mandell GL, Bennett JE, Dolin R (2010). Mandell, Douglass and Bennett's Principles and Practice of Infectious Diseases, Seventh Edition, Mandell, Douglas, and Bennetts Principles and Practice of Infectious Diseases, Churchill Livingstone, Elsevier, Philadelphia, USA, pp 278-279

Mohanasoundaram KM (2012). The Microbiological Profile of Diabetic Foot Infections. Journal of Clinical and Diagnostic Research. 6 (3): 409-411.

Mordi RM, Momoh MI (2009). Incidence of Proteus species in wound infections and their sensitivity pattern in the University of Benin Teaching Hospital. African Journal of Biotechnology.8(5):725-730.

Ong M (2008). Hyperbaric oxygen therapy in the management of diabetic lower limb wounds. Singapore medical journal, 49(2),105.

Perim MC, Borges JC, Celeste SRC, Orsolin EF, Mendes RR, Mendes GO (2015). Aerobic bacterial profile and antibiotic resistance in patients with diabetic foot infections. Revistada Sociedade de MedicinaTropica, 48 (5):546-554.

Rao C, Xiao L, Liu H, Li S, Lu J, Li J, Gu S (2016). Effects of topical oxygen therapy on ischemic wound healing. The Journal of Physical Therapy Science, 28 (1):118-123.

Rhody C (2000). Bacterial infections of the skin. Primary Care: Clinics in Office Practice, 27(2),459-473.

Sugandhi P, Prasanth DA (2007). Bacteriological Profile of Diabetic Foot Infections. International Journal of Innovative Research in Science, Engineering and Technology. 3 (7): 14688-14692.

Tille PM (2014). Diagnostic Microbiology, thirty edition, chapter 76, Elsevie, China, p961,

Tognetti L, Martinelli C, Berti S, Hercogova J, Lotti T, Leoncini F, Moretti S (2012). Bacterial skin and soft tissue infections: review of the epidemiology, microbiology, aetiopathogenesis and treatment. Journal of the European Academy of Dermatology and Venereology, 26(8),931-941.

Turhan V, Mutluoglu M, Acar A, Hatipo─člu M, Önem Y, Uzun G (2013). Increasing incidence of Gram-negative organisms in bacterial agents isolated from diabetic foot ulcers. The Journal of infection in Developing Countries. 7 (10):707-712.

Venus M, Waterman J, McNab I (2010). Basic physiology of the skin.

Surgery-Oxford International Edition, 28(10), 469-472.

Yutsis PI (2003). Oxygen to the Rescue: Oxygen Therapies, and How They Help Overcome Disease, Promote Repair, and Improve  Overall Function. Basic Health Publications,Inc..



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