Global Advanced Research Journal of Microbiology (GARJM) ISSN: 2315-5116
May 2016 Vol. 5(6): pp. 057-067
Copyright © 2016 Global Advanced Research Journals
Full Length Research Paper
Distribution of free-living amoebae in springs in Morelos, Mexico.
Elizabeth Ramirez1, Esperanza Robles1, Maria E. Martinez1, Maria G. Sainz1, Blanca Martinez1, Blanca I. Rivas1 and Arturo Rocha2
1Proyecto de Conservación y Mejoramiento del Ambiente. Facultad de Estudios Superiores Iztacala. Universidad Nacional Autónoma de México. Avenida de los Barrios 1, Los Reyes Iztacala. Tlalnepantla 54090 Estado de México. México.
2Laboratorio de Ecología, Facultad de Estudios Superiores Iztacala. Universidad Nacional Autónoma de México. Avenida de los Barrios 1, Los Reyes Iztacala. Tlalnepantla 54090 Estado de México. México.
Accepted 16 August, 2016
Free-living amoebae (FLA) can accomplish their life cycle as free-living organisms; therefore it is important to know more about the distribution of FLA in the environment. The aim of the study was to determine the presence and distribution of free-living amoebae in recreational and drinking water springs. Eight springs were selected and the samples were taken on a bimonthly basis for one year. Filtration of water samples was performed and culture was done on non-nutrient agar medium with Enterobacter aerogenes bacteria. Identification of FLA was carried out taking in account the morphological characteristics of the trophozoite and cyst. Forty one of 48 (85.4%) samples tested positive for the presence of free-living amoebae and isolated amoebae belonging to 15 genera. Genera were grouping into 4 groups in according to spatial-seasonal distribution, having Naegleria and Vermamoeba the highest frequency and distribution. The study contributed to knowledge of the diversity of free-living amoebae in springs and provided interesting information about the spatial and seasonal distribution FLA in these water bodies.
Keywords: free-living amoebae, Naegleria, Vermamoeba, springs, recreational water.
- Elizabeth Ramirez on Google Scholar
- Elizabeth Ramirez on Pubmed
- Esperanza Robles on Google Scholar
- Esperanza Robles on Pubmed
- Maria E. Martinez on Google Scholar
- Maria E. Martinez on Pubmed
- Maria G. Sainz on Google Scholar
- Maria G. Sainz on Pubmed
- Blanca Martinez on Google Scholar
- Blanca Martinez on Pubmed
- Blanca I. Rivas on Google Scholar
- Blanca I. Rivas on Pubmed
- Arturo Rocha on Google Scholar
- Arturo Rocha on Pubmed