Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
June 2019 Vol. 8(4): pp. 063-071
Copyright © 2019 Global Advanced Research Journals

 

Full Length Research Paper

 

Genetic Characterization of Campylobacter jejuni Isolated from Boiler Flocks

H. Abo Al-Yazeed1, Amani M. Marwan1, Attia, A. M.2, A. Samir1, Ammar, A. M3

1Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.

2 Department of Bacteriology, Animal Health Research Institute, Dokki, Giza.

3 Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt.

Email: amanymarwan110@mail.com

Accepted 05 June, 2019

 

Abstract

Campylobacter  is a worldwide infection which has been estimated it as the most significant economic burden by EFSA and ECDC in 2016. It caused mainly by either Campylobacter  jejuni or Campylobacter coli. Poultry are a natural reservoir for Campylobacter species, although it is insignificant for poultry health and is a leading cause of gastroenteritis for humans in developed and developing countries. The disease is endemic in Egypt and is a major cause for diarrhea in children. Good understanding of epidemiology and surveillance of Campylobacter will help in elimination and prevention of it among animals and humans. Many molecular typing techniques used to track the source of infection and reduce Campylobacter infection rate. To achieve that, 290 samples were collected from broiler flocks and slaughter market from Cairo governorate, Egypt. Bacteriological and molecular identification have been implemented based on genus and species level for C. jejuni then phylogenetic tree analysis of flaA gene to correlate the genetic relatedness and emphasize that chicken is the major source of infection.

Keywords:  Campylobacter jejuni, genotyping, flaA gene, short variable region, chicken, Human.

 

 

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