Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
June 2018 Vol. 7(4): pp. 073-083
Copyright © 2018 Global Advanced Research Journals


Full Length Research Paper


Solid state fermentation using agro industrial residues for alpha amylase production by an actinomycete isolate from red sea soil

Hind A. A. Al-Zahrani


Biology Department, Faculty of Sciences, University of Jeddah, Jeddah, Saudi Arabia



Accepted 13 May, 2018



During the screening process to obtain the most potent α-amylase actinomycete producer from soil in red sea region, Streptomyces sp.HA2 was isolated and identified as working strain in solid state fermentation using agro industrial residues. Investigation of four agricultural residues such as banana peel, sugarcane bagasse, wheat bran and rice bran to select the best substrate for production of α-amylase. Selection of banana peel as substrate improved the activity. Various process parameters were optimized for maximum amylase production resulted in incubation period of (96 h), pH (8.5), inoculum size (8%), and moisture content (40%). Also, production of 1500 u/gds was achieved by supplementing the media with 1% starch and 1% peptone on 10g banana peel. The partially purified enzyme by ammonium sulphate was found stable at temperature up to 50°C for 120 minute. By using these optimized cultural conditions, this α-amylase may be utilized in wide spread applications like detergent, leather, and agriculture, scarification, pharmaceutical industry as well as molecular biology techniques..   

Keywords:  alpha amylase , Solid state fermentation, Streptomyces sp,  16S rDNA, agro industrial residues.





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Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
June 2018 Vol. 7(4): pp. 064-072
Copyright © 2018 Global Advanced Research Journals


Full Length Research Paper


Isolation of Aerobic Bacteria from Wounds of Diabetic Patients Undergoing Hyperbaric Oxygen Therapy in Khartoum State

1*Ahmed Ibrahim Hashim, 1Amal Mubarak Hassan Mubarak, 1Nosiba Ahmed Hassan Hamed, and 2*Ahmed Bakheet Abd Alla


1*Department of Microbiology- College of Medical Laboratory Science, Sudan University of Science and Technology

2Department of Parasitology and Medical Entomology -College of Medical Laboratory Science, Sudan University of Science and Technology



Accepted 07 June, 2018



Background: Diabetic wound infection is one of the complications of diabetes mellitus, hyperbaric Oxygen Therapy (HBOT) can be used as an adjacent treatment for diabetic wound infection, it involves inhalation of 100% oxygen under a pressure greater than 1 atmospheric absolute. This was a longitudinal case study, in which the wound specimens were collected from AL-Dirby Centre and further processing was done at the Microbiology Laboratory, Sudan University of Science and Technology (SUST), between January and July 2016. Objective: The aim of this study was to evaluate the effect of hyperbaric oxygen therapy (HBOT) on aerobic bacteria isolated from the wounds of diabetic patients attending Al-Dirby Center. Methodology: A total of 22 diabetic patients were involved in this study, their ages between 40 and 98 years. The majority of the patients were males 17 (77.3 %) and 5 (22.7%) were females, Insulin dependent diabetic patients were 15 (68.2%) and insulin independent diabetic patients were 7 (31.8%). A Questionnaire was used to collect data from the patients. The data was analyzed by using SPSS version (11.5). The results showed (78.1%) Gram-negative rods and (21.9%) Gram- positive cocci. The most frequent isolated bacteria were Proteus mirabilis 8(25%) followed by Pseudomonas aeruginosa 6(18.7%), Staphylococcus aureus and Escherichia coli 5 (15%), Proteus vulgaris 4(12.5%), and (Sterptococcus pyogenes, Serratia marcescens, Viridans streptococci, and Klebsiella pneumoniae) 1 (3.1%). Gram negative rods were more sensitive to Imipenem while Gram positive cocci were more sensitive to Gentamicin. Complete wound healing and bacterial eradication was observed in 17(77.3%) of the diabetic patients while incomplete wound healing and bacterial persistence was observed in only 5(22.7%). Conclusion: This study showed that HBOT could be a useful tool in woundstherapy.

Keywords:  Diabetic wound, hyperbaric Oxygen Therapy, AL-Dirby, Proteus mirabilis.





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